Fibronectin Antibody (CL3730) - BSA Free

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line U-87 MG

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human pancreas shows absence of immunoreactivity in glandular cells (negative control).

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human placenta shows strong immunoreactivity in chorionic villi.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human testis shows immunoreactivity in the connective tissue surrounding seminiferous tubules.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human prostate shows moderate positivity in smooth muscle.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human small intestine shows strong immunoreactivity in the connective tissue.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human breast cancer shows strong immunoreactivity in tumor stroma.

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human liver cancer shows strong positivity in tumor.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL3730
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Fibronectin Antibody (CL3730) - BSA Free Summary

• Description: Novus Biologicals Mouse Fibronectin Antibody (CL3730) - BSA Free (NBP2-61633) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P02751, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: IHC-Paraffin HIER pH6 retrieval is recommended.
• Control Peptide:

  Fibronectin Recombinant Protein Antigen (NBP2-61633PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Fibronectin Antibody (CL3730) - BSA Free

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FN1