Human Fibrillin 1 ELISA Kit (Colorimetric)

ELISA: Human Fibrillin 1 ELISA Kit (Colorimetric) [NBP3-25945] - Standard Curve Reference

ELISA: Human Fibrillin 1 ELISA Kit (Colorimetric) [NBP3-25945] - Samples were spiked with high concentrations of Human Fibrillin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL

Human Fibrillin 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Fibrillin 1 in samples. No significant cross-reactivity or interference between Human Fibrillin 1 and analogues was observed
• Standard Curve Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 6.90
• Intra-Assay: %CV < 6.67
• Spike Recovery: 93-96%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FBN1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Fibrillin 1 ELISA Kit (Colorimetric)

• ACMICD
• Asprosin
• FBN
• FBN1
• fibrillin 1 (Marfan syndrome)
• Fibrillin 1
• fibrillin 15
• fibrillin-1
• GPHYSD2
• MASS
• MFS1
• OCTD
• SGS
• SSKS
• WMS
• WMS2

Background

Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Fibrillin 1 ELISA Kit (NBP3-25945). (Showing 1 - 2 of 2 FAQ).

1. Hi - I have been looking at the fibrillin1 antibodies on your website. You indicate that some may be suitable for canine tissue on your website. could you let me know which of the 10 antibodies is the most suitable for canine.
   • We have unfortuantely not tested any of our products in canine samples. If you would like to try one of our product with canine samples, you would qualify for our Innovator's Reward Program. In exchange for a review of our product in an untested application or species, we would issue you a credit voucher for the purchase price. If you would like us to check the homology of a certain product against the canine sequence, just contact our technical support department at technical@novusbio.com.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FBN1