Fibrillin 1 Antibody (11C1.3)

Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Frozen: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Adult eye (blue) and muscle (red) tissue. An image was captured using epifluorescent microscope detecting signals from Alexa fluor 568 conjugated secondary antibody. Used at 1:100 dilution. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).

Product Discontinued

Fibrillin 1 Antibody (11C1.3) Summary

• Immunogen: Microfibrils from zonular apparatus of bovine eye
• Epitope: Amino acids 451 - 909.
• Localization: Extracellular microfibrils.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunomicroscopy 1:10 - 1:500
  • Western Blot 1 - 2 ug/ml

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 17599682).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for Fibrillin 1 Antibody (11C1.3)

• ACMICD
• Asprosin
• FBN
• FBN1
• fibrillin 1 (Marfan syndrome)
• Fibrillin 1
• fibrillin 15
• fibrillin-1
• GPHYSD2
• MASS
• MFS1
• OCTD
• SGS
• SSKS
• WMS
• WMS2

Background

Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibrillin 1 Antibody (NB110-8146)(5)

Publications using NB110-8146

• Ramakrishnan R, Venkiteswaran K, Sreelatha H et al. Assembly of skin substitute by cross-linking natural biomaterials on synthetic biodegradable porous mat for critical-size full-thickness burn wound regeneration Biomedical Materials 2022-03-01 [PMID: 35168228]
• Shin S, Lee J, Yoon SH Et al. Anti-glycation activities of methyl gallate in vitro and in human explants Journal of cosmetic dermatology 2021-08-21 [PMID: 34418257] (IHC-Fr, Human)
• Ajit A, Ramakrishnan R, Retnabai ST et al. Generation of niche tuned antifibrotic fibroblasts and non-viral mediated endothelial commitment using adipose stem cells for dermal graft development J. Biomed. Mater. Res. Part B Appl. Biomater. 2020-04-03 [PMID: 32243682]
• Shin S, Son D, Kim M et al. Ameliorating Effect of Akebia quinata Fruit Extracts on Skin Aging Induced by Advanced Glycation End Products. Nutrients. 2015-11-17 [PMID: 26569300] (IHC-Fr, Human)
• Shin S, Lee JA, Kim M et al. Anti-Glycation Activities of Phenolic Constituents from Silybum marianum (Milk Thistle) Flower in Vitro and on Human Explants Molecules. 2015-02-24 [PMID: 25706757] (IHC-Fr)

Review for Fibrillin 1 Antibody (NB110-8146) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Feline.

reviewed by: Verified Customer IHC-Fr Feline 06/05/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult eye and muscle tissue (labeled E and M, respectively)
• Species: Feline
• Lot: TE2577931A

Comments

• Comments: Used at 1:100 dilution

FAQs for Fibrillin 1 Antibody (NB110-8146). (Showing 1 - 1 of 1 FAQs).

1. Hi - I have been looking at the fibrillin1 antibodies on your website. You indicate that some may be suitable for canine tissue on your website. could you let me know which of the 10 antibodies is the most suitable for canine.
   • We have unfortuantely not tested any of our products in canine samples. If you would like to try one of our product with canine samples, you would qualify for our Innovator's Reward Program. In exchange for a review of our product in an untested application or species, we would issue you a credit voucher for the purchase price. If you would like us to check the homology of a certain product against the canine sequence, just contact our technical support department at technical@novusbio.com.

Bioinformatics

• Gene Symbol: FBN1
• Entrez:
  • 2200(Human)
• Uniprot:
  • P35555()