Fibrillarin Antibody (28F2)

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Western Blot: Fibrillarin Antibody (28F2) [NBP1-05223] - Western Blot analysis of whole yeast protein extracts detect Fibrillarin/Nop1p. Molecular weight detected is 34.5 kDa.

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF, IP
Clone
28F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Fibrillarin Antibody (28F2) Summary

Immunogen
Yeast nuclear preparation (S. cerevisiae).
Marker
Nucleolar Marker
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
FBL
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000 (cell lysates)-1:25000 (nuclear fractions)
  • Immunocytochemistry/Immunofluorescence 1:2000-1:20000
  • Immunoprecipitation 2-4 ul
Application Notes
This Fibrillarin antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Purity
Tissue culture supernatant

Alternate Names for Fibrillarin Antibody (28F2)

  • EC 2.1.1,34-kD nucleolar scleroderma antigen
  • EC 2.1.1.-
  • EC 2.1.1.37
  • FIB
  • FIB1,34 kDa nucleolar scleroderma antigen
  • fibrillarin
  • FLRNrRNA 2'-O-methyltransferase fibrillarin
  • RNA, U3 small nucleolar interacting protein 1
  • RNU3IP1

Background

Fibrillarin (FBL; human homologue of yeast Nop1p) is the most abundant proteins in fibrillar regions of the eukaryotic cell nucleolus, the site for early phase of pre-rRNA processing, and is considered an excellent marker for nucleolus. FBL interacts with several proteins such as NOLC1, DDX5, C1QBP, PRMT5 and UTP20; and with U3, U8, U13, X and Y small nuclear RNAs also. FBL forms a component of several ribosomal/nucleolar protein complexes and of snoRNP (box C/D small nucleolar ribonucleoprotein) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. In pre-ribosomal RNA, FBL utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties, the site specificity being offered by the guide RNA that base pairs with the substrate, and the methylation occurs at a unique distance from the sequence involved in base pairing with the guide RNA. In humans, FBL also acts as a nucleolar autoantigen for non-hereditary immune disease scleroderma, a disease in which the patients have strong circulating FBL autoantibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibrillarin Antibody (NBP1-05223). (Showing 1 - 1 of 1 FAQ).

  1. What is the sodium azide percentage for this product?
    • No preservative 

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Bioinformatics

Gene Symbol FBL
Entrez