FGFR1OP Antibody (2B1)

Western Blot: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M01), clone 2B1. Lane 1: FGFR1OP transfected lysate(43.1 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of monoclonal antibody to FGFR1OP on HeLa cell. Antibody concentration 10 ug/mL.

Immunohistochemistry-Frozen: FGFR1OP Antibody (2B1) [H00011116-M01] - Rabbit frozen 4% paraformalehyde fixed brain sections. Double labeling with FGFR1OP (Basal bodies in green) and GT335 (cilia in red) antibodies of ependymal whole mount from brain of rabbit kit. IHC-Fr image submitted by a verified customer review.

ELISA: FGFR1OP Antibody (2B1) [H00011116-M01] - Detection limit for recombinant GST tagged FGFR1OP is approximately 0.03 ng/mL as a capture antibody.

• Reactivity: Hu, Mu, Rb
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr
• Clone: 2B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGFR1OP Antibody (2B1) Summary

• Immunogen: FGFR1OP (AAH11902, 1 a.a ~ 379 a.a) full length recombinant protein with GST tag.MAATAAAVVAEEDTELRDLLVQTLENSGVLNRIKAELRAAVFLALEEQEKVENKTPLVNESLRKFLNTKDGRLVASLVAEFLQFFNLDFTLAVFQPETSTLQGLEGRENLARDLGIIEAEGTVGGPLLLEVIRRCQQKEKGPTTGEGALDLSDVHSPPKSPEGKTSAQTTPSKKANDEANQSDTSVSLSEPKSKSSLHLLSHETKIGSFLSNRTLDGKDKAGLCPDEDDMEGDSFFDDPIPKPEKTYGLRNEPRK
• Specificity: FGFR1OP - FGFR1 oncogene partner
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEP43
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It is also useful for immunofluorescence. This FGFR1OP Antibody (2B1) is validated for IHC-Fr from a verified customer review.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28625565). Other species not tested. Rabbit reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FGFR1OP Antibody (2B1)

• FGFR1 oncogene partner
• FOPfibroblast growth factor receptor 1 oncogene partner

Background

This gene encodes a largely hydrophilic protein postulated to be a leucine-rich protein family member. A t(6;8)(q27;p11) chromosomal translocation, fusing this gene and the fibroblast growth factor receptor 1 (FGFR1) gene, has been found in cases of myeloproliferative disorder. The resulting chimeric protein contains the N-terminal leucine-rich region of this encoded protein fused to the catalytic domain of FGFR1. This gene is thought to play an important role in normal proliferation and differentiation of the erythroid lineage. Alternatively spliced transcript variants that encode different proteins have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1OP Antibody (H00011116-M01)(6)

Publications using H00011116-M01

• Hilgendorf KI, Johnson CT, Mezger A et al. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis Cell 2019-11-27 [PMID: 31761534]
• Ortiz-Alvarez G, Daclin M, Shihavuddin A et al. Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members. Neuron. 2019-02-26 [PMID: 30824354]
• Mahuzier A, Shihavuddin A, Fournier C et al. Ependymal cilia beating induces an actin network to protect centrioles against shear stress. Nat Commun 2018-06-11 [PMID: 29891944]
• kanie T, Abbott kL, MooneY NA et al. The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev. Cell. 2017-07-10 [PMID: 28625565] (ICC/IF, Mouse)
• Boutin C, Labedan P, Dimidschstein J et al. A dual role for planar cell polarity genes in ciliated cells. Proc Natl Acad Sci U S A. 2014-07-14 [PMID: 25024228]
• Mano Y, Takahashi K, Ishikawa N et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 2007-09-20 [PMID: 17888034]

Bioinformatics

• Gene Symbol: CEP43
• Entrez:
  • 11116(Human)
• OMIM:
  • 605392(Human)
• Uniprot:
  • AAH11902(Human)