FGFR1 Antibody (M2F12)

Western Blot: FGF R1 Antibody (M2F12) [NB120-829] - Total protein from Ntera-2 was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/ml of anti-FGFR1. FGFR1 protein was detected at approximately 150 kDa using an anti-mouse HRP secondary antibody.

Immunocytochemistry/Immunofluorescence: FGF R1 Antibody (M2F12) [NB120-829] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with FGR1 (M2F12) (NB120-829) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in HEK293 cells (2 x10^6) stained with FGF R1 antibody (NB120-829, red) at a 1:1000 dilution and detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP, CyTOF-ready
• Clone: M2F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FGFR1 Antibody (M2F12) Summary

• Immunogen: Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
• Epitope: NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
• Specificity: Reacts with alpha isoform of FRFR1. Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
• Application Notes: Western blotting: 1 ug/mL recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: Native, nuclear, recombinant, and ligand-labeled FGFr1. Customers have reported difficulties using this antibody in immunocytochemistry staining (see review). This antibody does not appear to work in IHC paraffin embedded sections. Use in Immunohistochemistry reported in scientific literature (PMID: 8168112).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FGFR1 Antibody (M2F12)

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1 Antibody (NB120-829)(3)

Publications using NB120-829

• Wang F, Kan M, McKeehan K et al. A homeo-interaction sequence in the ectodomain of the fibroblast growth factor receptor. J Biol Chem. 1997 Sep 19 [PMID: 9295338] (WB)
• Morrison RS, Yamaguchi F, Bruner JM et al. Fibroblast growth factor receptor gene expression and immunoreactivity are elevated in human glioblastoma multiforme. Cancer Res. 1994 May 15 [PMID: 8168112] (IHC, Human)
• Zhao XM, Frist WH, Yeoh TK, Miller GG. Modification of alternative messenger RNA splicing of fibroblast growth factor receptors in human cardiac allografts during rejection. J Clin Invest. 1994 Sep [PMID: 7521891] (IHC, Human)

FAQs for FGFR1 Antibody (NB120-829). (Showing 1 - 2 of 2 FAQs).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Bioinformatics

• Gene Symbol: FGFR1
• Entrez:
  • 2260(Human)
  • 14182(Mouse)
  • 79114(Rat)