FGFR1 Antibody (M17A3)

Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IHC-P, IP, CyTOF-ready
• Clone: M17A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FGFR1 Antibody (M17A3) Summary

• Immunogen: Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
• Epitope: Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.
• Localization: Cell Membrane
• Specificity: Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/ml
  • Flow Cytometry 1:500-1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Human, mouse and rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FGFR1 Antibody (M17A3)

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Background

FGF R1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGF R1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGF R1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGF R1 result in Pfeiffer syndrome associated with craniosynostosis. FGF R1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGF R1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1 Antibody (NB100-2079)(1)

Publication using NB100-2079

• Wang F, Kan M, McKeehan K et al. A homeo-interaction sequence in the ectodomain of the fibroblast growth factor receptor. J Biol Chem. 1997 Sep 19 [PMID: 9295338] (WB)

FAQs for FGFR1 Antibody (NB100-2079). (Showing 1 - 3 of 3 FAQ).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.
3. Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?
   • In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

Bioinformatics

• Gene Symbol: FGFR1
• Entrez:
  • 2260(Human)
• Uniprot:
  • P11362()