Mouse FGF basic/FGF2 ELISA Kit (Colorimetric)

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ELISA: Mouse FGF basic/FGF2 ELISA Kit (Colorimetric) [KA1777] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum
Sensitivity
8 pg/mL

Order Details

Mouse FGF basic/FGF2 ELISA Kit (Colorimetric) Summary

Description
Fgf2 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Fgf2.
Sensitivity
8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FGF2

Applications/Dilutions

Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. HRP-Streptavidin concentrates (Item G): 300x concentrated HRP-conjugatedStreptavidin.
  2. Detection Antibody bFGF (Item F): Biotinylated anti-mouse bFGF (each vial isenough to assay half microplate).
  3. bFGF Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mousebFGF.
  4. Assay Diluent B (Item E): 5x concentrated buffer.
  5. Assay Diluent A (Item D): Diluent buffer, 0.09% sodium azide as preservative.For Standard/Sample (serum/plasma) diluent.
  6. Stop Solution (Item I): 0.2 M sulfuric acid.
  7. Standard Protein (Item C): Mouse bFGF. One vial is enough to run eachstandard in duplicate.
  8. TMB One-Step Substrate Reagent (Item H): of 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

Quality control test: Standard Curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse FGF basic/FGF2 ELISA Kit (Colorimetric)

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for FGF basic/FGF2 ELISA Kit (KA1777). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2
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