Human FGF basic/FGF2 ELISA Kit (Colorimetric)

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ELISA: Human FGF basic/FGF2 ELISA Kit [KA0527] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum
Standard Curve Range
78 to 8000 pg/mL
Sensitivity
78 pg/mL

Order Details

Human FGF basic/FGF2 ELISA Kit (Colorimetric) Summary

Description
FGF2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human FGF2.
Suitable Sample and Volume: Cell Culture Supernatant, Plasma, Serum, 100 uL
Standard Curve Range
78 to 8000 pg/mL
Sensitivity
78 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 4.69%
Intra-Assay
CV% < 6.39%
Spike Recovery
Serum: 94.00%, Plasma: 102.00%, Cell Culture Supernatant: 108.00%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FGF2

Applications/Dilutions

Application Notes
FGF2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human FGF2.
Publications
Read Publications using KA0527.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. CALIBRATOR DILUENT I
  2. CALIBRATOR DILUENT II
  3. BIOTIN CONJUGATE
  4. AVIDIN CONJUGATE
  5. Antibody coated microwells
  6. STANDARD
  7. Stop Solution
  8. WASH BUFFER (20X)
  9. SUBSTRATE A
  10. SUBSTRATE B

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human FGF basic/FGF2 ELISA Kit (Colorimetric)

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for FGF basic/FGF2 ELISA Kit (KA0527)(3)

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Product General Protocols

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FAQs for FGF basic/FGF2 ELISA Kit (KA0527). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2
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