FGF basic/FGF2/bFGF Antibody [Unconjugated]

Recombinant Human FGF basic/FGF2/bFGF 146 aa (Catalog # 233-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF basic/FGF2/bFGF 146 aa (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic/FGF2/bFGF Polyclonal Antibody (Catalog # AB-233-NA). The ND50 is typically 0.5‑1.0 µg/mL.

Product Discontinued

FGF basic/FGF2/bFGF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FGF basic/FGF2/bFGF
• Specificity: Detects human FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human FGF acidic is observed. Neutralizes the biological activity of recombinant human FGF basic/FGF2/bFGF. It will also neutralize the biological effects of bovine FGF basic/FGF2/bFGF, but it has no effect on recombinant human or bovine FGF acidic.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF2
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.5-1.0 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF basic/FGF2/bFGF Antibody [Unconjugated]

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike theother members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic hasbeen isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues,but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are producedas a result of Nterminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGFto heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to thesame high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cellsof neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonicdevelopment and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normalprocesses as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally,FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF basic/FGF2/bFGF Antibody (AB-233-NA)(5)

Publications using AB-233-NA

• GY Zhang, EA Langan, NT Meier, W Funk, F Siemers, R Paus Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo PLoS ONE, 2019-03-29;14(3):e0212659. 2019-03-29 [PMID: 30925152] (Neutralization, Human)
• Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity. Nat Med, 2015-04-20;21(5):530-6. 2015-04-20 [PMID: 25894825] (Neutralization, Human)
• Boku , Shuken, Hisaoka-Nakashima , Kazue, Nakagawa , Shin, Kato , Akiko, Kajitani , Naoto, Inoue , Takeshi, Kusumi , Ichiro, Takebayashi , Minoru Tricyclic antidepressant amitriptyline indirectly increases the proliferation of adult dentate gyrus-derived neural precursors: an involvement of astrocytes. PLoS ONE, 2013-11-18;8(11):e79371. 2013-11-18 [PMID: 24260208] (Neutralization, Rat)
• Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Brain, 2010-07-21;133(0):2232-47. 2010-07-21 [PMID: 20659956] (Neutralization, Human)
• Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL Dedicated epithelial recipient cells determine pigmentation patterns. Cell, 2007-09-07;130(5):932-42. 2007-09-07 [PMID: 17803914] (Neutralization, Mouse)

FAQs for FGF basic/FGF2/bFGF Antibody (AB-233-NA). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2