FGF basic/FGF2/bFGF Antibody (10060) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 10060
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGF basic/FGF2/bFGF Antibody (10060) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FGF basic/FGF2/bFGF
• Specificity: Recognizes human and bovine FGF basic/FGF2/bFGF. In capture ELISAs, shows approximately 0.2% cross-reactivity with bovine FGF acidic, and no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF-4, rhFGF-6, or rhFGF-7.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human FGF basic Biotinylated Antibody (Catalog number BAM233)
  Standard: Recombinant Human FGF basic 146 aa (Catalog number 233-FB)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF basic/FGF2/bFGF Antibody (10060) [Unconjugated]

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic, also known as FGF2, is a member of the FGF family of growth factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF basic/FGF2/bFGF Antibody (MAB233)(5)

Publications using MAB233

• J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (ELISA Capture, Human)
• S Shamdani, S Chantepie, C Flageollet, N Henni-Cheb, Y Jouan, F Eymard, E Hay, M Cohen-Sola, D Papy-Garci, X Chevalier, P Albanese Heparan sulfate functions are altered in the osteoarthritic cartilage Arthritis Research & Therapy, 2020-12-07;22(1):283. 2020-12-07 [PMID: 33287871] (ELISA Capture, Human)
• MEIQUN SUN, HONGLI LIU, SHENGPING MIN, HONGTAO WANG, XIAOJING WANG Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons Biomedical Reports 4/1/2016 [PMID: 27073624]
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension. Am. J. Physiol. Heart Circ. Physiol., 2005-10-07;290(3):H978-84. 2005-10-07 [PMID: 16214846] (IHC-Fr, Porcine)

FAQs for FGF basic/FGF2/bFGF Antibody (MAB233). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2