FGF basic/FGF2/bFGF Antibody

Product Discontinued

FGF basic/FGF2/bFGF Antibody Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Secreted
• Specificity: Does not cross react with recombinant mouse and human IL6, recombinant human IL1 alpha and IL1 beta, recombinant human TNF alpha, recombinant human IL3, and recombinant human FGF acidic.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGF2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Radioimmunodiffusion
  • Western Blot 1:100-1:2000
• Application Notes: Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. RIA Characterization: The antiserum is characterized utilizing a second antibody-polyethylene glycol (PEG) RIA protocol, where 0.1 ml of a minimum 1/10000 dilution of antiserum has been found to bind at least 40% of 100 picograms of iodinated recombinant human bFGF. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = minimum 1.0 x 109 L/mole.An minimum antibody concentration of 5 g/ml neutralizes 50% of the biological activity of 1 unit of bFGF using the mouse fibroblast cell line 3T3.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Alternate Names for FGF basic/FGF2/bFGF Antibody

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FGF basic/FGF2/bFGF Antibody (NB120-10420). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2
• Entrez:
  • 2247(Human)
  • 14173(Mouse)