FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

Western Blot: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody (M02), clone 2E12.Lane 1: FGF1 transfected lysate(17.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Immunoprecipitation: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 transfected lysate using anti-FGF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGF1 MaxPab rabbit polyclonal antibody.

ELISA: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Detection limit for recombinant GST tagged FGF1 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu, Pl
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FGF1 (AAH32697, 46 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
• Specificity: fibroblast growth factor 1 (acidic)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

• AFGF
• alpha
• alpha-ECGF
• beta-ECGF
• ECGF
• ECGFB
• ECGF-betaAcidic fibroblast growth factor
• endothelial cell growth factor, beta
• FGF acidic
• FGF-1
• FGFABeta-endothelial cell growth factor
• FGF-alpha
• fibroblast growth factor 1 (acidic)
• GLIO703
• HBGF1
• HBGF-1
• heparin-binding growth factor 1

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF acidic/FGF1 Antibody (H00002246-M02)(4)

Publications using H00002246-M02

• Masaaki Y, Shin A, Ronald O et al. Neurovascular unit pathology is observed very early in disease progression in the mutant SOD1 G93A mouse model of amyotrophic lateral sclerosis. Exp Neurol. 2022-04-16 [PMID: 35439439]
• Li J, Chen H, Li X et al. Co-expression of podoplanin and fibroblast growth factor 1 predicts poor prognosis in patients with lung squamous cell carcinoma. Mol Med Rep 2017-06-21 [PMID: 28656229]
• Matsuo K, Matsumura T. Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins. J Biosci Bioeng 2017-03-20 [PMID: 28336416]
• Matsuo K, Fukuzawa N, Matsumura T. A simple agroinfiltration method for transient gene expression in plant leaf discs. J Biosci Bioeng 2016-09-01 [PMID: 26995064]

Bioinformatics

• Gene Symbol: FGF1
• Entrez:
  • 2246(Human)
• OMIM:
  • 131220(Human)
• Uniprot:
  • AAH32697(Human)