Recombinant Human FGF-8 Animal-Free Protein

Images

 
SDS-Page: Recombinant Human FGF-8 Animal-Free Protein [NBP2-76249] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human FGF-8 Animal-Free Protein [NBP2-76249] - FGF-8 induced proliferation of 3T3 cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Human FGF-8 Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Human FGF-8

Source: E. coli

Amino Acid Sequence: MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRGAETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRFEFLNYPPFTR SLRGSQRTWA PEPR

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
FGF-8 is a monomer, 22.5 kDa (194 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
3T3 cell proliferation, >6.7 x 10^5 units/mg (ED50 acceptance criteria are <150 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF8
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 50mM soldium chloride, pH 7.5
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human FGF-8 Animal-Free Protein

  • AIGF
  • AIGFKAL6
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-1287
Species: Hu, Mu, Rt, Rb
Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
NBP2-00932
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF1979
Species: Hu
Applications: WB, Simple Western, IHC, ICC
H00005076-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-51575
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-24715
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-P
NB100-55393
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB319
Species: Hu
Applications: WB, IHC
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
NBP2-57757
Species: Hu
Applications: IHC, IHC-P
NBP2-76249
Species: Hu
Applications: PAGE, Bioactivity

Publications for FGF-8 Recombinant Protein (NBP2-76249) (0)

There are no publications for FGF-8 Recombinant Protein (NBP2-76249).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FGF-8 Recombinant Protein (NBP2-76249) (0)

There are no reviews for FGF-8 Recombinant Protein (NBP2-76249). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF-8 Recombinant Protein (NBP2-76249) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional FGF-8 Products

Bioinformatics Tool for FGF-8 Recombinant Protein (NBP2-76249)

Discover related pathways, diseases and genes to FGF-8 Recombinant Protein (NBP2-76249). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF-8 Recombinant Protein (NBP2-76249)

Discover more about diseases related to FGF-8 Recombinant Protein (NBP2-76249).
 

Pathways for FGF-8 Recombinant Protein (NBP2-76249)

View related products by pathway.

PTMs for FGF-8 Recombinant Protein (NBP2-76249)

Learn more about PTMs related to FGF-8 Recombinant Protein (NBP2-76249).
 

Research Areas for FGF-8 Recombinant Protein (NBP2-76249)

Find related products by research area.

Blogs on FGF-8

There are no specific blogs for FGF-8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human FGF-8 Animal-Free Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF8