Recombinant Human FGF-8 Animal-Free Protein


SDS-Page: Recombinant Human FGF-8 Animal-Free Protein [NBP2-76249] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human FGF-8 Animal-Free Protein [NBP2-76249] - FGF-8 induced proliferation of 3T3 cells.

Product Details

Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Animal Free

Order Details

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Recombinant Human FGF-8 Animal-Free Protein Summary

An un-tagged bioactive recombinant protein corresponding to Human FGF-8

Source: E. coli


Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
FGF-8 is a monomer, 22.5 kDa (194 aa)
Protein is derived from genetically modified E. coli.
Details of Functionality
3T3 cell proliferation, >6.7 x 10^5 units/mg (ED50 acceptance criteria are <150 ng/mL).
E. coli
Protein/Peptide Type
Recombinant Protein
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).


Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 50mM soldium chloride, pH 7.5
No Preservative
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.


Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human FGF-8 Animal-Free Protein

  • AIGF
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Gene Symbol FGF8