FGF-10 Antibody [Alexa Fluor™ Plus 680]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 680

FGF-10 Antibody [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects mouse and human FGF-10 in Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat FGF‑10 is observed and less than 10% cross-reactivity with recombinant mouse FGF-6 and recombinant mouse FGF-7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-10 Antibody [Alexa Fluor™ Plus 680]

• FGF10
• FGF-10
• fibroblast growth factor 10
• Keratinocyte growth factor 2
• KGF2
• KGF-2
• produced by fibroblasts of urinary bladder lamina propria

Background

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62‑209), it shares  94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11‑14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12‑14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).

1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
2. Bellusci, S. et al. (1997) Development 124:4867.
3. Tagashira, S. et al. (1997) Gene 197:399.
4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
6. Min, H. et al. (1998) Genes Dev. 12:3156.
7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
8. Weaver, M. et al. (2000) Development 127:2695.
9. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
10. Sakaue, H. et al. (2002) Genes Dev. 16:908.
11. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
12. Mailleux, A.A. et al. (2002) Development 129:53.
13. Makarenkova, H.P. et al. (2000) Development 127:2563.
14. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
15. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.