Fetuin B Antibody (212607) [Unconjugated]

Western blot shows human serum. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 62 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA(Cap)
• Clone: 212607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin B Antibody (212607) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fetuin B
  Met19-Pro382
  Accession # Q9UGM5
• Specificity: Detects human Fetuin B in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhCystatin A, B, C, rhMMP-2, 9, rhBMP-2, 4, 6, rhTGF-beta 1, or rhTGF-beta 2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FETUB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Simple Western 50 ug/mL
  • Western Blot 5 ug/mL
• Application Notes: ELISA Capture: Human Fetuin B Antibody (Catalog # MAB17251)
  ELISA Detection: Human Fetuin B Biotinylated Antibody (Catalog # BAM17251)
  Standard: Recombinant Human Fetuin B (Catalog # 1725-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin B Antibody (212607) [Unconjugated]

• FETUB
• Fetuin B
• fetuin beta;fetuin phosphoprotein
• fetuin-B
• Fetuin-like protein IRL685,16G2
• Gugu
• IRL685

Background

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

1. Oliver, E., et al. (1999) Genomics. 57:352.
2. Oliver, E., et al. (2000) Biochem. J. 350:589.
3. Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
4. Denecke, B. et al. (2003) Biochem. J. 376:135.
5. Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
6. Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
7. Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FETUB
• Uniprot:
  • Q9UGM5()