Human Fetuin A/AHSG ELISA Kit (Colorimetric)

Product Discontinued

Human Fetuin A/AHSG ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 6.25 to 400 ng/mL
• Sensitivity: 5 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.20%
• Intra-Assay: CV% < 4.80%
• Spike Recovery: 100.50%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AHSG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: AHSG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human AHSG.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Fetuin A/AHSG ELISA Kit (Colorimetric)

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Human fetuin (2-Heremans-Schmid-glycoprotein or a2-HS glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver. Human fetuin is named after its bovine homolog. Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein; normal circulating levels in adults (300600 g/ml) fall significantly (3050%) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone. The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion. Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types. Fetuin plays a role as an anti-inflammatory agent by suppressing the release of TNF from stimulated macrophages. Fetuin also interacts with members of the matrix metalloprotease family of zinc dependent secreted transmembrane proteins that degrade basement membranes and extracellular matrix components. The biological activity of fetuin is mediated through its direct interaction with other proteins. Fetuin down-regulates a number of receptor tyrosine kinase family members. A motif within fetuin has homology to the TGF-b receptor type II. Circulating human plasma fetuin is partly phosphorylated which implies that phosphorylated fetuin may have a physiological function in vivo. Human fetuin isolated from plasma is a two-chain molecule consisting of a A-chain of 322 amino acid residues (53 kDa) and a B-chain of 27 residues (5 kDa). The carboxy terminal 40 amino acids of the A chain constitute a bridging peptide that may be removed proteolytically in vivo. A single mRNA encodes both the A and B chains. The apparent molecular weight of intact human fetuin is 58 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Fetuin A/AHSG ELISA Kit (KA0478)(4)

Publications using KA0478

• Oncu K, Yazgan Y, Tanoglu A et al. Can Serum Fetuin-A Be Regarded as an Inflammatory Marker Among Patients with Familial Mediterranean Fever? Dig Dis Sci 2013-08-08 [PMID: 23925820]
• Merx MW, Schafer C, Westenfeld R et al. Myocardial stiffness, cardiac remodeling, and diastolic dysfunction in calcification-prone fetuin-A-deficient mice. J Am Soc Nephrol 2005-09-21 [PMID: 16177000]
• Geroldi D, Minoretti P, Bianchi M et al. Genetic association of alpha2-Heremans-Schmid glycoprotein polymorphism with late-onset Alzheimer's disease in Italians. Neurosci Lett 2005-10-07 [PMID: 16002217]
• Yang F, Schwartz Z, Swain LD et al. Alpha 2-HS-glycoprotein: expression in chondrocytes and augmentation of alkaline phosphatase and phospholipase A2 activity. Bone 1991-01-01 [PMID: 2054237]

FAQs for Fetuin A/AHSG ELISA Kit (KA0478). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AHSG