Ferritin Light Chain Antibody (JM10-37)

Western Blot: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of Ferritin Light Chain on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. APPLICATIONS

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded mouse testes tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human brain tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human liver tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded mouse kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: JM10-37
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ferritin Light Chain Antibody (JM10-37) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Ferritin Light Chain aa 126-175 / 175. (SwissProt: P02792 Human; SwissProt: P29391 Mouse; SwissProt: P02793 Rat)
• Localization: Cytosol, extracellular exosome, extracellular region, autolysosome, azurophil granule lumen, cytoplasm, intracellular ferritin complex, membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FTL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ferritin Light Chain Antibody (JM10-37)

• Ferritin L subunit
• ferritin L-chain
• ferritin light chain
• ferritin light polypeptide-like 3
• ferritin, light polypeptide
• MGC71996
• NBIA3

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FTL