Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Human intestine lysate.
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Mouse ferroportin, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed for FPN1 (NBP1-21502; 1:1000 dilution) and GAPDH (loading ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with NBP1-21502 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with ...read more
Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin 1 at 5.0 ug/ml ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of FPN1 / SLC40A1 protein in murine small intestinal section using Ferroportin 1 antibody at a dilution of 1:200. The antibody ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Bovine adult eye. Image from verified customer review.
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of FPN1 / SLC40A1 protein in murine liver section using Ferroportin 1 antibody at a dilution of 1:200. The representative image shows ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of NBP1-21502. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF647 (blue) and a matched isotype control (orange, ...read more
62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (PMID: 24895335).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Antibody tested in Flow Cytometry by verified customer through our Innovator's Reward Program.
Alternate Names for Ferroportin/SLC40A1 Antibody
Iron-regulated transporter 1
putative ferroportin 1 variant IIIB
SLC11A3iron regulated gene 1
solute carrier family 11 (proton-coupled divalent metal ion transporters)
solute carrier family 40 (iron-regulated transporter), member 1
solute carrier family 40 member 1
Ferroportin 1 (FPN1 or SLC40A1) is an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen etc.) that play key role in intestinal iron absorption as well as cellular iron release, and mediates iron efflux in the presence of ferroxidases hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in the iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. FPN1 can be regulated transcriptionally, post-transcriptionally, through mRNA stability and post-translationally through protein turnover and its regulation is critical for iron homeostasis because alterations in the same may lead to either iron deficiency or iron overload. FPN1 transports iron in ferrous form whereas plasma transferrin only binds iron's ferric form, and ferroxidases are key players in oxidizing iron transported by FPN1, and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types in the body utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1. Defects in FPN1 have been linked to hemochromatosis type 4 (HFE4), an autosomal dominant iron-loading disorder.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 3 of 3 FAQs).
I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
Would it be possible to conjugate this antibody to PE or PerCP?
With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
The immunogen used for our Ferroportin antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.