Ferroportin/SLC40A1 Antibody - BSA Free

Mouse Ferroportin/SLC40A1, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. Proteins of subcellular fractions isolated from livers of wt-, ms-, and LRS-treated mice (n = 4) were run on a western blot (30 ug protein/lane) and immunostained for microsomal ferroportin. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2014.00079/abstract), licensed under a CC-BY license.

HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with Ferroportin/SLC40A1 Antibody at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Detection of Ferroportin/SLC40A1 protein in murine small intestinal section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The antibody primarily developed a membranous staining pattern in the intestinal epithelial cells.

An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 Antibody NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western blot of human intestine lysate (Molecular weight: 62.5 KDa) using Ferroportin/SLC40A1 Antibody.

Western blot of bovine adult eye using Ferroportin/SLC40A1 Antibody. Image from verified customer review.

Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed using (NBP1-21502; 1:1000 dilution) and GAPDH (loading control). Image from verified customer review.

HepG2 clones overexpressing heparanase showed a reduction of hepcidin expression and indices of iron loading. Two stable clones of HepG2 cells transfected with pcDNA3.1-HPA (HPA3 and HPA6) were analyzed for hepcidin expression, BMP/SMAD signaling and indices of iron status. Western blot of Ferroportin/SLC40A1 and its densitometry. The values are expressed as-dCt (for HPA mRNA) or as fold change over the control (MOCK) (for hepcidin and Id1 mRNA). The images are representative from three different analyses. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371:journal.pone.0164183), licensed under a CC-BY license.

Immunostaining for Ferroportin/SLC40A1 shows uniform reactivity on the ciliary epithelium (panel 1). Fpn is expressed mainly in NPE cells (panel 2). H&E stained section shows orientation of the ciliary epithelium with respect to the iris and AH (panel 3). Fpn is expressed on corneal epithelium (panel 4) and endothelium (panel 5). Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29859760/) licensed under a CC-BY license.

Detection of Ferroportin/SLC40A1 protein in murine liver section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The representative image shows intense staining in the cellular membranes, whereas, a relatively milder postivity was observed in the cytoplasm of hepatocytes.

Analysis using the PE conjugate of Ferroportin/SLC40A1 Antibody. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin/SLC40A1 antibody. Image from verified customer review.

An intracellular stain was performed on HepG2 cells with NBP1-21502AF647 (blue) and a matched isotype control (orange, NBP2-24893AF647). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

An intracellular stain was performed on Hek293 cells with NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Mouse ferroportin, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. Proteins of subcellular fractions isolated from livers of wt-, ms-, and LRS-treated mice (n = 4) were run on a western blot (30 ug protein/lane) and immunostained for (A) microsomal ferroportin.

Immunohistochemistry-Paraffin: Rabbit Polyclonal Ferroportin/SLC40A1 Antibody [NBP1-21502] - Staining of mouse small intestines using Ferroportin/SLC40A1 Antibody. Tissue was fixed in formalin overnight and embedded in paraffin. ~5 micro sections on slides were dehydrated. Antigen retrieval was done using citrate buffer in rice steamer for 20mins. 1 hour blocking without permeabilization. Overnight primary ab incubation was done 1:200 (NBP1-21502) at 4C. 2-hr secondary antibody incubation was at room temp; mounting media with DAPI. Image from a verified customer review.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Analysis of Cu & Fe content & metal-binding protein expression in wild-type & PrPC-null mouse total brain at different ages. (A) The graph shows the ratio of Cu & Fe levels in Prnp0/0 & Prnp+/+ brain samples (P1, P365 N = 4; P7, P30, P180 N = 6; P90 N = 5). (B) Representative Western blot images showing metal-binding protein levels in Prnp0/0 & Prnp+/+ brain samples (N = 4). The constant level of the housekeeping proteins ( beta -III Tubulin & beta -Actin) are also reported. (C) The graph shows the up- or down-regulation of protein expression in Prnp0/0 samples compared to Prnp+/+, i.e., (Prnp0/0 protein OD/housekeeping OD)/(Prnp+/+ protein OD/housekeeping OD). All error bars indicate SD; N = 4 minimum; *p 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28864822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - SHP abrogates the BMP6 effect on iron metabolism through inhibition of hepcidin gene expression in mice.(a–d) C57/BL6 mice were injected with Ad-GFP (n = 4 per group, 5.9 × 109 pfu) or Ad-Flag-SHP (n = 5 per group, 5.9 × 109 pfu) via the tail-vein, & treated with Vehicle or BMP6 (500 μg/kg, i.p.) for 6 h at day 5 after the infection. (a) Serum iron level. (b) Q-PCR analysis showing hepcidin & SHP mRNA levels in liver. (c) Serum hepcidin levels. (d) Western blot analysis showing SMAD1/5/8 phosphorylation & SHP expression in liver. (e) Western blot analysis showing FPN expression in spleen (top). Graphical representation showing FPN expression (bottom). Data are presented as means ± SD. Arrows show locations of molecular weight markers. The western blot images were cropped with a grey cropping line. All gels for western blot analysis were run under the same experimental conditions. **P < 0.01, ***P < 0.001 by two-tailed Student t-test. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep34630), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Upregulation of H3K4me3 increases iron exporter Fpn1 & exhibits neuroprotection.a, b CPI-455 treatment (24 h) increased the protein level of H3K4me3 & Fpn1 in SH-SY5Y cells. c MTT assay showed CPI-455 showed neuroprotection against the toxicity of 6-OHDA in SH-SY5Y cells. d–g SH-SY5Y cells were infected by AAV-KDM5C-silencing virus, & KDM5C was significantly decreased at the mRNA level as reflected by RT-PCR d & at the protein level by Western blot e, f. In addition, Fpn1 & H3K4me3 were increased in AAV-KDM5C-silencing virus infected cells e, f. Moreover, g silencing KDM5C showed a slight neuroprotection against 6-OHDA-induced PD model in SH-SY5Y cells. *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as mean ± SEM. n = 3–4 for each group in Western blot & n = 5 for each group in MTT assay. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33116116), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Upregulation of H3K4me3 increases iron exporter Fpn1 & exhibits neuroprotection.a, b CPI-455 treatment (24 h) increased the protein level of H3K4me3 & Fpn1 in SH-SY5Y cells. c MTT assay showed CPI-455 showed neuroprotection against the toxicity of 6-OHDA in SH-SY5Y cells. d–g SH-SY5Y cells were infected by AAV-KDM5C-silencing virus, & KDM5C was significantly decreased at the mRNA level as reflected by RT-PCR d & at the protein level by Western blot e, f. In addition, Fpn1 & H3K4me3 were increased in AAV-KDM5C-silencing virus infected cells e, f. Moreover, g silencing KDM5C showed a slight neuroprotection against 6-OHDA-induced PD model in SH-SY5Y cells. *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as mean ± SEM. n = 3–4 for each group in Western blot & n = 5 for each group in MTT assay. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33116116), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - SHP deficiency alters hepcidin gene expression in liver of HID mice.(a–i) WT & SHP KO mice (n = 5 per group) were fed with high-iron diet (HID, 8 g/kg) for 3 weeks. (a) Serum iron level. (b) Hepcidin mRNA level in liver. (c) Hepcidin expression in mouse liver. IHC was performed using an antibody against hepcidin. Scale bar shows 50 μm. (d) Serum hepcidin level. (e) Western blot analysis (left panel) showing hepatic SHP & splenic FPN expression & graphical representation (right panel) showing splenic FPN expression. (f) Splenic iron level. (g) Perls’ prussian blue staining in spleen. Scale bar shows 200 μm. (h) BMP6 & BMP9 mRNA levels in liver. (i) Western blot analysis (top) & graphical representation (bottom) showing SMAD1/5/8 phosphorylation in liver. The grouping of the images is from different parts of the same gel. Data are presented as means ± SD. Arrows show locations of molecular weight markers. The experiment was repeated on a minimum of three separate occasions. The western blot images were cropped with a grey cropping line. All gels for western blot analysis were run under the same experimental conditions. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student t-test. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep34630), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Differential expression of proteins of iron metabolism in CCA cells. The CCA4, CCLP1 & HUCCT1 human CCA-derived cell lines were cultured as adherent monolayers (MON) or in 3D sphere conditions (SPH). Panel a. Top left, representative immunoblot analysis. Cell extracts were reacted with antibodies against transferrin receptor (TfR1), ferroportin (FPN), ferritin H subunit (FtH) & vinculin. Cropped blots are displayed. The original full blot images can be found in Supplementary Information. The graphs show densitometric quantification of immunoblot analyses. The values were normalized to vinculin & expressed as a fraction of respective MON cells normalized to 1. Mean values ± SEM (n = 6), *p ≤ 0.05, **p ≤ 0.01 vs control MON for each cell line. Panel b. RNA bandshift analysis of IRP activity. Cytoplasmic extracts were incubated with a 32P-labeled iron-responsive element (IRE) probe & RNA-protein complexes separated on non-denaturing polyacrylamide gels. On the left a representative autoradiogram is shown. A cropped gel is displayed. The original full gel image can be found in Supplementary Information. The graph on the right shows the densitometric quantification of IRPs bands by direct nuclear counting, as described in Materials & Methods; mean percentages ± SEM of control values (n = 6),, **p ≤ 0.01, ***p< 0.001 vs control MON for each cell line. Panel c. TfR1 & FPN mRNA levels were measured by quantitative RT-PCR. Samples were analyzed in triplicate, normalized to the housekeeping gene 18 S & expressed as percentage of respective MON cells normalized to 1. Mean values ± SEM (n = 6), **p ≤ 0.01 vs control MON for each cell line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29247214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Iron (55Fe) transport across the apical & basal borders. (a) Apical transport (uptake) of 55Fe in response to intracellular/basal ascorbate (150 μM) was increased compared to no ascorbate. The cells were incubated with ascorbate in the basal chamber for 24 h. Values are means of 10 samples ±SD. The difference between treatments was significant (p = 0.04). (b) Basolateral transport of iron (as 55Fe) in response to intracellular/basal ascorbate (150 μM) was increased compared to no ascorbate. The cells were incubated with ascorbate in the basal chamber for 24 h. Values are means of 10 samples ±SD. The difference between treatments was significant (p = 0.03). (c) Western Blot of cells treated with ascorbate (150 μM). Lane 1: Control cells (no treatment). Lane 2: Basal ascorbate at 150 μM; these were the ferroportin levels before iron addition in (a). Lane 3: Ferroportin levels 22 h after the iron addition (20 μM for 2 h) in (a). Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/6/1/249), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Metformin rescues BMP6-mediated alteration of iron metabolism in mice.(a–e) C57/BL6 mice (n = 4 per group) were treated with BMP6 (500 μg/kg, i.p.) & metformin (200 mg/kg, p.o.). (a) Serum iron level. (b) Q-PCR analysis showing hepcidin & SHP mRNA levels in liver. (c) Serum hepcidin levels. (d) Western blot analysis showing SMAD1/5/8 phosphorylation & FPN in liver. (e) Western blot analysis (top) & graphical representation (bottom) showing FPN expression in spleen. (f) Schematic diagram of SHP-mediated inhibition of BMP6-SMADs pathway. Data are presented as means ± SD. Arrows show locations of molecular weight markers. The western blot images were cropped with a grey cropping line. All gels for western blot analysis were run under the same experimental conditions. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student t-test. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep34630), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Comparison of Cu, Fe, & metal-binding protein expression levels in wild-type & PrPC-null mouse spleen at different ages. (A) The graph shows the ratio of Cu & Fe levels in Prnp0/0 & Prnp+/+ spleen samples (P15 N = 3; P30 N = 4; P90, P180 N = 6; P365 N = 5). (B) The graph shows the weight of spleen extracted from Prnp0/0 & Prnp+/+ mice; N = 4. (C) Representative Western blot images showing metal-binding protein levels in Prnp0/0 & Prnp+/+ spleen samples. The constant level of the housekeeping protein ( beta -Actin) are also reported. (D) The graph shows the up- or down-regulation of protein expression in Prnp0/0 samples compared to Prnp+/+, i.e., (Prnp0/0 protein OD/housekeeping OD)/ (Prnp+/+ protein OD/housekeeping OD); N = 4. All error bars indicate SD; *p < 0.05; **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27729845), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Htt elimination disrupts brain iron homeostasis.(A) Representative photographs of immunohistochemical staining for ferritin light chain (Ft). Left panels: cerebella of 21mo CTL noTM & 18mo cKO TM@3mo; middle panels: thalami of 12mo CTL TM@9mo & 12mo cKO TM@9mo; right panels: striata of 21mo CTL noTM & 18mo cKO TM@3mo. Note that Ft levels are already extremely reduced in the thalamus 3 months after Htt elimination (middle panels), & that Ft expression is practically abolished in the cerebellum in the absence of Htt (left panels). (B) Representative western blots of Tfr & Fpn protein expression in 10 month-old brains from CTL & cKO mice TM-treated at 6 months of age. Antibody against actin was used as internal control for loading. Note that Tfr & Fpn levels are increased in TM-treated cKO compared to controls. (C) Quantification of Tfr & Fpn expression levels. Western blots of total protein extracts from 10 month-old brains CTL (n = 5) & cKO (n = 5) TM-treated at 6 months of age were probed with anti-Tfr or anti-Fpn, stripped & re-probed with anti-actin antibody. Bands intensities were quantitated using Image J. Tfr & Fpn levels were normalized over actin levels. Values represent mean relative to controls ± SD (***P<0.001, Student’s t-test). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - GW501516 activation of PPARδ regulates 6-OHDA-triggered expression of level of DMT1 & FPN1 protein, & DMT1 mRNA, but not FPN1 mRNA. (A) Cells were treated with 6-OHDA for the indicated durations. (B,C) Cells pretreated with DMSO or GW501516 for 8 h were incubated with or without 6-ODHA for 16 h. (D) Cells were treated with GW501516 for the indicated durations. (E,F) Cells pretreated with DMSO or GW501516 for 8 h were incubated with or without 6-ODHA for 16 h. Total RNA & protein were extracted, & mRNA & protein levels were analyzed by real-time PCR (A,B,D,E) & Western blot (C,F), respectively. RPS18 & alpha -tubulin were used as internal controls for real-time PCR & Western blot, respectively. Results are expressed as means ± SE (n = 3). An image analyzer was used to quantify band intensity of Western blot, & the ratio of protein to alpha -tubulin is indicated above each lane. * p < 0.05, ** p < 0.01 relative to the untreated group; #p < 0.05, ##p < 0.01 relative to the 6-OHDA-treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624674), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Fisetin protects against DOX-induced ferroptosis via regulating Nrf2 in rats. (A). Western blot results of Nrf2 & Keap1, HO-1, FTH1, FTL, FPN, & TfR1 protein in control & different drug-treated rat heart tissues. (B). Quantification of (A). (C). Expression of Nrf2 was detected by immunohistochemistry (IHC) (Representative images, 200X & 400X, Scale bar = 100 & 50 μm, n = 6 rats per groups) in cardiac tissue of each group. The values are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; NS, no significance; DOX, doxorubicin. Nrf2, nuclear factor erythroid 2-related factor 2; Keap1, Kelch-like ECH-associated protein 1; HO-1, heme oxygenase-1; FTH1, ferritin heavy chain 1; FTL, ferritin light chain; FPN, ferroportin; TfR1, transferrin receptor 1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35273493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Distribution of TfR & Fpn in the anterior segment of bovine eye:(A) The reaction for TfR is evident on the ciliary epithelium facing the AH (panel 1). TfR is localized on the apical membrane & intracellular vesicles of NPE cells as in the human sample (panel 2). The corneal epithelium does not react for TfR (panel 3), while the corneal endothelium shows a distinct reaction on the plasma membrane (panel 4). H&E staining shows multiple layers of corneal epithelium, the stroma, & a single layer of corneal endothelium (panel 5). Scale bar: 25 μm. (B) Fpn is expressed on the ciliary epithelium & the iris (panel 1). The expression of Fpn is limited to NPE cells as in the human sample (panel 2). H&E staining shows the orientation of ciliary epithelium with respect to the iris & AH (panel 3). Unlike human samples, the bovine corneal epithelium did not react for Fpn (panel 4). However, corneal endothelium showed strong reactivity for Fpn (panel 5). Scale bar: 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29859760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - GW501516 PPARδ activation suppresses 6-OHDA-induced expression of IRP1 in SH-SY5Y cells. (A,B) Cells were treated with 6-OHDA (A) or GW501516 (B) for the indicated durations. (C) Cells stably expressing shRNA targeting scrambled sequences or PPARδ were pretreated with DMSO or GW501516 for 8 h & subsequently incubated in the presence or absence of 6-ODHA for 16 h. (D,E) Cells pretreated with DMSO or GW501516 for 8 h were incubated with or without 6-ODHA for 16 h. Total RNA & protein were extracted, & levels of mRNA & protein were analyzed by real-time PCR (A–D) & Western blot (E). The immunoblots are separate from those shown in Figure 4C,F. RPS18 & alpha -tubulin were used as internal controls for real-time PCR & Western blot, respectively. Results are expressed as means of triplicate ± SE (A–D). * p < 0.05, ** p < 0.01 relative to untreated group; #p < 0.05 relative to 6-OHDA-treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624674), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Expression of iron modulating proteins in the cornea & ciliary body.(A) Probing of lysates from bovine cornea (Cor) & CB for Cp, TfR, Tf, & ferritin shows the expression of all of these proteins in both samples (lanes 1–4). Deglycosylation results in faster migration of Cp, TfR, & Tf on SDS-PAGE, indicating the presence of glycans (lanes 2 & 4). AH & the vitreous show abundant presence of Cp & Tf, both of which migrate faster upon deglycosylation (lanes 7 & 9). No reactivity for TfR or ferritin is detected in these samples (lanes 6–9). Human brain lysate was processed in parallel as a positive control (lane 5). Gapdh served as a loading control. (Cor: cornea; Ft: ferritin). (B) Relative distribution of iron modulating proteins within each tissue shows higher expression of TfR relative to Cp & ferritin in the cornea, & higher levels of Cp relative to the TfR & ferritin in the CB. (C) Quantitative comparison of protein expression by densitometry shows significantly higher levels of ferritin & Cp, & lower levels of TfR in the CB relative to the cornea. All values were normalized to Gapdh that provided the loading control. Values represent fold change ± SEM of the indicated n. (D) Probing of Western blots of bovine cornea & CB for Fpn revealed increased expression of Fpn (3.2 fold) in CB relative to the cornea (lanes 1–4). Lysates from human brain & bovine retina were analyzed in parallel as controls (lanes 5 & 6). Gapdh served as a loading control. (E) Quantification by densitometry shows 3.2 fold higher levels of Fpn in the CB relative to the cornea. Values are mean + SEM of the indicated n. **p < 0.01. The full images of the cropped blots have been provided in the Supplementary Data. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29859760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ferroportin/SLC40A1 Antibody - BSA Free Summary

• Immunogen: This Ferroportin/SLC40A1 Antibody was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]
• Localization: Cell membrane (high), Cytoplasm (low)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC40A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1.0 ug/ml. Use reported by customer review
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 ug/ml
• Application Notes: In Western blot, a band is seen at ~62 kDa.
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Ferroportin/SLC40A1 Protein (NBP1-21502PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ferroportin/SLC40A1 Antibody - BSA Free

• Ferroportin
• Ferroportin-1
• FPN1
• FPN1IREG1ferroportin 1
• HFE4
• HFE4ferroportin-1
• IREG1
• iron regulated gene 1
• Iron-regulated transporter 1
• member 3
• MST079
• MSTP079
• MTP1
• putative ferroportin 1 variant IIIB
• SLC11A3
• SLC11A3iron regulated gene 1
• SLC40A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)
• solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
• solute carrier family 40 (iron-regulated transporter), member 1
• solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(191)

Publications using NBP1-21502

• Liu Q, Han Z, Li T et Al. Microglial HO-1 aggravates neuronal ferroptosis via regulating iron metabolism and inflammation in the early stage after intracerebral hemorrhage Int Immunopharmacol 2024-12-30 [PMID: 39740507]
• Abboud, E;Chrayteh, D;Boussetta, N;Dalle, H;Malerba, M;Wu, TD;Le Gall, M;Reelfs, O;Pourzand, C;Mellett, M;Assan, F;Bachelez, H;Poupon, J;Aractingi, S;Vaulont, S;Sohier, P;Oules, B;Karim, Z;Peyssonnaux, C; Skin hepcidin initiates psoriasiform skin inflammation via Fe-driven hyperproliferation and neutrophil recruitment Nature communications 2024-08-07 [PMID: 39112467]
• Horeau M, Delalande M, Ropert M et Al. Sex similarities and divergences in systemic and muscle iron metabolism adaptations to extreme physical inactivity in rats J Cachexia Sarcopenia Muscle 2024-07-24 [PMID: 39049183]
• Shaina L. Rosenblum, Danielle K. Bailey, Daniel J. Kosman Calcium and IL-6 regulate the anterograde trafficking and plasma membrane residence of the iron exporter ferroportin to modulate iron efflux The Journal of Biological Chemistry 2024-05-06 [PMID: 38718866]
• Zhuanzhuan Liu, Hanying Wang, Zhiwei Zhang, Yulu Ma, Qiyue Jing, Shenghai Zhang, Jinzhi Han, Junru Chen, Yaoyao Xiang, Yanbo Kou, Yanxia Wei, Lu Wang, Yugang Wang, Igor C. Almeida Fam96a is essential for the host control of Toxoplasma gondii infection by fine-tuning macrophage polarization via an iron-dependent mechanism PLOS Neglected Tropical Diseases 2024-05-07 [PMID: 38713713]
• Yang WP, Li MQ, Ding J et Al. High-altitude hypoxia exposure inhibits erythrophagocytosis by inducing macrophage ferroptosis in the spleen Elife 2024-04-17 [PMID: 38629942]
• Yifan Sun, Lanlan Tang, Xianjin Kan, Lei Tan, Cuiping Song, Xusheng Qiu, Ying Liao, Venugopal Nair, Chan Ding, Xiufan Liu, Yingjie Sun Oncolytic Newcastle disease virus induced degradation of YAP through E3 ubiquitin ligase PRKN to exacerbate ferroptosis in tumor cells Journal of Virology 2024-03-01 [PMID: 38411946]
• Kang DY, Park S, Song KS et al. Anticancer Effects of 6-Gingerol through Downregulating Iron Transport and PD-L1 Expression in Non-Small Cell Lung Cancer Cells Cells 2023-11-15 [PMID: 37998363] (WB, Human)
• Soofi A, Li V, Beamish JA et al. Renal specific loss of Ferroportin disrupts iron homeostasis and attenuates recovery from acute kidney injury American journal of physiology. Renal physiology 2023-11-23 [PMID: 37994409] (ICC/IF, Mouse)
• Yang W, Li J, Hu J et al. Hypobaric hypoxia induces iron mobilization from liver and spleen and increases serum iron via activation of ghrelin/GHSR1a/MAPK signalling pathway in mice Scientific reports 2023-11-20 [PMID: 37985861] (WB, Mouse)
• Deng GH, Wu CF, Li YJ et al. Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease Military Medical Research 2023-11-08 [PMID: 37941054] (IHC, WB, Mouse)
• Peng R, Liu X, Wang C et al. Iron overload enhances TBI-induced cardiac dysfunction by promoting ferroptosis and cardiac inflammation Biochemical and biophysical research communications 2023-11-19 [PMID: 37801989]
• Pokrajac NT, Tokarew NJA, Gurdita A et al. Meningeal macrophages inhibit chemokine signaling in pre-tumor cells to suppress mouse medulloblastoma initiation Developmental cell 2023-10-23 [PMID: 37774709]
• Yamada K, Tazaki A, Ushio-Watanabe N et al. Retinal ferroptosis as a critical mechanism for the induction of retinochoroiditis during ocular toxoplasmosis Redox Biol 2023-09-17 [PMID: 37738924] (Western Blot, Mouse)
• Li Q, Xu T, Zha X et al. Buddlejasaponin Ivb Ameliorates Ferroptosis of Dopaminergic Neuron by Suppressing Irp2-Mediated Iron Overload in Parkinson's Disease SSRN 2023-07-14 [PMID: 37717841] (WB, Human, Mouse)
• Ma SJ, Li C, Yan C et al. Melatonin alleviates early brain injury by inhibiting the NRF2-mediated ferroptosis pathway after subarachnoid hemorrhage Free radical biology & medicine 2023-09-17 [PMID: 37717795] (WB, Rat)
• Kaur H, Minchella P, Alvarez-Carbonell D et al. Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells Preprints.org 2023-07-24 [PMID: 37569616] (WB, Human)
• Liu W, Zhang S, Li Q et al. Lactate modulates iron metabolism by binding soluble adenylyl cyclase Cell metabolism 2023-07-18 [PMID: 37480842]
• Tamiya H, Urushihara N, Shizuma K et al. SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-?B- and PRMT5-Mediated PGC1? Reduction Cancers (Basel) 2023-07-04 [PMID: 37444594] (Western Blot, Mouse)
• Sun F, Cui L, Qian J et al. Decidual Stromal Cell Ferroptosis Associated with Abnormal Iron Metabolism Is Implicated in the Pathogenesis of Recurrent Pregnancy Loss International Journal of Molecular Sciences 2023-04-25 [PMID: 37175543] (IHC-P, WB, Human)
• Qin Y, Bai D, Tang M et al. Ketogenic diet alleviates brain iron deposition and cognitive dysfunction via Nrf2-mediated ferroptosis pathway in APP/PS1 mouse Brain research 2023-08-01 [PMID: 37164173] (WB, Mouse)
• Shi L, Tao Z, Zheng L et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgf?1 signaling pathway Redox Biology 2023-05-01 [PMID: 37156218] (WB, Mouse)
• Lin Q, Hu DW, Hao XH et al. Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat Brains Neural plasticity 2023-04-20 [PMID: 37124874] (Western Blot, Mouse)
• Mukhopadhyay S, Encarnación-Rosado J, Lin EY et al. Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer Science advances 2023-04-21 [PMID: 37075122] (WB, Mouse)
• Principe P, Mukosera GT, Gray-Hutto N et al. Nitric Oxide Affects Heme Oxygenase-1, Hepcidin, and Transferrin Receptor Expression in the Placenta International journal of molecular sciences 2023-03-20 [PMID: 36982960] (Western Blot, Mouse)
• Bao X, Luo X, Bai X et al. Cigarette tar mediates macrophage ferroptosis in atherosclerosis through the hepcidin/FPN/SLC7A11 signaling pathway Free radical biology & medicine 2023-03-16 [PMID: 36933812] (Western Blot, Mouse)
• Tan X, Zhang T, Ding X et al. Iron overload facilitates neonatal hypoxic-ischemic brain damage via SLC7A11-mediated ferroptosis Journal of neuroscience research 2023-03-16 [PMID: 36929608]
• Wu Y, Huang T, Li X et Al. Retinol dehydrogenase 10 reduction mediated retinol metabolism disorder promotes diabetic cardiomyopathy in male mice Nat Commun 2023-03-02 [PMID: 36864033] (Immunohistochemistry, Western Blot)
• Nagpal A, Needham K, Lane DJR et al. Integrin ?v?3 Is a Master Regulator of Resistance to TKI-Induced Ferroptosis in HER2-Positive Breast Cancer Cancers 2023-02-14 [PMID: 36831558] (WB, Mouse)
• Xie J, Lv H, Liu X et al. Nox4-and Tf/TfR-mediated peroxidation and iron overload exacerbate neuronal ferroptosis after intracerebral hemorrhage: Involvement of EAAT3 dysfunction Free radical biology & medicine 2023-02-18 [PMID: 36805044] (WB, Rat)
• Zhao J, Jia Y, Mahmut D et al. Human hematopoietic stem cell vulnerability to ferroptosis Cell 2023-02-16 [PMID: 36803603]
• Santhakumar S, Athiyarath R, Cherian AG et al. Impact of maternal iron deficiency anemia on fetal iron status and placental iron transporters in human pregnancy Blood cells, molecules & diseases 2023-01-27 [PMID: 36725474] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Han Y, Luo Z, Yue ZG et al. The tendency of anemia of inflammation in periodontal diseases Clinical science (London, England : 1979) 2023-02-14 [PMID: 36705427] (WB, Mouse)
• Belvin BR, Lewis JP. Ferroportin depletes iron needed for cell cycle progression in head and neck squamous cell carcinoma Frontiers in Oncology 2023-01-09 [PMID: 36698390] (Western Blot, Mouse)
• Zahra Masoumi, Lucas R Hansson, Eva Hansson, Evelina Ahlm, Eva Mezey, Lena Erlandsson, Stefan R Hansson Assessing erythroferrone and iron homeostasis in preeclamptic and normotensive pregnancies: A retrospective study. Placenta 2023-02-24 [PMID: 36696784]
• Y Zhang, P Dong, N Liu, JY Yang, HM Wang, Q Geng TRIM6 Reduces Ferroptosis and Chemosensitivity by Targeting SLC1A5 in Lung Cancer Oxidative Medicine and Cellular Longevity, 2023-01-09;2023(0):9808100. 2023-01-09 [PMID: 36654781] (Western Blot, Mouse)
• Joffin N, Gliniak CM, Funcke JB et al. Adipose tissue macrophages exert systemic metabolic control by manipulating local iron concentrations Nature metabolism 2022-11-01 [PMID: 36329217] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Wang H, Dou M, Li J et al. Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation In vitro cellular & developmental biology. Animal 2022-09-30 [PMID: 36178582] (ICC/IF, Rat)
• Song Y, Yang N, Si H et al. Iron overload impairs renal function and is associated with vascular calcification in rat aorta Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2022-09-30 [PMID: 36178540] (ICC/IF, Rat)
• DK Suh, WY Lee, WJ Yeo, BS Kyung, KW Jung, HK Seo, YS Lee, DW Suh A Novel Muscle Atrophy Mechanism: Myocyte Degeneration Due to Intracellular Iron Deprivation Cells, 2022-09-13;11(18):. 2022-09-13 [PMID: 36139428] (Western Blot, Mouse)
• Li L, Wang K, Jia R et al. Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation Redox biology 2022-08-23 [PMID: 36029649] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Wang LJ, Zhao GP, Wang XF et al. Glycochenodeoxycholate Affects Iron Homeostasis via Up-Regulating Hepcidin Expression Nutrients 2022-08-02 [PMID: 35956351] (WB, Human, Mouse)
  
  Details:
  Dilution used 1:1000
• Rossi F, Tortora C, Di Martino M et al. Alteration of osteoclast activity in childhood cancer survivors: Role of iron and of CB2/TRPV1 receptors PloS one 2022-07-21 [PMID: 35862357] (Western Blot, Mouse)
• Doan LH, Chu LW, Huang ZY et al. Virofree, an Herbal Medicine-Based Formula, Interrupts the Viral Infection of Delta and Omicron Variants of SARS-CoV-2 Frontiers in pharmacology 2022-07-04 [PMID: 35860023]
• Yuan B, Zhao XD, Shen JD et al. Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage Oxidative medicine and cellular longevity 2022-07-09 [PMID: 35855863] (WB, Mouse)
• Wang D, Zhang S, Ge X et al. Mesenchymal stromal cell treatment attenuates repetitive mild traumatic brain injury-induced persistent cognitive deficits via suppressing ferroptosis Journal of neuroinflammation 2022-07-14 [PMID: 35836233] (WB, Mouse)
  
  Details:
  1:1000 dilution
• Hu CB, Jiang H, Yang Y et al. DL-3-n-butylphthalide alleviates motor disturbance by suppressing ferroptosis in a rat model of Parkinson's disease Neural Regeneration Research 2023-01-01 [PMID: 35799542] (WB, Rat)
• Santana-Codina N, Del Rey MQ, Kapner KS, Zhang H et Al. NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins Cancer Discov 2022-06-30 [PMID: 35771492]
• Zhang H, Jamieson KL, Grenier J et al. Myocardial Iron Deficiency and Mitochondrial Dysfunction in Advanced Heart Failure in Humans Journal of the American Heart Association 2022-06-07 [PMID: 35656974] (WB, Human)
• Lee WJ, Lee HG, Hur J et al. PPAR? Activation Mitigates 6-OHDA-Induced Neuronal Damage by Regulating Intracellular Iron Levels Antioxidants (Basel) 2022-04-21 [PMID: 35624674] (Western Blot, Mouse)
• Wang C, Zhang W, Xu W et al. AMP-activated protein kinase alpha 1 phosphorylates PHD2 to maintain systemic iron homeostasis Clinical and translational medicine 2022-05-01 [PMID: 35538889] (WB, Mouse)
• César Vásquez-Trincado, Julia Dunn, Ji In Han, Briyanna Hymms, Jaclyn Tamaroff, Monika Patel, Sara Nguyen, Anna Dedio, Kristin Wade, Chinazo Enigwe, Zuzana Nichtova, David R. Lynch, Gyorgy Csordas, Shana E. McCormack, Erin L. Seifert Frataxin deficiency lowers lean mass and triggers the integrated stress response in skeletal muscle JCI Insight 2022-05-09 [PMID: 35531957]
• Toe QK, Issitt T, Mahomed A et al. Human pulmonary artery endothelial cells upregulate ACE2 expression in response to iron-regulatory elements: Potential implications for SARS-CoV-2 infection Pulmonary Circulation 2022-04-08 [PMID: 35514788] (Western Blot, Mouse)
• Jiang H, Zhang X, Yang W et al. Ferrostatin-1 Ameliorates Liver Dysfunction via Reducing Iron in Thioacetamide-induced Acute Liver Injury in Mice Frontiers in Pharmacology 2022-04-12 [PMID: 35496274] (WB, Mouse)
• Park WR, Choi B, Kim YJ et al. Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes International journal of molecular sciences 2022-03-25 [PMID: 35408955] (WB, Mouse)
• Xin Chen, Xinxin Song, Jingbo Li, Ruoxi Zhang, Chunhua Yu, Zhuan Zhou, Jiao Liu, Siyan Liao, Daniel J. Klionsky, Guido Kroemer, Jinbao Liu, Daolin Tang, Rui Kang Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis Autophagy 2022-04-10 [PMID: 35403545]
• Zhang X, Song T, Zhao M et al. Sirtuin 2 Alleviates Chronic Neuropathic Pain by Suppressing Ferroptosis in Rats Frontiers in Pharmacology 2022-03-23 [PMID: 35401208] (Western Blot, Mouse)
• Soliman S, El-Labbad E, Abu-Qiyas A et al. Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis Frontiers in Microbiology 2022-03-21 [PMID: 35387075] (WB, Human)
• Li D, Liu X, Pi W et al. Fisetin Attenuates Doxorubicin-Induced Cardiomyopathy In Vivo and In Vitro by Inhibiting Ferroptosis Through SIRT1/Nrf2 Signaling Pathway Activation Frontiers in Pharmacology 2022-02-22 [PMID: 35273493] (Western Blot, Mouse)
• Jiang H, Muir R, Gonciarz R et al. Exploiting KRAS-Driven Ferroaddiction in Cancer Through Ferrous Iron-Activatable Drug Conjugates (FeADC) J Exp Med 2022-03-09 [PMID: 35262628]
• FernAndez-GarcIa V, GonzAlez-Ramos S, AvendaNo-Ortiz J Et al. NOD1 splenic activation confers ferroptosis protection and reduces macrophage recruitment under pro-atherogenic conditions Biomed Pharmacother 2022-03-05 [PMID: 35247718] (FLOW, Mouse)
  
  Details:
  Citation using the PE version of this antibody.
• Wyart E, Hsu MY, Sartori R et al. Iron supplementation is sufficient to rescue skeletal muscle mass and function in cancer cachexia EMBO reports 2022-02-24 [PMID: 35199910] (WB, Human)
• Sato T, Shapiro JS, Chang HC et al. Aging is associated with increased brain iron through cortex-derived hepcidin expression eLife 2022-01-11 [PMID: 35014607] (WB, Mouse)
• Arita A, Kita I, Shinoda S Internalization and Decrease of Duodenal DMT1 Involved in Transient Suppression of Iron Uptake in Short-Acting Mucosal Block Biological trace element research 2022-01-08 [PMID: 34997531]
• Deng Q, Yang S, Sun L et al. A detrimental role of NLRP6 in host iron metabolism during Salmonella infection Redox biology 2021-12-18 [PMID: 34942528] (WB, Mouse)
• Sp N, Kang DY, Jo ES et al. Iron Metabolism as a Potential Mechanism for Inducing TRAIL-Mediated Extrinsic Apoptosis Using Methylsulfonylmethane in Embryonic Cancer Stem Cells Cells 2021-10-22 [PMID: 34831070] (Western Blot, Mouse)
• Sp N, Kang DY, Jo ES Et al. Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells Frontiers in oncology 2021-11-03 [PMID: 34804985] (WB, Human)
• Kang DY, Sp N, Jo ES Et al. New Insights into the Pivotal Role of Iron/Heme Metabolism in TLR4/NF- kappa B Signaling-Mediated Inflammatory Responses in Human Monocytes Cells 2021-09-27 [PMID: 34685529] (WB, Human)
• Han M, BOhlke M, Maher T, Kim J Alcohol exposure increases manganese accumulation in the brain and exacerbates manganese-induced neurotoxicity in mice Archives of toxicology 2021-12-01 [PMID: 34590183] (WB, Mouse)
• Liu K, Liu J, Zou B et al. Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice Cellular and molecular gastroenterology and hepatology 2021-09-22 [PMID: 34562639] (WB, Mouse)
• VAsquez-Trincado C, Patel M, Sivaramakrishnan A et al. Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation Human molecular genetics 2021-09-22 [PMID: 34550363]
• Konieczny P, Mukherjee S, Stepniak-Konieczna E Et al. Cyclic mismatch binding ligands interact with disease-associated CGG trinucleotide repeats in RNA and suppress their translation Nucleic acids research 2021-08-06 [PMID: 34358321] (WB)
• Wang X, Wang Z, Cao J Et Al. Melatonin Alleviates Acute Sleep Deprivation-Induced Memory Loss in Mice by Suppressing Hippocampal Ferroptosis Frontiers in Pharmacology 2021-07-16 [PMID: 34335271] (WB)
• Traeger L, Wiegand SB, Sauer AJ et Al. UBA6 and NDFIP1 regulate the degradation of ferroportin Haematologica 2021-07-29 [PMID: 34320783]
• Bailey Dk, Clark W, Kosman Dj The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells PloS one 2021-07-26 [PMID: 34310628] (WB)
• Zhao S, Wang X, Zheng X Et Al. Iron deficiency exacerbates cisplatin- or rhabdomyolysis-induced acute kidney injury through promoting iron-catalyzed oxidative damage Free radical biology & medicine 2021-07-21 [PMID: 34298093] (WB)
• Argenziano M, Tortora C, Paola AD et al. Eltrombopag and its iron chelating properties in pediatric acute myeloid leukemia Oncotarget 2021-07-06 [PMID: 34262648] (WB)
• Chaudhary S, Ashok A, Wise AS et al. Upregulation of brain hepcidin in prion diseases Prion 2021-12-01 [PMID: 34224321] (WB)
• Wei D, Duan Bx, Wang Cy, Cao P MicroRNA-302a-3p induces ferroptosis of non-small cell lung cancer cells via targeting ferroportin Free radical research 2021-06-28 [PMID: 34181495]
• Zhang Z, Funcke JB, Zi Z et al. Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity Cell metabolism 2021-06-18 [PMID: 34174197]
• Schwartz AJ, Goyert JW, Solanki S et al. Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells Nature metabolism 2021-06-21 [PMID: 34155415]
• Mohammad G, Matakidou A, Robbins PA, Lakhal-Littleton S The kidney hepcidin/ferroportin axis controls iron reabsorption and determines the magnitude of kidney and systemic iron overload Kidney international 2021-05-12 [PMID: 33991530] (IHC-P, WB, Mouse)
• Tang Z, Jiang W, Mao M et al. Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin Clinical and Translational Medicine 2021-05-01 [PMID: 33931967] (Western Blot, Mouse)
• Asperti M, Bellini S, Grillo E et al. H-ferritin suppression and pronounced mitochondrial respiration make Hepatocellular Carcinoma cells sensitive to RSL3-induced ferroptosis Free radical biology & medicine 2021-04-20 [PMID: 33892112]
• Lu S, Song Y, Luo R et al. Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo Oxidative medicine and cellular longevity 2021-02-10 [PMID: 33628376] (WB, IF/IHC)
• Derbis M, Kul E, Niewiadomska D et al. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats Nature communications 2021-02-24 [PMID: 33627639] (WB, Human)
• Deng Q, Yang S, Sun L, et al. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin-ferroportin axis Gut microbes 2021-01-21 [PMID: 33475464] (WB, Mouse)
• Li J, Liu J, Xu Y, et al. Tumor heterogeneity in autophagy-dependent ferroptosis Autophagy 2021-01-15 [PMID: 33404288]
• Li Y, Zeng X, Lu D, et al. Erastin induces ferroptosis via ferroportin-mediated iron accumulation in endometriosis Human reproduction (Oxford, England) 2020-12-30 [PMID: 33378529]
• FernAndez-MendIvil C, Luengo E, Trigo-Alonso P et al. Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism Redox Biol 2020-11-06 [PMID: 33212416] (WB)
• Yamaguchi H, Honda S, Torii S et al. Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration Nat Commun 2020-10-20 [PMID: 33082312] (WB, ICC/IF, Human)
• Verma N, Vinik Y, Saroha A et al. Synthetic lethal combination targeting BET uncovered intrinsic susceptibility of TNBC to ferroptosis Sci. Adv. 2020-08-01 [PMID: 32937365]
• Yang G, Qian C, Zhang C et al. Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats Transl Res 2020-09-12 [PMID: 32932001] (WB, Rat)
• Renassia C, Louis S, Cuvellier S et al. Neutrophils from hereditary hemochromatosis patients are protected from iron excess and are primed Blood Adv 2020-08-25 [PMID: 32810223] (WB, Human)
• Lin PL, Tang HH, Wu SY et al. Saponin Formosanin C-induced Ferritinophagy and Ferroptosis in Human Hepatocellular Carcinoma Cells Antioxidants (Basel) 2020-07-29 [PMID: 32751249] (WB, Human)
• Kim HY, Kwon WY, Park JB et al. Hepatic STAMP2 mediates recombinant FGF21-induced improvement of hepatic iron overload in nonalcoholic fatty liver disease FASEB J. 2020-07-28 [PMID: 32721044]
• Pease NA, Shephard MS, Sertorio M et al. DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages Cancers (Basel) 2020-07-17 [PMID: 32708944] (WB, Mouse)
• Lee J, You JH, Shin D, Roh JL Inhibition of Glutaredoxin 5 predisposes Cisplatin-resistant Head and Neck Cancer Cells to Ferroptosis Theranostics 2020-06-19 [PMID: 32685019] (WB, Human)
• Tsuji Y Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SLC40A1 (ferroportin) PLoS ONE 2020-07-09 [PMID: 32645092] (WB, KD, Human)
• Hazlett HF, Hampton TH, Aridgides DS et al. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival Sci Rep 2020-07-02 [PMID: 32616918]
• Kammerer L, Mohammad G, Wolna M, Robbins P Fetal liver hepcidin supports acquisition of iron stores in utero BioRxiv 2019-10-09 [PMID: 32542311] (IHC-Fr, Mouse)
• KAmmerer, L, Mohammad, G Et al. Fetal liver hepcidin secures iron stores in utero. Blood 2020-09-24 [PMID: 32542311] (WB, Mouse)
• Li S, Qian ZM, Xu G et al. Hepatocyte growth factor protects PC12 cells against OGD/R-induced injury by reducing iron Biosci. Rep. 2020-04-30 [PMID: 32186328] (WB, Rat)
• A Ashok, S Chaudhary, AE Kritikos, MH Kang, D McDonald, DJ Rhee, N Singh TGFbeta2-Hepcidin Feed-Forward Loop in the Trabecular Meshwork Implicates Iron in Glaucomatous Pathology Invest. Ophthalmol. Vis. Sci., 2020-03-09;61(3):24. 2020-03-09 [PMID: 32182331]
• Wang R, Liu X, Wu J et al. Role of microRNA-122 in microcystin-leucine arginine-induced dysregulation of hepatic iron homeostasis in mice Environ. Toxicol. 2020-03-14 [PMID: 32170997]
• Sheng Y, Chen Yj, Qian Zm et Al. Cyclophosphamide induces a significant increase in iron content in the liver and spleen of mice Hum Exp Toxicol 2020-03-04 [PMID: 32129080]
• Chen X, Li D, Sun Hy et Al. Relieving ferroptosis may partially reverse neurodegeneration of the auditory cortex FEBS J. 2020-02-28 [PMID: 32112499] (WB)
• Schnetz M, Meier JK, Rehwald C et al. The Disturbed Iron Phenotype of Tumor Cells and Macrophages in Renal Cell Carcinoma Influences Tumor Growth Cancers (Basel) 2020-02-25 [PMID: 32106629] (ICC/IF, Human)
• Singh KS, Leu JI, Barnoud T et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin Nat Commun 2020-01-24 [PMID: 31980600] (WB)
• Neves JV, Gomes AC, Costa DM et al. A role for hepcidin in the anemia caused by Trypanosoma brucei infection Haematologica 2020-01-09 [PMID: 31919087] (WB, Mouse)
• Yasumura S, Naito Y, Okuno K et al. Effects of Heterozygous TfR1 (Transferrin Receptor 1) Deletion in Pathogenesis of Renal Fibrosis in Mice Hypertension 2019-12-16 [PMID: 31838906]
• Chen YJ, Qian ZM, Sheng Y et al. Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor Neurosci. Lett. 2020-01-18 [PMID: 31830506] (WB, Mouse)
• Ashok A, Chaudhary S, McDonald D, et al. Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications. Exp Eye Res 2019-12-04 [PMID: 31811823] (IF, Bovine, Human)
• Villalpando-Rodriguez G, Blankstein A, Konzelman C, Gibson S Lysosomal Destabilizing Drug Siramesine and the Dual Tyrosine Kinase Inhibitor Lapatinib Induce a Synergistic Ferroptosis through Reduced Heme Oxygenase-1 (HO-1) Levels Oxid Med Cell Longev. 2019-09-17 [PMID: 31636810]
• Nobuta H, Yang N, Ng YH et al. Oligodendrocyte Death in Pelizaeus-Merzbacher Disease Is Rescued by Iron Chelation. Cell Stem Cell 2019-10-03 [PMID: 31585094] (WB, Human)
• Thompson KJ, Wessling-Resnick M ZIP14 is degraded in response to manganese exposure Biometals 2019-09-20 [PMID: 31541377] (WB, Human)
• Okuno K, Naito Y, Yasumura S et al. Haploinsufficiency of Transferrin Receptor 1 Impairs Angiogenesis with Reduced Mitochondrial Complex I in Mice with Limb Ischemia Sci Rep 2019-09-20 [PMID: 31541184] (WB, Mouse)
• Pulos-Holmes MC, Srole DN, Juarez MG et al. Repression of ferritin light chain translation by human eIF3 Elife 2019-08-15 [PMID: 31414986] (WB, Human)
• Nagpal A, Redvers RP, Ling X et al. Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis Breast Cancer Res. 2019-08-13 [PMID: 31409375] (WB, Mouse)
• Poli M, Anower-E-Khuda F, Asperti M et al. Hepatic heparan sulfate is a master regulator of hepcidin expression and iron homeostasis in human hepatocytes and mice J. Biol. Chem. 2019-07-17 [PMID: 31315930] (WB, Human)
• Scindia Y, Wlazlo E, Leeds J et al. Protective Role of Hepcidin in Polymicrobial Sepsis and Acute Kidney Injury Front Pharmacol 2019-06-06 [PMID: 31244655] (WB, Mouse)
• Pandur E, Tamasi K, Pap R et al Fractalkine Induces Hepcidin Expression of BV-2 Microglia and Causes Iron Accumulation in SH-SY5Y Cells. Cell Mol Neurobiol. 2019-06-06 [PMID: 31172340] (WB, Human)
• Samira Lakhal-Littleton, Alexi Crosby, Matthew C. Frise, Goran Mohammad, Carolyn A. Carr, Paul A. M. Loick, Peter A. Robbins Intracellular iron deficiency in pulmonary arterial smooth muscle cells induces pulmonary arterial hypertension in mice Proceedings of the National Academy of Sciences of the United States of America 2019-06-25 [PMID: 31152133]
• Sohoni S, Ghosh P et al. Elevated Heme Synthesis and Uptake Underpin Intensified Oxidative Metabolism and Tumorigenic Functions in Non-Small Cell Lung Cancer Cells. Cancer Res 2019-05-15 [PMID: 30902795] (WB, Mouse)
• Geng S, Zhang Y, Lee C, Li L. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis Sci Adv 2019-02-01 [PMID: 30775441] (FLOW, Mouse)
• Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P, Mouse)
• Katsura Y, Ohara T, Noma K et al. A Novel Combination Cancer Therapy with Iron Chelator Targeting Cancer Stem Cells via Suppressing Stemness Cancers (Basel) 2019-02-03 [PMID: 30717462] (WB, Human)
• Ji P, Lonnerdal B, Kim K, Jinno CN. Iron Oversupplementation Causes Hippocampal Iron Overloading and Impairs Social Novelty Recognition in Nursing Piglets. J. Nutr. 2019-01-08 [PMID: 30624730] (WB, Porcine)
• Zhang MW, Zhao P, Yung WH et al. Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice Aging (Albany NY) 2018-12-16 [PMID: 30555055] (WB, Mouse)
• Miyazawa M, Bogdan AR, Tsuji Y. Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2. Cell Chem Biol. 2018-10-25 [PMID: 30449675] (WB, Human)
• Silva JM, Rodrigues S, Sampaio-Marques B et al. Dysregulation of autophagy and stress granule-related proteins in stress-driven Tau pathology. Cell Death Differ. 2018-11-15 [PMID: 30442948] (WB, Mouse)
• Schwartz AJ, Das Nk, Ramakrishnan Sk et al. Hepatic hepcidin/intestinal HIF-2a axis maintains iron absorption during iron deficiency and overload. J. Clin. Invest. 2018-10-23 [PMID: 30352047] (Western Blot, Mouse)
• Shin D, Kim EH, Lee J, Roh JL. Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer. Free Radic. Biol. Med. 2018-10-16 [PMID: 30339884] (Mouse)
• Samba-Mondonga M, Calve A, Mallette FA, Santos MM. MyD88 Regulates the Expression of SMAD4 and the Iron Regulatory Hormone Hepcidin. Front Cell Dev Biol 2018-08-31 [PMID: 30234111] (WB, Human)
• Ramakrishnan L, Pedersen SL, Toe QK et al. The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells Sci Rep 2018-08-28 [PMID: 30154413] (IF/IHC, Human)
• Sajja RK, Kaisar MA, Vijay V et al. In Vitro Modulation of Redox and Metabolism Interplay at the Brain Vascular Endothelium: Genomic and Proteomic Profiles of Sulforaphane Activity Sci Rep 2018-08-23 [PMID: 30139948] (WB, ICC/IF, Mouse)
• Ma J, Zhang FL, Zhou G et al. Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6-/- Neurons and Astrocytes Treated with Lipopolysaccharides Neurochem Res. [PMID: 29923037] (WB, Mouse)
• Kaisar MA, Sivandzade F, Bhalerao A, Cucullo L Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: In vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure Neurosci Lett. 2018-08-23 [PMID: 29879439] (WB, Mouse)
• Ashok A, Karmakar S, Chandel R et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission Exp. Eye Res. 2018-05-31 [PMID: 29859760] (ICC/IF, Human)
• Lim D, Kim KS, Jeong JH et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages Nat Commun 2018-05-29 [PMID: 29844422] (WB, Mouse)
• Blanchette-Farra N, kita D, konstorum A et al. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer. Oncogene. 2018-07-01 [PMID: 29695834] (WB, Human)
• Broide E, Reifen R, Matalon S et al. Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia. J Diabetes Res 2018-03-06 [PMID: 29693022] (WB, Human)
• Puy V, Darwiche W, Trudel S et al. Predominant role of microglia in brain iron retention in Sanfilippo syndrome, a pediatric neurodegenerative disease. Glia 2018-04-06 [PMID: 29624734] (WB, Mouse)
• McCarthy RC, SoSa JC, Gardeck AM et al. Inflammation-induced Iron Transport and Metabolism by Brain Microglia. J. Biol. Chem. 2018-04-02 [PMID: 29610275] (Mouse)
• Mertens C, Mora J, Oren B et al. Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment Oncoimmunology 2017-12-22 [PMID: 29399416] (FLOW, Mouse)
• Zhabyeyev P, Das SK, Basu R et al. TIMP3 deficiency exacerbates iron-overload mediated cardiomyopathy and liver disease Am. J. Physiol. Heart Circ. Physiol. 2018-01-26 [PMID: 29373036] (WB, Mouse)
• Raggi C, Gammella E, Correnti M et al. Dysregulation of Iron Metabolism in Cholangiocarcinoma Stem-like Cells. Sci Rep 2017-12-15 [PMID: 29247214] (WB, Human)
• Zhang FL, Hou HM, Yin ZN et al. Impairment of Hepcidin Upregulation by Lipopolysaccharide in the Interleukin-6 Knockout Mouse Brain Front Mol Neurosci 2017-11-07 [PMID: 29163042] (WB, Mouse)
• Baba Y, Higa JK, Shimada BK et al. Protective Effects of the Mechanistic Target of Rapamycin against Excess Iron and Ferroptosis in Cardiomyocytes Am. J. Physiol. Heart Circ. Physiol. 2017-11-10 [PMID: 29127238] (Mouse)
• Neves JV, Ramos MF, Moreira AC et al. Hamp1 but not Hamp2 regulates ferroportin in fish with two functionally distinct hepcidin types Sci Rep. 2017-11-01 [PMID: 29093559] (Mouse)
• Zhang W, Lv T, Huang J, Ou X. Type 4B hereditary hemochromatosis associated with a novel mutation in the SLC40A1 gene: A case report and a review of the literature Medicine (Baltimore) 2017-09-01 [PMID: 28930842] (IF/IHC, Human)
• Masaldan S, Clatworthy SAS, Gamell C et al. Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis Redox Biol 2017-09-01 [PMID: 28888202] (Western Blot, Mouse)
• Pasricha SR, Lim PJ, Duarte TL et al. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun 2017-09-01 [PMID: 28864822] (WB, Human)
• Dietrich P, Johnson IM, Alli S, Dragatsis I. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis PLoS Genet. 2017-07-01 [PMID: 28715425] (WB, Mouse)
• Basuli D, Tesfay L, Deng Z et al. Iron addiction: a novel therapeutic target in ovarian cancer Oncogene 2017-07-20 [PMID: 28319068] (WB, Human)
• Fader KA, Nault R, Kirby MP et al. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBalpha/b activation in aryl hydrocarbon receptor-elicited hepatotoxicity. Toxicol. Appl. Pharmacol. 2017-02-16 [PMID: 28213091]
• Mertens C, Akam EA, Rehwald C et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS ONE. 2016-11-02 [PMID: 27806101] (FLOW, Human)
• Zhang YT, Li FM, Guo YZ et al. (Z)-ligustilide increases ferroportin1 expression and ferritin content in ischemic SH-SY5Y cells. Eur. J. Pharmacol. 2016-10-24 [PMID: 27789221] (WB)
• Gasperini L, Meneghetti E, Legname G, Benetti F. In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. Front Neurosci. 2016-10-12 [PMID: 27729845] (WB, Mouse)
• Asperti M, Stuemler T, Poli M et al. Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation. PLoS One 2016-10-06 [PMID: 27711215] (WB)
• Kim DK, Kim YH, Jung YS et al. Orphan nuclear receptor SHP regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression. Sci Rep 2016-09-30 [PMID: 27688041] (WB)
• Marques O, Porto G, Rema A et al. Local iron homeostasis in the breast ductal carcinoma microenvironment BMC Cancer 2016-03-05 [PMID: 26944411] (IHC-P, Human)
• Theurl M, Song D, Clark E et al. Mice with hepcidin-resistant ferroportin accumulate iron in the retina. FASEB J 2016-02-01 [PMID: 26506980]
• Mori M, Ito F, Shi L et al. Ovarian endometriosis-associated stromal cells reveal persistently high affinity for iron. Redox Biol 2015-12-01 [PMID: 26498255]
• Bose C, Megyesi JK, Shah SV et al. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. PLoS One 2015-01-01 [PMID: 26305890] (WB, Mouse)
• Tesfay L, Clausen KA, Kim JW et al. Hepcidin regulation in prostate and its disruption in prostate cancer Cancer Res. 2015-04-09 [PMID: 25858146] (WB, Human)
• Gammella E, Diaz V, Recalcati S et al. Erythropoietin's inhibiting impact on hepcidin expression occurs indirectly. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2014-12-17 [PMID: 25519735] (WB, Mouse)
• Zhang L, Song Y, Cao K Et al. Hepcidin Based Nanocomposites for Enhanced Cancer Immunotherapy by Modulating Iron Export Mediated N6 Methyladenosine RNA Transcript Advanced Functional Materials 2021-10-07 (WB)
• Scheers NM, Almgren AB, Sandberg AS. Proposing a Caco-2/HepG2 cell model for in vitro iron absorption studies. The Journal of Nutritional Biochemistry 2014-03-19 [PMID: 24746839] (WB, Human)
• Gonciarz R, Jiang H, Hugelshofter C et al. In vivo Bioluminescence Imaging of Labile Iron in Xenograft Models and Liver Using FeAl-1, an Iron-Activatable Form of D-Luciferin SSRN Electronic Journal 2022-11-20
• Hao Z Understanding Heart Failure in Patients with Dilated Cardiomyopathy: A Direct Tissue Analysis from Explanted Human Hearts Thesis 2022-01-01 (WB, Human)
• Zhao Y, Guo H, Li Q et al. TREM-1 Aggravates Diabetic Associated Cognitive Impairment by Inducing Iron Deposition in Microglia via ERS Research Square 2023-04-13 (WB, Mouse)
• Tamiya H, Urushihara N, Shizuma K et al. SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-?B- and PRMT5-mediated PGC1? reduction bioRxiv 2023-04-17 (WB, Mouse)
• Sun Q, Wang Y, Hou L et al. Clozapine-N-Oxide Protects Dopaminergic Neurons Against Rotenone-Induced Neurotoxicity by Preventing Ferritinophagy-Mediated Ferroptosis Social Science Research Network 2023-09-18 (In Vivo, Mouse)
• Katz O, Reifen R, Lerner A. B-Carotene can reverse dysregulation of iron protein in an in vitro model of inflammation. Immunol. Res. 2014-11-15 [PMID: 25398638] (WB, Human)
• Jeong SM, Lee J, Finley LW et al. SIRT3 regulates cellular iron metabolism and cancer growth by repressing iron regulatory protein 1. Oncogene. 2014-06-09 [PMID: 24909164] (WB, Mouse)
  
  Details:
  WB with Ferroportin 1 antibody on whole-cell lysates of immortalized WT and SIRT3 KO MEFs showing a decreased FPN1 protein expression in SIRT3 KO compared with WT cells (Figure 3e and Supplementary Figure S3f).
• Theurl M, Nairz M, Schroll A et al. Hepcidin as a predictive factor and therapeutic target in erythropoiesis-stimulating agent treatment for anemia of chronic disease in rats. Haematologica. 2014-06-03 [PMID: 24895335] (IHC-P, WB, Rat)
  
  Details:
  Formalin fixed and paraffin-wax embedded rat (female Lewis rats) spleen and duodenum tissue sections were immunostained with 1:200 dilutionof rabbit anti-Fp1 antibdoy (NBP1-21502) in PBS/1% BSA for 1h at RT followed by donkey anti rabbit Alexa 555 for immunofluorescence detection ( Supplemental Figure S4 - Splenic Fp1 and Supplemental Figure S5 - Duodenal Fp1 immunofluorescence in ACD rats treated with ESA and/or LDN-193189). Antibody also used for WB of FP1 in spleen (Figure 3A) and liver of rats (Supplemental Figure S3).
• Vercellotti Gregory M, Khan Fatima B, Nguyen Julia et al. H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice. Front Pharmacol. 2014-04-17 [PMID: 24860503] (WB, Mouse)
• Kim DK, Jeong JH, Lee JM et al. Inverse agonist of estrogen-related receptor [gamma] controls Salmonella typhimurium infection by modulating host iron homeostasis. Nature Medicine 2014-03-23 [PMID: 24658075] (WB, Mouse)
• Labomascus BB Impact Of Maternal Pre-pregnancy Obesity On Placental Iron Transporter Protein Expression Thesis
• Scheers N, Sandberg AS. Iron Transport through Ferroportin Is Induced by Intracellular Ascorbate and Involves IRP2 and HIF2alpha. Nutrients 2014-01-03 [PMID: 24394537] (WB, Human)
• Diaz V, Gammella E, Recalcati S et al. Liver iron modulates hepcidin expression during chronically elevated erythropoiesis in mice. Hepatology 2013-06-06 [PMID: 23744538] (WB, Mouse)
• Choi JS, Koh IU, Lee HJ et al. Effects of excess dietary iron fat on glucose lipid metabolism. J Nutr Biochem 2013-05-02 [PMID: 23643521] (WB, Mouse)
• Bayeva M, Khechaduri A, Puig S et al. mTOR Regulates Cellular Iron Homeostasis through Tristetraprolin Cell Metab 2012-10-23 [PMID: 23102618] (WB, Mouse)
• Li X, Jiang X, Chu H et al. Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition Chinese Herbal Medicines 2020-12-01 (WB, Rat)
• Vanoaica L et al. Intestinal ferritin h is required for an accurate control of iron absorption. Cell Metab 12(3):273-82. 2010-01-01 [PMID: 20816093] (WB, Mouse)
• Hazlett HF Characterizing the Role of Human Macrophage Iron Metabolism in Inflammatory Lung Disease Thesis 2021-01-01
• Hao Z Understanding Heart Failure in Patients with Dilated Cardiomyopathy: A Direct Tissue Analysis from Explanted Human Hearts Thesis 2022 Jan 01 (WB, Human)

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (8)

Average Rating: 4.6

(Based on 8 reviews)

We have 8 reviews tested in 4 species: Human, Mouse, Bovine, Pig.

reviewed by: Verified Customer IHC-P Mouse 05/09/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse small intestine,small intestines
• Species: Mouse
• Lot: D127012-2

Comments

• Comments: Tissue fixed in formalin overnight; embedded in paraffin; ~5micro sections on slides; dehydrated; antigen retrieval using citrate buffer in rice steamer for 20mins; 1 hour blocking without permeabilization; overnight primary ab incubation @ 1:200 (NBP1-21502) at 4c; 2-hr secondary ab incubation at room temp; mounting media with DAPI.

reviewed by: Verified Customer WB Mouse 08/08/2018

Summary

• Application: Western Blot
• Sample Tested: Adult pancreas
• Species: Mouse
• Lot: F-1

reviewed by: Verified Customer WB Pig 07/12/2018

Summary

• Application: Western Blot
• Sample Tested: duodenal mucosa
• Species: Pig
• Lot: F-1

reviewed by: Verified Customer IHC-P Human 04/04/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult eye and muscle tissue (labeled E and M, respectively)
• Species: Human
• Lot: E-2

Comments

• Comments: 1:100

reviewed by: Verified Customer WB Bovine 04/04/2018

Summary

• Application: Western Blot
• Sample Tested: Adult eye
• Species: Bovine
• Lot: E-2

Comments

• Comments: 1:1000

reviewed by: Verified Customer IHC-P Bovine 10/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: eye
• Species: Bovine
• Lot: E-2

Comments

• Comments: use at 1:100

reviewed by: Verified Customer IHC Mouse 06/27/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Ajay Ashok WB Human 06/13/2017

Summary

• Application: Western Blot
• Sample Tested: Human microglia cell lysate
• Species: Human

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 5 of 5 FAQs).

1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
   • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
2. Would it be possible to conjugate this antibody to PE or PerCP?
   • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
   • The immunogen used for this Ferroportin/SLC40A1 Antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.
4. I can’t get positive a result in IHC-P using NBP1-21502 (tissue: atherosclerosis hemorrhage plaque). PV-6001 and DAB is working. Can you make optimization suggestions on how to get this antibody to work?
   • Optimization could really make a difference as we have seen some staining with the same antibody on frozen section. The main thing would be to focus on antigen retrieval step and the buffer used. For the antigen retrieval method, we would suggest an increase to at least 10-20 minutes to the pressure cooker step of the HIER method; the duration of the retrieval step can greatly determine if the antigen will be exposed or not for the antigen binding site of the antibody to recognize the protein. Also, I the incubation time for peroxidase blocking time could be reduced from 30min to 15 min, as we have mentioned. Since this is a TMP, permeabilization reagents should be used for the experiment. You can review what we have available on our site in terms of our general protocols:HIER and IHC-P​
5. We are having difficulty producing a clean blot, i.e. when we probe our western blots with the recommended concentration of 1.0 ug/ml, we are seeing multiple bands. The order of the molecular weight ladder we used is as followed: 250, 130, 100, 70, 55, 35, 25, 15, and 10 kDa. Are you able to indicate what band may be ferroportin.?
   • There is a western blot protocol specific to this antibody that can be found on the datasheet; it differs from your protocol quite a lot. I would give this protocol a try and see if you get better results.Although I am uncertain of your blocking method, we find that BSA and PVDF can give high non-specific background so the nitrocellulose and NFDM are recommend for this antibody. In addition, there seems to be no protein expression of the SLC40A1 in liver or spleen based on Human Protein Atlas.https://www.proteinatlas.org/ENSG00000138449-SLC40A1/tissueA good positive control might be bone marrow.

Bioinformatics

• Gene Symbol: SLC40A1
• Entrez:
  • 30061(Human)
  • 53945(Mouse)
• Uniprot:
  • Q9NP59()