Ferroportin/SLC40A1 Antibody

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Western blot of human intestine lysate (Molecular weight: 62.5 KDa) using Ferroportin/SLC40A1 Antibody.

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed using Ferroportin/SLC40A1 Antibody(NBP1-21502; 1:1000 dilution) and GAPDH (loading control). Image from verified customer review.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Mouse Ferroportin/SLC40A1, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. Proteins of subcellular fractions isolated from livers of wt-, ms-, and LRS-treated mice (n = 4) were run on a western blot (30 ug protein/lane) and immunostained for microsomal ferroportin. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2014.00079/abstract), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with Ferroportin/SLC40A1 Antibody at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine small intestinal section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The antibody primarily developed a membranous staining pattern in the intestinal epithelial cells.

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Western blot of bovine adult eye using Ferroportin/SLC40A1 Antibody. Image from verified customer review.

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 clones overexpressing heparanase showed a reduction of hepcidin expression and indices of iron loading. Two stable clones of HepG2 cells transfected with pcDNA3.1-HPA (HPA3 and HPA6) were analyzed for hepcidin expression, BMP/SMAD signaling and indices of iron status. Western blot of Ferroportin/SLC40A1 and its densitometry. The values are expressed as-dCt (for HPA mRNA) or as fold change over the control (MOCK) (for hepcidin and Id1 mRNA). The images are representative from three different analyses. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371:journal.pone.0164183), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine liver section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The representative image shows intense staining in the cellular membranes, whereas, a relatively milder postivity was observed in the cytoplasm of hepatocytes.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of Ferroportin/SLC40A1 Antibody. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin/SLC40A1 antibody. Image from verified customer review.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with NBP1-21502AF647 (blue) and a matched isotype control (orange, NBP2-24893AF647). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ferroportin/SLC40A1 Antibody Summary

• Immunogen: This Ferroportin/SLC40A1 Antibody was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]
• Localization: Cell membrane (high), Cytoplasm (low)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC40A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
• Application Notes: In Western blot, a band is seen at ~62 kDa.
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Ferroportin/SLC40A1 Protein (NBP1-21502PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Antibody tested in Flow Cytometry by verified customer through our Innovator's Reward Program.

Alternate Names for Ferroportin/SLC40A1 Antibody

• Ferroportin
• Ferroportin-1
• FPN1
• FPN1IREG1ferroportin 1
• HFE4
• HFE4ferroportin-1
• IREG1
• iron regulated gene 1
• Iron-regulated transporter 1
• member 3
• MST079
• MSTP079
• MTP1
• putative ferroportin 1 variant IIIB
• SLC11A3
• SLC11A3iron regulated gene 1
• SLC40A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)
• solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
• solute carrier family 40 (iron-regulated transporter), member 1
• solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(89)

Publications using NBP1-21502

• FernAndez-MendIvil C, Luengo E, Trigo-Alonso P et al. Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism Redox Biol Nov 6 2020 [PMID: 33212416] (WB)
• Verma N, Vinik Y, Saroha A et al. Synthetic lethal combination targeting BET uncovered intrinsic susceptibility of TNBC to ferroptosis Sci. Adv. Aug 1 2020 [PMID: 32937365]
• Yang G, Qian C, Zhang C et al. Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats Transl Res Sep 12 2020 [PMID: 32932001] (WB, Rat)
• Renassia C, Louis S, Cuvellier S et al. Neutrophils from hereditary hemochromatosis patients are protected from iron excess and are primed Blood Adv Aug 25 2020 [PMID: 32810223] (WB, Human)
• Lin PL, Tang HH, Wu SY et al. Saponin Formosanin C-induced Ferritinophagy and Ferroptosis in Human Hepatocellular Carcinoma Cells Antioxidants (Basel) Jul 29 2020 [PMID: 32751249] (WB, Human)
• Kim HY, Kwon WY, Park JB et al. Hepatic STAMP2 mediates recombinant FGF21-induced improvement of hepatic iron overload in nonalcoholic fatty liver disease FASEB J. Jul 28 2020 [PMID: 32721044]
• Pease NA, Shephard MS, Sertorio M et al. DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages Cancers (Basel) Jul 17 2020 [PMID: 32708944] (WB, Mouse)
• Tsuji Y Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SLC40A1 (ferroportin) PLoS ONE Jul 9 2020 [PMID: 32645092] (WB, KD, Human)
• Sheng Y, Chen Yj, Qian Zm et Al. Cyclophosphamide induces a significant increase in iron content in the liver and spleen of mice Hum Exp Toxicol Mar 4 2020 [PMID: 32129080]
• Chen X, Li D, Sun Hy et Al. Relieving ferroptosis may partially reverse neurodegeneration of the auditory cortex FEBS J. Feb 28 2020 [PMID: 32112499] (WB)
• Kammerer L, Mohammad G, Wolna M, Robbins P Fetal liver hepcidin supports acquisition of iron stores in utero BioRxiv 2019 Oct 09 (IHC-Fr, Mouse)
• Yamaguchi H, Honda S, Torii S et al. Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration Nat Commun Oct 20 2020 [PMID: 33082312] (WB, ICC/IF, Human)
• Lee J, You JH, Shin D, Roh JL Inhibition of Glutaredoxin 5 predisposes Cisplatin-resistant Head and Neck Cancer Cells to Ferroptosis Theranostics Jun 19 2020 [PMID: 32685019] (WB, Human)
• Hazlett HF, Hampton TH, Aridgides DS et al. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival Sci Rep Jul 2 2020 [PMID: 32616918]
• Li S, Qian ZM, Xu G et al. Hepatocyte growth factor protects PC12 cells against OGD/R-induced injury by reducing iron Biosci. Rep. Apr 30 2020 [PMID: 32186328] (WB, Rat)
• Wang R, Liu X, Wu J et al. Role of microRNA-122 in microcystin-leucine arginine-induced dysregulation of hepatic iron homeostasis in mice Environ. Toxicol. Mar 14 2020 [PMID: 32170997]
• Schnetz M, Meier JK, Rehwald C et al. The Disturbed Iron Phenotype of Tumor Cells and Macrophages in Renal Cell Carcinoma Influences Tumor Growth Cancers (Basel) Feb 25 2020 [PMID: 32106629] (ICC/IF, Human)
• Singh KS, Leu JI, Barnoud T et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin Nat Commun Jan 24 2020 [PMID: 31980600] (WB)
• Neves JV, Gomes AC, Costa DM et al. A role for hepcidin in the anemia caused by Trypanosoma brucei infection Haematologica Jan 9 2020 [PMID: 31919087] (WB, Mouse)
• Yasumura S, Naito Y, Okuno K et al. Effects of Heterozygous TfR1 (Transferrin Receptor 1) Deletion in Pathogenesis of Renal Fibrosis in Mice Hypertension Dec 16 2019 [PMID: 31838906]
• Chen YJ, Qian ZM, Sheng Y et al. Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor Neurosci. Lett. Jan 18 2020 [PMID: 31830506] (WB, Mouse)
• Ashok A, Chaudhary S, McDonald D, et al. Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications. Exp Eye Res 2019 Dec 4 [PMID: 31811823] (IF, Bovine, Human)
• Villalpando-Rodriguez G, Blankstein A, Konzelman C, Gibson S Lysosomal Destabilizing Drug Siramesine and the Dual Tyrosine Kinase Inhibitor Lapatinib Induce a Synergistic Ferroptosis through Reduced Heme Oxygenase-1 (HO-1) Levels Oxid Med Cell Longev. Sep 17 2019 [PMID: 31636810]
• Nobuta H, Yang N, Ng YH et al. Oligodendrocyte Death in Pelizaeus-Merzbacher Disease Is Rescued by Iron Chelation. Cell Stem Cell 2019 Oct 03 [PMID: 31585094] (WB, Human)
• Thompson KJ, Wessling-Resnick M ZIP14 is degraded in response to manganese exposure Biometals Sep 20 2019 [PMID: 31541377] (WB, Human)
• Okuno K, Naito Y, Yasumura S et al. Haploinsufficiency of Transferrin Receptor 1 Impairs Angiogenesis with Reduced Mitochondrial Complex I in Mice with Limb Ischemia Sci Rep Sep 20 2019 [PMID: 31541184] (WB, Mouse)
• Pulos-Holmes MC, Srole DN, Juarez MG et al. Repression of ferritin light chain translation by human eIF3 Elife Aug 15 2019 [PMID: 31414986] (WB, Human)
• Nagpal A, Redvers RP, Ling X et al. Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis Breast Cancer Res. Aug 13 2019 [PMID: 31409375] (WB, Mouse)
• Poli M, Anower-E-Khuda F, Asperti M et al. Hepatic heparan sulfate is a master regulator of hepcidin expression and iron homeostasis in human hepatocytes and mice J. Biol. Chem. Jul 17 2019 [PMID: 31315930] (WB, Human)
• Scindia Y, Wlazlo E, Leeds J et al. Protective Role of Hepcidin in Polymicrobial Sepsis and Acute Kidney Injury Front Pharmacol Jun 6 2019 [PMID: 31244655] (WB, Mouse)
• Pandur E, Tamasi K, Pap R et al Fractalkine Induces Hepcidin Expression of BV-2 Microglia and Causes Iron Accumulation in SH-SY5Y Cells. Cell Mol Neurobiol. 2019 Jun 06 [PMID: 31172340] (WB, Human)
• Sohoni S, Ghosh P et al. Elevated Heme Synthesis and Uptake Underpin Intensified Oxidative Metabolism and Tumorigenic Functions in Non-Small Cell Lung Cancer Cells. Cancer Res May 15 2019 [PMID: 30902795] (WB, Mouse)
• Geng S, Zhang Y, Lee C, Li L. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis Sci Adv Feb 1 2019 [PMID: 30775441] (Flow, Mouse)
• Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb 5 2019 [PMID: 30723259] (IHC-P, Mouse)
• Katsura Y, Ohara T, Noma K et al. A Novel Combination Cancer Therapy with Iron Chelator Targeting Cancer Stem Cells via Suppressing Stemness Cancers (Basel) Feb 3 2019 [PMID: 30717462] (WB, Human)
• Zhang MW, Zhao P, Yung WH et al. Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice Aging (Albany NY) Dec 16 2018 [PMID: 30555055] (WB, Mouse)
• Miyazawa M, Bogdan AR, Tsuji Y. Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2. Cell Chem Biol. Oct 25 2018 [PMID: 30449675] (WB, Human)
• Silva JM, Rodrigues S, Sampaio-Marques B et al. Dysregulation of autophagy and stress granule-related proteins in stress-driven Tau pathology. Cell Death Differ. Nov 15 2018 [PMID: 30442948] (WB, Mouse)
• Shin D, Kim EH, Lee J, Roh JL. Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer. Free Radic. Biol. Med. Oct 16 2018 [PMID: 30339884] (Mouse)
• Samba-Mondonga M, Calve A, Mallette FA, Santos MM. MyD88 Regulates the Expression of SMAD4 and the Iron Regulatory Hormone Hepcidin. Front Cell Dev Biol Aug 31 2018 [PMID: 30234111] (WB, Human)
• Jiang H, Muir R, Gonciarz R et al. Exploiting KRAS-Driven Ferroaddiction in Cancer Through Ferrous Iron-Activatable Drug Conjugates (FeADC) SSRN Journal May 8 2020
• Ji P, Lonnerdal B, Kim K, Jinno CN. Iron Oversupplementation Causes Hippocampal Iron Overloading and Impairs Social Novelty Recognition in Nursing Piglets. J. Nutr. Jan 8 2019 [PMID: 30624730] (WB, Porcine)
• Ramakrishnan L, Pedersen SL, Toe QK et al. The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells Sci Rep Aug 28 2018 [PMID: 30154413] (IHC, Human)
• Li X, Jiang X, Chu H et al. Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition Chinese Herbal Medicines Dec 1 2020 (WB, Rat)
• Kaisar MA, Sivandzade F, Bhalerao A, Cucullo L Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: In vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure Neurosci Lett. 2018 Aug 23 [PMID: 29879439] (WB, Mouse)
• Lim D, Kim KS, Jeong JH et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages Nat Commun May 29 2018 [PMID: 29844422] (WB, Mouse)
• Sajja RK, Kaisar MA, Vijay V et al. In Vitro Modulation of Redox and Metabolism Interplay at the Brain Vascular Endothelium: Genomic and Proteomic Profiles of Sulforaphane Activity Sci Rep Aug 23 2018 [PMID: 30139948] (ICC/IF, WB, Mouse)
• Ma J, Zhang FL, Zhou G et al. Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6-/- Neurons and Astrocytes Treated with Lipopolysaccharides Neurochem Res. [PMID: 29923037] (WB, Mouse)
• Ashok A, Karmakar S, Chandel R et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission Exp. Eye Res. May 31 2018 [PMID: 29859760] (ICC/IF, Human)
• Blanchette-Farra N, kita D, konstorum A et al. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer. Oncogene. Jul 1 2018 [PMID: 29695834] (WB, Human)
• Broide E, Reifen R, Matalon S et al. Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia. J Diabetes Res Mar 6 2018 [PMID: 29693022] (WB, Human)
• Labomascus BB Impact Of Maternal Pre-pregnancy Obesity On Placental Iron Transporter Protein Expression Thesis
• Puy V, Darwiche W, Trudel S et al. Predominant role of microglia in brain iron retention in Sanfilippo syndrome, a pediatric neurodegenerative disease. Glia Apr 6 2018 [PMID: 29624734] (WB, Mouse)
• McCarthy RC, SoSa JC, Gardeck AM et al. Inflammation-induced Iron Transport and Metabolism by Brain Microglia. J. Biol. Chem. Apr 2 2018 [PMID: 29610275] (Mouse)
• Mertens C, Mora J, Oren B et al. Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment Journal OncoImmunology 2017 Nov 27 [PMID: 29399416] (Flow, Mouse)
• Zhabyeyev P, Das SK, Basu R et al. TIMP3 deficiency exacerbates iron-overload mediated cardiomyopathy and liver disease Am. J. Physiol. Heart Circ. Physiol. 2018 Jan 26 [PMID: 29373036] (WB, Mouse)
• Raggi C, Gammella E, Correnti M et al. Dysregulation of Iron Metabolism in Cholangiocarcinoma Stem-like Cells. Sci Rep 2017 Dec 15 [PMID: 29247214] (WB, Human)
• Zhang FL, Hou HM, Yin ZN et al. Impairment of Hepcidin Upregulation by Lipopolysaccharide in the Interleukin-6 Knockout Mouse Brain Front Mol Neurosci 2017 Nov 07 [PMID: 29163042] (WB, Mouse)
• Baba Y, Higa JK, Shimada BK et al. Protective Effects of the Mechanistic Target of Rapamycin against Excess Iron and Ferroptosis in Cardiomyocytes Am. J. Physiol. Heart Circ. Physiol. 2017 Nov 10 [PMID: 29127238] (Mouse)
• Neves JV, Ramos MF, Moreira AC et al. Hamp1 but not Hamp2 regulates ferroportin in fish with two functionally distinct hepcidin types Sci Rep. 2017 Nov 01 [PMID: 29093559] (Mouse)
• Zhang W, Lv T, Huang J, Ou X. Type 4B hereditary hemochromatosis associated with a novel mutation in the SLC40A1 gene: A case report and a review of the literature Medicine (Baltimore) 2017 Sep 01 [PMID: 28930842] (IHC, Human)
• Pasricha SR, Lim PJ, Duarte TL et al. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun 2017 Sep 01 [PMID: 28864822] (WB, Human)
• Dietrich P, Johnson IM, Alli S, Dragatsis I. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis PLoS Genet. 2017 Jul 01 [PMID: 28715425] (WB, Mouse)
• Basuli D, Tesfay L, Deng Z et al. Iron addiction: a novel therapeutic target in ovarian cancer Oncogene 2017 Jul 20 [PMID: 28319068] (WB, Human)
• Fader KA, Nault R, Kirby MP et al. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBalpha/b activation in aryl hydrocarbon receptor-elicited hepatotoxicity. Toxicol. Appl. Pharmacol. 2017 Feb 16 [PMID: 28213091]
• Mertens C, Akam EA, Rehwald C et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS ONE. Nov 2 2016 [PMID: 27806101] (FLOW, Human)
• Zhang YT, Li FM, Guo YZ et al. (Z)-ligustilide increases ferroportin1 expression and ferritin content in ischemic SH-SY5Y cells. Eur. J. Pharmacol. Oct 24 2016 [PMID: 27789221] (WB)
• Gasperini L, Meneghetti E, Legname G, Benetti F. In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. Front Neurosci. Oct 12 2016 [PMID: 27729845] (WB, Mouse)
• Asperti M, Stuemler T, Poli M et al. Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation. PLoS One 2016 Oct 06 [PMID: 27711215] (WB)
• Kim DK, Kim YH, Jung YS et al. Orphan nuclear receptor SHP regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression. Sci Rep 2016 Sep 30 [PMID: 27688041] (WB)
• Marques O, Porto G, Rema A et al. Local iron homeostasis in the breast ductal carcinoma microenvironment BMC Cancer Mar 5 2016 [PMID: 26944411] (IHC-P, Human)
• Theurl M, Song D, Clark E et al. Mice with hepcidin-resistant ferroportin accumulate iron in the retina. FASEB J 2016 Feb [PMID: 26506980]
• Mori M, Ito F, Shi L et al. Ovarian endometriosis-associated stromal cells reveal persistently high affinity for iron. Redox Biol 2015 Dec [PMID: 26498255]
• Bose C, Megyesi JK, Shah SV et al. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. PLoS One 2015 [PMID: 26305890] (WB, Mouse)
• Tesfay L, Clausen KA, Kim JW et al. Hepcidin regulation in prostate and its disruption in prostate cancer Cancer Res. 2015 Apr 09 [PMID: 25858146] (WB, Human)
• Gammella E, Diaz V, Recalcati S et al. Erythropoietin's inhibiting impact on hepcidin expression occurs indirectly. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2014 Dec 17 [PMID: 25519735] (WB, Mouse)
• Katz O, Reifen R, Lerner A. B-Carotene can reverse dysregulation of iron protein in an in vitro model of inflammation. Immunol. Res. 2014 Nov 15 [PMID: 25398638] (WB, Human)
• Jeong SM, Lee J, Finley LW et al. SIRT3 regulates cellular iron metabolism and cancer growth by repressing iron regulatory protein 1. Oncogene. 2014 Jun 09 [PMID: 24909164] (WB, Mouse)
  
  Details:
  WB with Ferroportin 1 antibody on whole-cell lysates of immortalized WT and SIRT3 KO MEFs showing a decreased FPN1 protein expression in SIRT3 KO compared with WT cells (Figure 3e and Supplementary Figure S3f).
• Theurl M, Nairz M, Schroll A et al. Hepcidin as a predictive factor and therapeutic target in erythropoiesis-stimulating agent treatment for anemia of chronic disease in rats. Haematologica. 2014 Jun 03 [PMID: 24895335] (WB, IHC-P, Rat)
  
  Details:
  Formalin fixed and paraffin-wax embedded rat (female Lewis rats) spleen and duodenum tissue sections were immunostained with 1:200 dilutionof rabbit anti-Fp1 antibdoy (NBP1-21502) in PBS/1% BSA for 1h at RT followed by donkey anti rabbit Alexa 555 for immunofluorescence detection ( Supplemental Figure S4 - Splenic Fp1 and Supplemental Figure S5 - Duodenal Fp1 immunofluorescence in ACD rats treated with ESA and/or LDN-193189). Antibody also used for WB of FP1 in spleen (Figure 3A) and liver of rats (Supplemental Figure S3).
• Vercellotti Gregory M, Khan Fatima B, Nguyen Julia et al. H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice. Front Pharmacol. 2014 Apr 17 [PMID: 24860503] (WB, Mouse)
• Scheers NM, Almgren AB, Sandberg AS. Proposing a Caco-2/HepG2 cell model for in vitro iron absorption studies. The Journal of Nutritional Biochemistry 3/19/2014 [PMID: 24746839] (WB, Human)
• Kim DK, Jeong JH, Lee JM et al. Inverse agonist of estrogen-related receptor [gamma] controls Salmonella typhimurium infection by modulating host iron homeostasis. Nature Medicine 3/23/2014 [PMID: 24658075] (WB, Mouse)
• Scheers N, Sandberg AS. Iron Transport through Ferroportin Is Induced by Intracellular Ascorbate and Involves IRP2 and HIF2alpha. Nutrients 2014 Jan 3 [PMID: 24394537] (WB, Human)
• Diaz V, Gammella E, Recalcati S et al. Liver iron modulates hepcidin expression during chronically elevated erythropoiesis in mice. Hepatology 2013 Jun 6 [PMID: 23744538] (WB, Mouse)
• Choi JS, Koh IU, Lee HJ et al. Effects of excess dietary iron fat on glucose lipid metabolism. J Nutr Biochem 2013 May 2 [PMID: 23643521] (WB, Mouse)
• Bayeva M, Khechaduri A, Puig S et al. mTOR Regulates Cellular Iron Homeostasis through Tristetraprolin Cell Metab 2012 Oct 23 [PMID: 23102618] (WB, Mouse)
• Vanoaica L et al. Intestinal ferritin h is required for an accurate control of iron absorption. Cell Metab 12(3):273-82. 2010 [PMID: 20816093] (WB, Mouse)

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (7)

Average Rating: 4.7

(Based on 7 reviews)

We have 7 reviews tested in 5 species: Human, Mouse, Bovine, Pig, bovine.

reviewed by: Anonymous WB Mouse 08/08/2018

Summary

• Application: Western Blot
• Sample Tested: Adult pancreas
• Species: Mouse
• Lot: F-1

reviewed by: Anonymous WB Pig 07/12/2018

Summary

• Application: Western Blot
• Sample Tested: duodenal mucosa
• Species: Pig
• Lot: F-1

reviewed by: Anonymous IHC-P Human 04/04/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult eye and muscle tissue (labeled E and M, respectively)
• Species: Human
• Lot: E-2

Comments

• Comments: 1:100

reviewed by: Anonymous WB bovine 04/04/2018

Summary

• Application: Western Blot
• Sample Tested: Adult eye
• Species: bovine
• Lot: E-2

Comments

• Comments: 1:1000

reviewed by: Anonymous IHC-P Bovine 10/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: eye
• Species: Bovine
• Lot: E-2

Comments

• Comments: use at 1:100

reviewed by: Anonymous IHC Mouse 06/27/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Ajay Ashok WB Human 06/13/2017

Summary

• Application: Western Blot
• Sample Tested: Human microglia cell lysate
• Species: Human

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 5 of 5 FAQs).

1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
   • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
2. Would it be possible to conjugate this antibody to PE or PerCP?
   • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
   • The immunogen used for this Ferroportin/SLC40A1 Antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.
4. I can’t get positive a result in IHC-P using NBP1-21502 (tissue: atherosclerosis hemorrhage plaque). PV-6001 and DAB is working. Can you make optimization suggestions on how to get this antibody to work?
   • Optimization could really make a difference as we have seen some staining with the same antibody on frozen section. The main thing would be to focus on antigen retrieval step and the buffer used. For the antigen retrieval method, we would suggest an increase to at least 10-20 minutes to the pressure cooker step of the HIER method; the duration of the retrieval step can greatly determine if the antigen will be exposed or not for the antigen binding site of the antibody to recognize the protein. Also, I the incubation time for peroxidase blocking time could be reduced from 30min to 15 min, as we have mentioned. Since this is a TMP, permeabilization reagents should be used for the experiment. You can review what we have available on our site in terms of our general protocols:HIER: https://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.htmlIHC-P: https://www.novusbio.com/support/support-by-application/immunohistochemistry-paraffin/protocol.html
5. We are having difficulty producing a clean blot, i.e. when we probe our western blots with the recommended concentration of 1.0 ug/ml, we are seeing multiple bands. The order of the molecular weight ladder we used is as followed: 250, 130, 100, 70, 55, 35, 25, 15, and 10 kDa. Are you able to indicate what band may be ferroportin.?
   • There is a western blot protocol specific to this antibody that can be found on the datasheet; it differs from your protocol quite a lot. I would give this protocol a try and see if you get better results.https://resources.novusbio.com/protocols/Western-Blot-Protocol-for-Ferroportin-1-Antibody-(NBP1-21502).pdfAlthough I am uncertain of your blocking method, we find that BSA and PVDF can give high non-specific background so the nitrocellulose and NFDM are recommend for this antibody. In addition, there seems to be no protein expression of the SLC40A1 in liver or spleen based on Human Protein Atlas.https://www.proteinatlas.org/ENSG00000138449-SLC40A1/tissueA good positive control might be bone marrow.

Bioinformatics

• Gene Symbol: SLC40A1
• Entrez:
  • 30061(Human)
  • 53945(Mouse)
• Uniprot:
  • Q9NP59()