Ferroportin/SLC40A1 Antibody

Images

 
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Human intestine lysate.
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Mouse ferroportin, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed for FPN1 (NBP1-21502; 1:1000 dilution) and GAPDH (loading ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with NBP1-21502 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with ...read more
Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin 1 at 5.0 ug/ml ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of FPN1 / SLC40A1 protein in murine small intestinal section using Ferroportin 1 antibody at a dilution of 1:200. The antibody ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Bovine adult eye. Image from verified customer review.
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of FPN1 / SLC40A1 protein in murine liver section using Ferroportin 1 antibody at a dilution of 1:200. The representative image shows ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of NBP1-21502. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF647 (blue) and a matched isotype control (orange, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ferroportin/SLC40A1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]
Localization
Cell membrane (high), Cytoplasm (low)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC40A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
Application Notes
In Western blot, a band is seen at ~62 kDa.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Reviewed Applications
Read 7 Reviews rated 4.7
using
NBP1-21502 in the following applications:

Publications
Read Publications using
NBP1-21502 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Antibody tested in Flow Cytometry by verified customer through our Innovator's Reward Program.

Alternate Names for Ferroportin/SLC40A1 Antibody

  • Ferroportin
  • Ferroportin-1
  • FPN1
  • FPN1IREG1ferroportin 1
  • HFE4
  • HFE4ferroportin-1
  • IREG1
  • Iron-regulated transporter 1
  • member 3
  • MST079
  • MTP1
  • putative ferroportin 1 variant IIIB
  • SLC11A3
  • SLC11A3iron regulated gene 1
  • SLC40A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)
  • solute carrier family 40 (iron-regulated transporter), member 1
  • solute carrier family 40 member 1

Background

Ferroportin 1 (FPN1 or SLC40A1) is an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen etc.) that play key role in intestinal iron absorption as well as cellular iron release, and mediates iron efflux in the presence of ferroxidases hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in the iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. FPN1 can be regulated transcriptionally, post-transcriptionally, through mRNA stability and post-translationally through protein turnover and its regulation is critical for iron homeostasis because alterations in the same may lead to either iron deficiency or iron overload. FPN1 transports iron in ferrous form whereas plasma transferrin only binds iron's ferric form, and ferroxidases are key players in oxidizing iron transported by FPN1, and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types in the body utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1. Defects in FPN1 have been linked to hemochromatosis type 4 (HFE4), an autosomal dominant iron-loading disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-59337
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00004891-M01
Species: Hu, Bv
Applications: WB, ELISA, IHC, IHC-P
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-84071
Species: Hu
Applications: WB, IHC, IHC-P
AF7509
Species: Hu, Mu, Rt
Applications: WB, IHC
AF3634
Species: Mu
Applications: WB
MAB3120
Species: Hu
Applications: WB, Simple Western
NBP1-85483
Species: Hu
Applications: IHC, IHC-P
NB300-914
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA
NBP2-12553
Species: Hu
7270-IL/CF
Species: Hu
MAB8400
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB200-585
Species: Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP2-80524
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-21502
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P

Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(66)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Porcine.

We have publications tested in 8 applications: FLOW, Flow, ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(2)
ICC/IF
(2)
IF
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(3)
WB
(46)
All Applications
Filter By Species
Human
(23)
Mouse
(32)
Rat
(1)
Bovine
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using NBP1-21502 Applications Species
Yasumura S, Naito Y, Okuno K et al. Effects of Heterozygous TfR1 (Transferrin Receptor 1) Deletion in Pathogenesis of Renal Fibrosis in Mice Hypertension Dec 16 2019 [PMID: 31838906]
Chen YJ, Qian ZM, Sheng Y et al. Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor Neurosci. Lett. Jan 18 2020 [PMID: 31830506] (WB, Mouse) WB Mouse
Ashok A, Chaudhary S, McDonald D, et al. Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications. Exp Eye Res 2019 Dec 4 [PMID: 31811823] (IF, Bovine, Human) IF Bovine, Human
Villalpando-Rodriguez G, Blankstein A, Konzelman C, Gibson S Lysosomal Destabilizing Drug Siramesine and the Dual Tyrosine Kinase Inhibitor Lapatinib Induce a Synergistic Ferroptosis through Reduced Heme Oxygenase-1 (HO-1) Levels Oxid Med Cell Longev. Sep 17 2019 [PMID: 31636810]
Labomascus BB Impact Of Maternal Pre-pregnancy Obesity On Placental Iron Transporter Protein Expression Thesis
Nobuta H, Yang N, Ng YH et al. Oligodendrocyte Death in Pelizaeus-Merzbacher Disease Is Rescued by Iron Chelation. Cell Stem Cell 2019 Oct 03 [PMID: 31585094] (WB, Human) WB Human
Thompson KJ, Wessling-Resnick M ZIP14 is degraded in response to manganese exposure Biometals Sep 20 2019 [PMID: 31541377] (WB, Human) WB Human
Okuno K, Naito Y, Yasumura S et al. Haploinsufficiency of Transferrin Receptor 1 Impairs Angiogenesis with Reduced Mitochondrial Complex I in Mice with Limb Ischemia Sci Rep Sep 20 2019 [PMID: 31541184] (WB, Mouse) WB Mouse
Pulos-Holmes MC, Srole DN, Juarez MG et al. Repression of ferritin light chain translation by human eIF3 Elife Aug 15 2019 [PMID: 31414986] (WB, Human) WB Human
Nagpal A, Redvers RP, Ling X et al. Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis Breast Cancer Res. Aug 13 2019 [PMID: 31409375] (WB, Mouse) WB Mouse
Show All 66 Publications.

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (7) 4.77

Average Rating: 4.7
(Based on 7 reviews)
We have 7 reviews tested in 5 species: Human, Mouse, Bovine, Pig, bovine.

Reviews using NBP1-21502:
Filter by Applications
WB
(4)
IHC-P
(2)
IHC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Bovine
(1)
Pig
(1)
bovine
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
WB Mouse 08/08/2018
View

Summary

ApplicationWestern Blot
Sample TestedAdult pancreas
SpeciesMouse
LotF-1
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
WB Pig 07/12/2018
View

Summary

ApplicationWestern Blot
Sample Testedduodenal mucosa
SpeciesPig
LotF-1
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
IHC-P Human 04/04/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult eye and muscle tissue (labeled E and M, respectively)
SpeciesHuman
LotE-2

Comments

Comments1:100
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
WB bovine 04/04/2018
View

Summary

ApplicationWestern Blot
Sample TestedAdult eye
Speciesbovine
LotE-2

Comments

Comments1:1000
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
IHC-P Bovine 10/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedeye
SpeciesBovine
LotE-2

Comments

Commentsuse at 1:100
Immunohistochemistry Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
IHC Mouse 06/27/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Ajay Ashok
WB Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 3 of 3 FAQs).

  1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
    • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
  2. Would it be possible to conjugate this antibody to PE or PerCP?
    • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
  3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
    • The immunogen used for our Ferroportin antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-21502AF350
Alexa Fluor 405 NBP1-21502AF405
Alexa Fluor 488 NBP1-21502AF488
Alexa Fluor 532 NBP1-21502AF532
Alexa Fluor 594 NBP1-21502AF594
Alexa Fluor 647 NBP1-21502AF647
Alexa Fluor 700 NBP1-21502AF700
Alexa Fluor 750 NBP1-21502AF750
Allophycocyanin/Cy7 NBP1-21502APCCY7
Biotin NBP1-21502B
DyLight 350 NBP1-21502UV
DyLight 405 NBP1-21502V
DyLight 488 NBP1-21502G
DyLight 550 NBP1-21502R
DyLight 594 NBP1-21502DL594
DyLight 650 NBP1-21502C
DyLight 680 NBP1-21502FR
DyLight 755 NBP1-21502IR
FITC NBP1-21502F
HRP NBP1-21502H
Janelia Fluor 549 NBP1-21502JF549
Janelia Fluor 646 NBP1-21502JF646
PE NBP1-21502PE
PE/Atto594 NBP1-21502PEATT594
PE/Cy5.5 NBP1-21502PECY55
PE/Cy7 NBP1-21502PECY7

Additional Ferroportin/SLC40A1 Products

Bioinformatics Tool for Ferroportin/SLC40A1 Antibody (NBP1-21502)

Discover related pathways, diseases and genes to Ferroportin/SLC40A1 Antibody (NBP1-21502). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ferroportin/SLC40A1 Antibody (NBP1-21502)

Discover more about diseases related to Ferroportin/SLC40A1 Antibody (NBP1-21502).
 

Pathways for Ferroportin/SLC40A1 Antibody (NBP1-21502)

View related products by pathway.

PTMs for Ferroportin/SLC40A1 Antibody (NBP1-21502)

Learn more about PTMs related to Ferroportin/SLC40A1 Antibody (NBP1-21502).
 

Research Areas for Ferroportin/SLC40A1 Antibody (NBP1-21502)

Find related products by research area.

Blogs on Ferroportin/SLC40A1

There are no specific blogs for Ferroportin/SLC40A1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Submit a Review

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
6
4
0
3
1
2
0
1
0

Anonymous
08/08/2018
Application: WB
Species: Mouse

Anonymous
07/12/2018
Application: WB
Species: Pig

Anonymous
04/04/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SLC40A1
Entrez
Uniprot
Novus 100% Guarantee