Ferroportin/SLC40A1 Antibody - BSA Free

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Mouse Ferroportin/SLC40A1, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human wt-FHC. Proteins of subcellular fractions isolated from livers of wt-, ms-, and LRS-treated mice (n = 4) were run on a western blot (30 ug protein/lane) and immunostained for microsomal ferroportin. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2014.00079/abstract), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with Ferroportin/SLC40A1 Antibody at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine small intestinal section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The antibody primarily developed a membranous staining pattern in the intestinal epithelial cells.

Flow Cytometry: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 Antibody NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Western blot of human intestine lysate (Molecular weight: 62.5 KDa) using Ferroportin/SLC40A1 Antibody.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Western blot of bovine adult eye using Ferroportin/SLC40A1 Antibody. Image from verified customer review.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed using (NBP1-21502; 1:1000 dilution) and GAPDH (loading control). Image from verified customer review.

Western Blot: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - HepG2 clones overexpressing heparanase showed a reduction of hepcidin expression and indices of iron loading. Two stable clones of HepG2 cells transfected with pcDNA3.1-HPA (HPA3 and HPA6) were analyzed for hepcidin expression, BMP/SMAD signaling and indices of iron status. Western blot of Ferroportin/SLC40A1 and its densitometry. The values are expressed as-dCt (for HPA mRNA) or as fold change over the control (MOCK) (for hepcidin and Id1 mRNA). The images are representative from three different analyses. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371:journal.pone.0164183), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Immunostaining for Ferroportin/SLC40A1 shows uniform reactivity on the ciliary epithelium (panel 1). Fpn is expressed mainly in NPE cells (panel 2). H&E stained section shows orientation of the ciliary epithelium with respect to the iris and AH (panel 3). Fpn is expressed on corneal epithelium (panel 4) and endothelium (panel 5). Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29859760/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine liver section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The representative image shows intense staining in the cellular membranes, whereas, a relatively milder postivity was observed in the cytoplasm of hepatocytes.

Flow Cytometry: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - Analysis using the PE conjugate of Ferroportin/SLC40A1 Antibody. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin/SLC40A1 antibody. Image from verified customer review.

Flow Cytometry: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - An intracellular stain was performed on HepG2 cells with NBP1-21502AF647 (blue) and a matched isotype control (orange, NBP2-24893AF647). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: Ferroportin/SLC40A1 Antibody - BSA Free [NBP1-21502] - An intracellular stain was performed on Hek293 cells with NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ferroportin/SLC40A1 Antibody - BSA Free Summary

• Immunogen: This Ferroportin/SLC40A1 Antibody was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]
• Localization: Cell membrane (high), Cytoplasm (low)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC40A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1.0 ug/ml. Use reported by customer review
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 ug/ml
• Application Notes: In Western blot, a band is seen at ~62 kDa.
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Ferroportin/SLC40A1 Protein (NBP1-21502PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ferroportin/SLC40A1 Antibody - BSA Free

• Ferroportin
• Ferroportin-1
• FPN1
• FPN1IREG1ferroportin 1
• HFE4
• HFE4ferroportin-1
• IREG1
• iron regulated gene 1
• Iron-regulated transporter 1
• member 3
• MST079
• MSTP079
• MTP1
• putative ferroportin 1 variant IIIB
• SLC11A3
• SLC11A3iron regulated gene 1
• SLC40A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)
• solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
• solute carrier family 40 (iron-regulated transporter), member 1
• solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(146)

Publications using NBP1-21502

• Hazlett HF Characterizing the Role of Human Macrophage Iron Metabolism in Inflammatory Lung Disease Thesis 2021-01-01
• Zhang L, Song Y, Cao K Et al. Hepcidin Based Nanocomposites for Enhanced Cancer Immunotherapy by Modulating Iron Export Mediated N6 Methyladenosine RNA Transcript Advanced Functional Materials 2021-10-07 (WB)
• Qin Y, Bai D, Tang M et al. Ketogenic diet alleviates brain iron deposition and cognitive dysfunction via Nrf2-mediated ferroptosis pathway in APP/PS1 mouse Brain research 2023-08-01 [PMID: 37164173] (WB, Mouse)
• Li X, Jiang X, Chu H et al. Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition Chinese Herbal Medicines 2020-12-01 (WB, Rat)
• Sun F, Cui L, Qian J et al. Decidual Stromal Cell Ferroptosis Associated with Abnormal Iron Metabolism Is Implicated in the Pathogenesis of Recurrent Pregnancy Loss International Journal of Molecular Sciences 2023-04-25 [PMID: 37175543] (IHC-P, WB, Human)
• Shi L, Tao Z, Zheng L et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgf?1 signaling pathway Redox Biology 2023-05-01 [PMID: 37156218] (WB, Mouse)
• Mukhopadhyay S, Encarnación-Rosado J, Lin EY et al. Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer Science advances 2023-04-21 [PMID: 37075122] (WB, Mouse)
• Nagpal A, Needham K, Lane DJR et al. Integrin ?v?3 Is a Master Regulator of Resistance to TKI-Induced Ferroptosis in HER2-Positive Breast Cancer Cancers 2023-02-14 [PMID: 36831558] (WB, Mouse)
• Xie J, Lv H, Liu X et al. Nox4-and Tf/TfR-mediated peroxidation and iron overload exacerbate neuronal ferroptosis after intracerebral hemorrhage: Involvement of EAAT3 dysfunction Free radical biology & medicine 2023-02-18 [PMID: 36805044] (WB, Rat)
• Zhao J, Jia Y, Mahmut D et al. Human hematopoietic stem cell vulnerability to ferroptosis Cell 2023-02-16 [PMID: 36803603]
• Santhakumar S, Athiyarath R, Cherian AG et al. Impact of maternal iron deficiency anemia on fetal iron status and placental iron transporters in human pregnancy Blood cells, molecules & diseases 2023-01-27 [PMID: 36725474] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Han Y, Luo Z, Yue ZG et al. The tendency of anemia of inflammation in periodontal diseases Clinical science (London, England : 1979) 2023-02-14 [PMID: 36705427] (WB, Mouse)
• Joffin N, Gliniak CM, Funcke JB et al. Adipose tissue macrophages exert systemic metabolic control by manipulating local iron concentrations Nature metabolism 2022-11-01 [PMID: 36329217] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Wang H, Dou M, Li J et al. Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation In vitro cellular & developmental biology. Animal 2022-09-30 [PMID: 36178582] (ICC/IF, Rat)
• Song Y, Yang N, Si H et al. Iron overload impairs renal function and is associated with vascular calcification in rat aorta Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2022-09-30 [PMID: 36178540] (ICC/IF, Rat)
• Li L, Wang K, Jia R et al. Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation Redox biology 2022-08-23 [PMID: 36029649] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Wang LJ, Zhao GP, Wang XF et al. Glycochenodeoxycholate Affects Iron Homeostasis via Up-Regulating Hepcidin Expression Nutrients 2022-08-02 [PMID: 35956351] (WB, Human, Mouse)
  
  Details:
  Dilution used 1:1000
• Doan LH, Chu LW, Huang ZY et al. Virofree, an Herbal Medicine-Based Formula, Interrupts the Viral Infection of Delta and Omicron Variants of SARS-CoV-2 Frontiers in pharmacology 2022-07-04 [PMID: 35860023]
• Yuan B, Zhao XD, Shen JD et al. Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage Oxidative medicine and cellular longevity 2022-07-09 [PMID: 35855863] (WB, Mouse)
• Wang D, Zhang S, Ge X et al. Mesenchymal stromal cell treatment attenuates repetitive mild traumatic brain injury-induced persistent cognitive deficits via suppressing ferroptosis Journal of neuroinflammation 2022-07-14 [PMID: 35836233] (WB, Mouse)
  
  Details:
  1:1000 dilution
• Hu CB, Jiang H, Yang Y et al. DL-3-n-butylphthalide alleviates motor disturbance by suppressing ferroptosis in a rat model of Parkinson's disease Neural Regeneration Research 2023-01-01 [PMID: 35799542] (WB, Rat)
• Zhang H, Jamieson KL, Grenier J et al. Myocardial Iron Deficiency and Mitochondrial Dysfunction in Advanced Heart Failure in Humans Journal of the American Heart Association 2022-06-07 [PMID: 35656974] (WB, Human)
• Wang C, Zhang W, Xu W et al. AMP-activated protein kinase alpha 1 phosphorylates PHD2 to maintain systemic iron homeostasis Clinical and translational medicine 2022-05-01 [PMID: 35538889] (WB, Mouse)
• Jiang H, Zhang X, Yang W et al. Ferrostatin-1 Ameliorates Liver Dysfunction via Reducing Iron in Thioacetamide-induced Acute Liver Injury in Mice Frontiers in Pharmacology 2022-04-12 [PMID: 35496274] (WB, Mouse)
• Park WR, Choi B, Kim YJ et al. Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes International journal of molecular sciences 2022-03-25 [PMID: 35408955] (WB, Mouse)
• Soliman S, El-Labbad E, Abu-Qiyas A et al. Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis Frontiers in Microbiology 2022-03-21 [PMID: 35387075] (WB, Human)
• Labomascus BB Impact Of Maternal Pre-pregnancy Obesity On Placental Iron Transporter Protein Expression Thesis
• Jiang H, Muir R, Gonciarz R et al. Exploiting KRAS-Driven Ferroaddiction in Cancer Through Ferrous Iron-Activatable Drug Conjugates (FeADC) J Exp Med 2022-03-09 [PMID: 35262628]
• FernAndez-GarcIa V, GonzAlez-Ramos S, AvendaNo-Ortiz J Et al. NOD1 splenic activation confers ferroptosis protection and reduces macrophage recruitment under pro-atherogenic conditions Biomed Pharmacother 2022-03-05 [PMID: 35247718] (FLOW, Mouse)
  
  Details:
  Citation using the PE version of this antibody.
• Wyart E, Hsu MY, Sartori R et al. Iron supplementation is sufficient to rescue skeletal muscle mass and function in cancer cachexia EMBO reports 2022-02-24 [PMID: 35199910] (WB, Human)
• Sato T, Shapiro JS, Chang HC et al. Aging is associated with increased brain iron through cortex-derived hepcidin expression eLife 2022-01-11 [PMID: 35014607] (WB, Mouse)
• Arita A, Kita I, Shinoda S Internalization and Decrease of Duodenal DMT1 Involved in Transient Suppression of Iron Uptake in Short-Acting Mucosal Block Biological trace element research 2022-01-08 [PMID: 34997531]
• Deng Q, Yang S, Sun L et al. A detrimental role of NLRP6 in host iron metabolism during Salmonella infection Redox biology 2021-12-18 [PMID: 34942528] (WB, Mouse)
• Sp N, Kang DY, Jo ES Et al. Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells Frontiers in oncology 2021-11-03 [PMID: 34804985] (WB, Human)
• Kang DY, Sp N, Jo ES Et al. New Insights into the Pivotal Role of Iron/Heme Metabolism in TLR4/NF- kappa B Signaling-Mediated Inflammatory Responses in Human Monocytes Cells 2021-09-27 [PMID: 34685529] (WB, Human)
• Han M, BOhlke M, Maher T, Kim J Alcohol exposure increases manganese accumulation in the brain and exacerbates manganese-induced neurotoxicity in mice Archives of toxicology 2021-12-01 [PMID: 34590183] (WB, Mouse)
• Liu K, Liu J, Zou B et al. Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice Cellular and molecular gastroenterology and hepatology 2021-09-22 [PMID: 34562639] (WB, Mouse)
• VAsquez-Trincado C, Patel M, Sivaramakrishnan A et al. Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation Human molecular genetics 2021-09-22 [PMID: 34550363]
• Konieczny P, Mukherjee S, Stepniak-Konieczna E Et al. Cyclic mismatch binding ligands interact with disease-associated CGG trinucleotide repeats in RNA and suppress their translation Nucleic acids research 2021-08-06 [PMID: 34358321] (WB)
• Wang X, Wang Z, Cao J Et Al. Melatonin Alleviates Acute Sleep Deprivation-Induced Memory Loss in Mice by Suppressing Hippocampal Ferroptosis Frontiers in Pharmacology 2021-07-16 [PMID: 34335271] (WB)
• Bailey Dk, Clark W, Kosman Dj The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells PloS one 2021-07-26 [PMID: 34310628] (WB)
• Zhao S, Wang X, Zheng X Et Al. Iron deficiency exacerbates cisplatin- or rhabdomyolysis-induced acute kidney injury through promoting iron-catalyzed oxidative damage Free radical biology & medicine 2021-07-21 [PMID: 34298093] (WB)
• Argenziano M, Tortora C, Paola AD et al. Eltrombopag and its iron chelating properties in pediatric acute myeloid leukemia Oncotarget 2021-07-06 [PMID: 34262648] (WB)
• Chaudhary S, Ashok A, Wise AS et al. Upregulation of brain hepcidin in prion diseases Prion 2021-12-01 [PMID: 34224321] (WB)
• Wei D, Duan Bx, Wang Cy, Cao P MicroRNA-302a-3p induces ferroptosis of non-small cell lung cancer cells via targeting ferroportin Free radical research 2021-06-28 [PMID: 34181495]
• Zhang Z, Funcke JB, Zi Z et al. Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity Cell metabolism 2021-06-18 [PMID: 34174197]
• Schwartz AJ, Goyert JW, Solanki S et al. Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells Nature metabolism 2021-06-21 [PMID: 34155415]
• Mohammad G, Matakidou A, Robbins PA, Lakhal-Littleton S The kidney hepcidin/ferroportin axis controls iron reabsorption and determines the magnitude of kidney and systemic iron overload Kidney international 2021-05-12 [PMID: 33991530] (IHC-P, WB, Mouse)
• Asperti M, Bellini S, Grillo E et al. H-ferritin suppression and pronounced mitochondrial respiration make Hepatocellular Carcinoma cells sensitive to RSL3-induced ferroptosis Free radical biology & medicine 2021-04-20 [PMID: 33892112]
• Lu S, Song Y, Luo R et al. Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo Oxidative medicine and cellular longevity 2021-02-10 [PMID: 33628376] (WB, IF/IHC)
• Derbis M, Kul E, Niewiadomska D et al. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats Nature communications 2021-02-24 [PMID: 33627639] (WB, Human)
• Deng Q, Yang S, Sun L, et al. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin-ferroportin axis Gut microbes 2021-01-21 [PMID: 33475464] (WB, Mouse)
• Li J, Liu J, Xu Y, et al. Tumor heterogeneity in autophagy-dependent ferroptosis Autophagy 2021-01-15 [PMID: 33404288]
• Li Y, Zeng X, Lu D, et al. Erastin induces ferroptosis via ferroportin-mediated iron accumulation in endometriosis Human reproduction (Oxford, England) 2020-12-30 [PMID: 33378529]
• FernAndez-MendIvil C, Luengo E, Trigo-Alonso P et al. Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism Redox Biol 2020-11-06 [PMID: 33212416] (WB)
• Yamaguchi H, Honda S, Torii S et al. Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration Nat Commun 2020-10-20 [PMID: 33082312] (WB, ICC/IF, Human)
• Verma N, Vinik Y, Saroha A et al. Synthetic lethal combination targeting BET uncovered intrinsic susceptibility of TNBC to ferroptosis Sci. Adv. 2020-08-01 [PMID: 32937365]
• Yang G, Qian C, Zhang C et al. Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats Transl Res 2020-09-12 [PMID: 32932001] (WB, Rat)
• Renassia C, Louis S, Cuvellier S et al. Neutrophils from hereditary hemochromatosis patients are protected from iron excess and are primed Blood Adv 2020-08-25 [PMID: 32810223] (WB, Human)
• Lin PL, Tang HH, Wu SY et al. Saponin Formosanin C-induced Ferritinophagy and Ferroptosis in Human Hepatocellular Carcinoma Cells Antioxidants (Basel) 2020-07-29 [PMID: 32751249] (WB, Human)
• Kim HY, Kwon WY, Park JB et al. Hepatic STAMP2 mediates recombinant FGF21-induced improvement of hepatic iron overload in nonalcoholic fatty liver disease FASEB J. 2020-07-28 [PMID: 32721044]
• Pease NA, Shephard MS, Sertorio M et al. DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages Cancers (Basel) 2020-07-17 [PMID: 32708944] (WB, Mouse)
• Lee J, You JH, Shin D, Roh JL Inhibition of Glutaredoxin 5 predisposes Cisplatin-resistant Head and Neck Cancer Cells to Ferroptosis Theranostics 2020-06-19 [PMID: 32685019] (WB, Human)
• Tsuji Y Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SLC40A1 (ferroportin) PLoS ONE 2020-07-09 [PMID: 32645092] (WB, KD, Human)
• Hazlett HF, Hampton TH, Aridgides DS et al. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival Sci Rep 2020-07-02 [PMID: 32616918]
• Li S, Qian ZM, Xu G et al. Hepatocyte growth factor protects PC12 cells against OGD/R-induced injury by reducing iron Biosci. Rep. 2020-04-30 [PMID: 32186328] (WB, Rat)
• Gonciarz R, Jiang H, Hugelshofter C et al. In vivo Bioluminescence Imaging of Labile Iron in Xenograft Models and Liver Using FeAl-1, an Iron-Activatable Form of D-Luciferin SSRN Electronic Journal 2022-11-20
• KAmmerer, L, Mohammad, G Et al. Fetal liver hepcidin secures iron stores in utero. Blood 2020-09-24 [PMID: 32542311] (WB, Mouse)
• Hao Z Understanding Heart Failure in Patients with Dilated Cardiomyopathy: A Direct Tissue Analysis from Explanted Human Hearts Thesis 2022-01-01 (WB, Human)
• Kammerer L, Mohammad G, Wolna M, Robbins P Fetal liver hepcidin supports acquisition of iron stores in utero BioRxiv 2019-10-09 [PMID: 32542311] (IHC-Fr, Mouse)
• Wang R, Liu X, Wu J et al. Role of microRNA-122 in microcystin-leucine arginine-induced dysregulation of hepatic iron homeostasis in mice Environ. Toxicol. 2020-03-14 [PMID: 32170997]
• Sheng Y, Chen Yj, Qian Zm et Al. Cyclophosphamide induces a significant increase in iron content in the liver and spleen of mice Hum Exp Toxicol 2020-03-04 [PMID: 32129080]
• Schnetz M, Meier JK, Rehwald C et al. The Disturbed Iron Phenotype of Tumor Cells and Macrophages in Renal Cell Carcinoma Influences Tumor Growth Cancers (Basel) 2020-02-25 [PMID: 32106629] (ICC/IF, Human)
• Zhao Y, Guo H, Li Q et al. TREM-1 Aggravates Diabetic Associated Cognitive Impairment by Inducing Iron Deposition in Microglia via ERS Research Square 2023-04-13 (WB, Mouse)
• Singh KS, Leu JI, Barnoud T et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin Nat Commun 2020-01-24 [PMID: 31980600] (WB)
• Neves JV, Gomes AC, Costa DM et al. A role for hepcidin in the anemia caused by Trypanosoma brucei infection Haematologica 2020-01-09 [PMID: 31919087] (WB, Mouse)
• Yasumura S, Naito Y, Okuno K et al. Effects of Heterozygous TfR1 (Transferrin Receptor 1) Deletion in Pathogenesis of Renal Fibrosis in Mice Hypertension 2019-12-16 [PMID: 31838906]
• Ashok A, Chaudhary S, McDonald D, et al. Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications. Exp Eye Res 2019-12-04 [PMID: 31811823] (IF, Bovine, Human)
• Villalpando-Rodriguez G, Blankstein A, Konzelman C, Gibson S Lysosomal Destabilizing Drug Siramesine and the Dual Tyrosine Kinase Inhibitor Lapatinib Induce a Synergistic Ferroptosis through Reduced Heme Oxygenase-1 (HO-1) Levels Oxid Med Cell Longev. 2019-09-17 [PMID: 31636810]
• Tamiya H, Urushihara N, Shizuma K et al. SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-?B- and PRMT5-mediated PGC1? reduction bioRxiv 2023-04-17 (WB, Mouse)
• Chen X, Li D, Sun Hy et Al. Relieving ferroptosis may partially reverse neurodegeneration of the auditory cortex FEBS J. 2020-02-28 [PMID: 32112499] (WB)
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• Zhang MW, Zhao P, Yung WH et al. Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice Aging (Albany NY) 2018-12-16 [PMID: 30555055] (WB, Mouse)
• Miyazawa M, Bogdan AR, Tsuji Y. Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2. Cell Chem Biol. 2018-10-25 [PMID: 30449675] (WB, Human)
• Silva JM, Rodrigues S, Sampaio-Marques B et al. Dysregulation of autophagy and stress granule-related proteins in stress-driven Tau pathology. Cell Death Differ. 2018-11-15 [PMID: 30442948] (WB, Mouse)
• Shin D, Kim EH, Lee J, Roh JL. Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer. Free Radic. Biol. Med. 2018-10-16 [PMID: 30339884] (Mouse)
• Samba-Mondonga M, Calve A, Mallette FA, Santos MM. MyD88 Regulates the Expression of SMAD4 and the Iron Regulatory Hormone Hepcidin. Front Cell Dev Biol 2018-08-31 [PMID: 30234111] (WB, Human)
• Ramakrishnan L, Pedersen SL, Toe QK et al. The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells Sci Rep 2018-08-28 [PMID: 30154413] (IF/IHC, Human)
• Sajja RK, Kaisar MA, Vijay V et al. In Vitro Modulation of Redox and Metabolism Interplay at the Brain Vascular Endothelium: Genomic and Proteomic Profiles of Sulforaphane Activity Sci Rep 2018-08-23 [PMID: 30139948] (WB, ICC/IF, Mouse)
• Ma J, Zhang FL, Zhou G et al. Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6-/- Neurons and Astrocytes Treated with Lipopolysaccharides Neurochem Res. [PMID: 29923037] (WB, Mouse)
• Kaisar MA, Sivandzade F, Bhalerao A, Cucullo L Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: In vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure Neurosci Lett. 2018-08-23 [PMID: 29879439] (WB, Mouse)
• Ashok A, Karmakar S, Chandel R et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission Exp. Eye Res. 2018-05-31 [PMID: 29859760] (ICC/IF, Human)
• Lim D, Kim KS, Jeong JH et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages Nat Commun 2018-05-29 [PMID: 29844422] (WB, Mouse)
• Blanchette-Farra N, kita D, konstorum A et al. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer. Oncogene. 2018-07-01 [PMID: 29695834] (WB, Human)
• Broide E, Reifen R, Matalon S et al. Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia. J Diabetes Res 2018-03-06 [PMID: 29693022] (WB, Human)
• Puy V, Darwiche W, Trudel S et al. Predominant role of microglia in brain iron retention in Sanfilippo syndrome, a pediatric neurodegenerative disease. Glia 2018-04-06 [PMID: 29624734] (WB, Mouse)
• McCarthy RC, SoSa JC, Gardeck AM et al. Inflammation-induced Iron Transport and Metabolism by Brain Microglia. J. Biol. Chem. 2018-04-02 [PMID: 29610275] (Mouse)
• Mertens C, Mora J, Oren B et al. Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment Oncoimmunology 2017-12-22 [PMID: 29399416] (FLOW, Mouse)
• Zhabyeyev P, Das SK, Basu R et al. TIMP3 deficiency exacerbates iron-overload mediated cardiomyopathy and liver disease Am. J. Physiol. Heart Circ. Physiol. 2018-01-26 [PMID: 29373036] (WB, Mouse)
• Raggi C, Gammella E, Correnti M et al. Dysregulation of Iron Metabolism in Cholangiocarcinoma Stem-like Cells. Sci Rep 2017-12-15 [PMID: 29247214] (WB, Human)
• Zhang FL, Hou HM, Yin ZN et al. Impairment of Hepcidin Upregulation by Lipopolysaccharide in the Interleukin-6 Knockout Mouse Brain Front Mol Neurosci 2017-11-07 [PMID: 29163042] (WB, Mouse)
• Baba Y, Higa JK, Shimada BK et al. Protective Effects of the Mechanistic Target of Rapamycin against Excess Iron and Ferroptosis in Cardiomyocytes Am. J. Physiol. Heart Circ. Physiol. 2017-11-10 [PMID: 29127238] (Mouse)
• Neves JV, Ramos MF, Moreira AC et al. Hamp1 but not Hamp2 regulates ferroportin in fish with two functionally distinct hepcidin types Sci Rep. 2017-11-01 [PMID: 29093559] (Mouse)
• Zhang W, Lv T, Huang J, Ou X. Type 4B hereditary hemochromatosis associated with a novel mutation in the SLC40A1 gene: A case report and a review of the literature Medicine (Baltimore) 2017-09-01 [PMID: 28930842] (IF/IHC, Human)
• Pasricha SR, Lim PJ, Duarte TL et al. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun 2017-09-01 [PMID: 28864822] (WB, Human)
• Dietrich P, Johnson IM, Alli S, Dragatsis I. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis PLoS Genet. 2017-07-01 [PMID: 28715425] (WB, Mouse)
• Basuli D, Tesfay L, Deng Z et al. Iron addiction: a novel therapeutic target in ovarian cancer Oncogene 2017-07-20 [PMID: 28319068] (WB, Human)
• Fader KA, Nault R, Kirby MP et al. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBalpha/b activation in aryl hydrocarbon receptor-elicited hepatotoxicity. Toxicol. Appl. Pharmacol. 2017-02-16 [PMID: 28213091]
• Mertens C, Akam EA, Rehwald C et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS ONE. 2016-11-02 [PMID: 27806101] (FLOW, Human)
• Zhang YT, Li FM, Guo YZ et al. (Z)-ligustilide increases ferroportin1 expression and ferritin content in ischemic SH-SY5Y cells. Eur. J. Pharmacol. 2016-10-24 [PMID: 27789221] (WB)
• Gasperini L, Meneghetti E, Legname G, Benetti F. In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. Front Neurosci. 2016-10-12 [PMID: 27729845] (WB, Mouse)
• Asperti M, Stuemler T, Poli M et al. Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation. PLoS One 2016-10-06 [PMID: 27711215] (WB)
• Kim DK, Kim YH, Jung YS et al. Orphan nuclear receptor SHP regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression. Sci Rep 2016-09-30 [PMID: 27688041] (WB)
• Marques O, Porto G, Rema A et al. Local iron homeostasis in the breast ductal carcinoma microenvironment BMC Cancer 2016-03-05 [PMID: 26944411] (IHC-P, Human)
• Theurl M, Song D, Clark E et al. Mice with hepcidin-resistant ferroportin accumulate iron in the retina. FASEB J 2016-02-01 [PMID: 26506980]
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  Details:
  WB with Ferroportin 1 antibody on whole-cell lysates of immortalized WT and SIRT3 KO MEFs showing a decreased FPN1 protein expression in SIRT3 KO compared with WT cells (Figure 3e and Supplementary Figure S3f).
• Theurl M, Nairz M, Schroll A et al. Hepcidin as a predictive factor and therapeutic target in erythropoiesis-stimulating agent treatment for anemia of chronic disease in rats. Haematologica. 2014-06-03 [PMID: 24895335] (IHC-P, WB, Rat)
  
  Details:
  Formalin fixed and paraffin-wax embedded rat (female Lewis rats) spleen and duodenum tissue sections were immunostained with 1:200 dilutionof rabbit anti-Fp1 antibdoy (NBP1-21502) in PBS/1% BSA for 1h at RT followed by donkey anti rabbit Alexa 555 for immunofluorescence detection ( Supplemental Figure S4 - Splenic Fp1 and Supplemental Figure S5 - Duodenal Fp1 immunofluorescence in ACD rats treated with ESA and/or LDN-193189). Antibody also used for WB of FP1 in spleen (Figure 3A) and liver of rats (Supplemental Figure S3).
• Vercellotti Gregory M, Khan Fatima B, Nguyen Julia et al. H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice. Front Pharmacol. 2014-04-17 [PMID: 24860503] (WB, Mouse)
• Scheers NM, Almgren AB, Sandberg AS. Proposing a Caco-2/HepG2 cell model for in vitro iron absorption studies. The Journal of Nutritional Biochemistry 2014-03-19 [PMID: 24746839] (WB, Human)
• Kim DK, Jeong JH, Lee JM et al. Inverse agonist of estrogen-related receptor [gamma] controls Salmonella typhimurium infection by modulating host iron homeostasis. Nature Medicine 2014-03-23 [PMID: 24658075] (WB, Mouse)
• Scheers N, Sandberg AS. Iron Transport through Ferroportin Is Induced by Intracellular Ascorbate and Involves IRP2 and HIF2alpha. Nutrients 2014-01-03 [PMID: 24394537] (WB, Human)
• Diaz V, Gammella E, Recalcati S et al. Liver iron modulates hepcidin expression during chronically elevated erythropoiesis in mice. Hepatology 2013-06-06 [PMID: 23744538] (WB, Mouse)
• Choi JS, Koh IU, Lee HJ et al. Effects of excess dietary iron fat on glucose lipid metabolism. J Nutr Biochem 2013-05-02 [PMID: 23643521] (WB, Mouse)
• Bayeva M, Khechaduri A, Puig S et al. mTOR Regulates Cellular Iron Homeostasis through Tristetraprolin Cell Metab 2012-10-23 [PMID: 23102618] (WB, Mouse)
• Vanoaica L et al. Intestinal ferritin h is required for an accurate control of iron absorption. Cell Metab 12(3):273-82. 2010-01-01 [PMID: 20816093] (WB, Mouse)

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (7)

Average Rating: 4.7

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Bovine, Pig.

reviewed by: Ranjana Ravikumar WB Mouse 08/08/2018

Summary

• Application: Western Blot
• Sample Tested: Adult pancreas
• Species: Mouse
• Lot: F-1

reviewed by: Peng Ji WB Pig 07/12/2018

Summary

• Application: Western Blot
• Sample Tested: duodenal mucosa
• Species: Pig
• Lot: F-1

reviewed by: Ajay Ashok IHC-P Human 04/04/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult eye and muscle tissue (labeled E and M, respectively)
• Species: Human
• Lot: E-2

Comments

• Comments: 1:100

reviewed by: Ajay Ashok WB Bovine 04/04/2018

Summary

• Application: Western Blot
• Sample Tested: Adult eye
• Species: Bovine
• Lot: E-2

Comments

• Comments: 1:1000

reviewed by: Ranjana Ravikumar IHC-P Bovine 10/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: eye
• Species: Bovine
• Lot: E-2

Comments

• Comments: use at 1:100

reviewed by: Ajay Ashok IHC Mouse 06/27/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Verified Customer WB Human 06/13/2017

Summary

• Application: Western Blot
• Sample Tested: Human microglia cell lysate
• Species: Human

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 5 of 5 FAQs).

1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
   • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
2. Would it be possible to conjugate this antibody to PE or PerCP?
   • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
   • The immunogen used for this Ferroportin/SLC40A1 Antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.
4. I can’t get positive a result in IHC-P using NBP1-21502 (tissue: atherosclerosis hemorrhage plaque). PV-6001 and DAB is working. Can you make optimization suggestions on how to get this antibody to work?
   • Optimization could really make a difference as we have seen some staining with the same antibody on frozen section. The main thing would be to focus on antigen retrieval step and the buffer used. For the antigen retrieval method, we would suggest an increase to at least 10-20 minutes to the pressure cooker step of the HIER method; the duration of the retrieval step can greatly determine if the antigen will be exposed or not for the antigen binding site of the antibody to recognize the protein. Also, I the incubation time for peroxidase blocking time could be reduced from 30min to 15 min, as we have mentioned. Since this is a TMP, permeabilization reagents should be used for the experiment. You can review what we have available on our site in terms of our general protocols:HIER and IHC-P​
5. We are having difficulty producing a clean blot, i.e. when we probe our western blots with the recommended concentration of 1.0 ug/ml, we are seeing multiple bands. The order of the molecular weight ladder we used is as followed: 250, 130, 100, 70, 55, 35, 25, 15, and 10 kDa. Are you able to indicate what band may be ferroportin.?
   • There is a western blot protocol specific to this antibody that can be found on the datasheet; it differs from your protocol quite a lot. I would give this protocol a try and see if you get better results.Although I am uncertain of your blocking method, we find that BSA and PVDF can give high non-specific background so the nitrocellulose and NFDM are recommend for this antibody. In addition, there seems to be no protein expression of the SLC40A1 in liver or spleen based on Human Protein Atlas.https://www.proteinatlas.org/ENSG00000138449-SLC40A1/tissueA good positive control might be bone marrow.

Bioinformatics

• Gene Symbol: SLC40A1
• Entrez:
  • 30061(Human)
  • 53945(Mouse)
• Uniprot:
  • Q9NP59()