Ferroportin/SLC40A1 Antibody - BSA Free

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Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Western blot of human intestine lysate (Molecular weight: 62.5 KDa) using Ferroportin/SLC40A1 Antibody.
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed using (NBP1-21502; 1:1000 dilution) and GAPDH (loading ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on Hek293 cells with NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 Antibody NBP1-21502 (blue) and a matched isotype control NBP2-24891 (orange). ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Mouse Ferroportin/SLC40A1, 5-aminolevulinic acid synthase (ALAS), and ferritin light chain (FLC) proteins are increased in sickle mice overexpressing human ...read more
Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with Ferroportin/SLC40A1 Antibody at ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine small intestinal section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Western blot of bovine adult eye using Ferroportin/SLC40A1 Antibody. Image from verified customer review.
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 clones overexpressing heparanase showed a reduction of hepcidin expression and indices of iron loading. Two stable clones of HepG2 cells transfected with ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Detection of Ferroportin/SLC40A1 protein in murine liver section using Ferroportin/SLC40A1 Antibody at a dilution of 1:200. The representative ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of Ferroportin/SLC40A1 Antibody. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with NBP1-21502AF647 (blue) and a matched isotype control (orange, NBP2-24893AF647). Cells were fixed with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Ferroportin/SLC40A1 Antibody - BSA Free Summary

Immunogen
This Ferroportin/SLC40A1 Antibody was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]
Localization
Cell membrane (high), Cytoplasm (low)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC40A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 ug/ml
Application Notes
In Western blot, a band is seen at ~62 kDa.
Theoretical MW
62.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Ferroportin/SLC40A1 Protein (NBP1-21502PEP)
Reviewed Applications
Read 7 Reviews rated 4.7
using
NBP1-21502 in the following applications:

Publications
Read Publications using
NBP1-21502 in the following applications:

  • 1 publication
  • 2 publications
  • 4 publications
  • IF
    1 publication
  • IHC
    3 publications
  • 1 publication
  • 4 publications
  • KD
    1 publication
  • WB
    82 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Antibody tested in Flow Cytometry by verified customer through our Innovator's Reward Program.

Alternate Names for Ferroportin/SLC40A1 Antibody - BSA Free

  • Ferroportin
  • Ferroportin-1
  • FPN1
  • FPN1IREG1ferroportin 1
  • HFE4
  • HFE4ferroportin-1
  • IREG1
  • iron regulated gene 1
  • Iron-regulated transporter 1
  • member 3
  • MST079
  • MSTP079
  • MTP1
  • putative ferroportin 1 variant IIIB
  • SLC11A3
  • SLC11A3iron regulated gene 1
  • SLC40A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)
  • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
  • solute carrier family 40 (iron-regulated transporter), member 1
  • solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(121)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Porcine.

We have publications tested in 9 applications: FLOW, Flow, ICC/IF, IF, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
FLOW
(1)
Flow
(2)
ICC/IF
(4)
IF
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(4)
KD
(1)
WB
(82)
All Applications
Filter By Species
Human
(34)
Mouse
(44)
Rat
(4)
Bovine
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using NBP1-21502 Applications Species
Jiang H, Zhang X, Yang W et al. Ferrostatin-1 Ameliorates Liver Dysfunction via Reducing Iron in Thioacetamide-induced Acute Liver Injury in Mice Frontiers in Pharmacology Apr 12 2022 [PMID: 35496274] (WB, Mouse) WB Mouse
Park WR, Choi B, Kim YJ et al. Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes International journal of molecular sciences Mar 25 2022 [PMID: 35408955] (WB, Mouse) WB Mouse
Soliman S, El-Labbad E, Abu-Qiyas A et al. Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis Frontiers in Microbiology Mar 21 2022 [PMID: 35387075] (WB, Human) WB Human
Wyart E, Hsu MY, Sartori R et al. Iron supplementation is sufficient to rescue skeletal muscle mass and function in cancer cachexia EMBO reports Feb 24 2022 [PMID: 35199910] (WB, Human) WB Human
Sato T, Shapiro JS, Chang HC et al. Aging is associated with increased brain iron through cortex-derived hepcidin expression eLife Jan 11 2022 [PMID: 35014607] (WB, Mouse) WB Mouse
Arita A, Kita I, Shinoda S Internalization and Decrease of Duodenal DMT1 Involved in Transient Suppression of Iron Uptake in Short-Acting Mucosal Block Biological trace element research Jan 8 2022 [PMID: 34997531]
Deng Q, Yang S, Sun L et al. A detrimental role of NLRP6 in host iron metabolism during Salmonella infection Redox biology Dec 18 2021 [PMID: 34942528] (WB, Mouse) WB Mouse
Sp N, Kang DY, Jo ES Et al. Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells Frontiers in oncology Nov 3 2021 [PMID: 34804985] (WB, Human) WB Human
Kang DY, Sp N, Jo ES Et al. New Insights into the Pivotal Role of Iron/Heme Metabolism in TLR4/NF- kappa B Signaling-Mediated Inflammatory Responses in Human Monocytes Cells Sep 27 2021 [PMID: 34685529] (WB, Human) WB Human
Han M, BOhlke M, Maher T, Kim J Alcohol exposure increases manganese accumulation in the brain and exacerbates manganese-induced neurotoxicity in mice Archives of toxicology Dec 1 2021 [PMID: 34590183] (WB, Mouse) WB Mouse
Show All 121 Publications.

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (7) 4.77

Average Rating: 4.7
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Bovine, Pig.

Reviews using NBP1-21502:
Filter by Applications
Western Blot
(4)
Immunohistochemistry-Paraffin
(2)
Immunohistochemistry
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Bovine
(2)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Ranjana Ravikumar
Western Blot Mouse 08/08/2018
View

Summary

ApplicationWestern Blot
Sample TestedAdult pancreas
SpeciesMouse
LotF-1
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Peng Ji
Western Blot Pig 07/12/2018
View

Summary

ApplicationWestern Blot
Sample Testedduodenal mucosa
SpeciesPig
LotF-1
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Ajay Ashok
Immunohistochemistry-Paraffin Human 04/04/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult eye and muscle tissue (labeled E and M, respectively)
SpeciesHuman
LotE-2

Comments

Comments1:100
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Ajay Ashok
Western Blot Bovine 04/04/2018
View

Summary

ApplicationWestern Blot
Sample TestedAdult eye
SpeciesBovine
LotE-2

Comments

Comments1:1000
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Ranjana Ravikumar
Immunohistochemistry-Paraffin Bovine 10/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedeye
SpeciesBovine
LotE-2

Comments

Commentsuse at 1:100
Immunohistochemistry Ferroportin/SLC40A1 NBP1-21502
Enlarge
3
reviewed by:
Ajay Ashok
Immunohistochemistry Mouse 06/27/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse brain
SpeciesMouse
Western Blot Ferroportin/SLC40A1 NBP1-21502
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 5 of 5 FAQs).

  1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
    • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
  2. Would it be possible to conjugate this antibody to PE or PerCP?
    • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
  3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
    • The immunogen used for this Ferroportin/SLC40A1 Antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.
  4. I can’t get positive a result in IHC-P using NBP1-21502 (tissue: atherosclerosis hemorrhage plaque). PV-6001 and DAB is working. Can you make optimization suggestions on how to get this antibody to work?
    • Optimization could really make a difference as we have seen some staining with the same antibody on frozen section. The main thing would be to focus on antigen retrieval step and the buffer used. For the antigen retrieval method, we would suggest an increase to at least 10-20 minutes to the pressure cooker step of the HIER method; the duration of the retrieval step can greatly determine if the antigen will be exposed or not for the antigen binding site of the antibody to recognize the protein. Also, I the incubation time for peroxidase blocking time could be reduced from 30min to 15 min, as we have mentioned. Since this is a TMP, permeabilization reagents should be used for the experiment. You can review what we have available on our site in terms of our general protocols:HIER: https://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.htmlIHC-P: https://www.novusbio.com/support/support-by-application/immunohistochemistry-paraffin/protocol.html
  5. We are having difficulty producing a clean blot, i.e. when we probe our western blots with the recommended concentration of 1.0 ug/ml, we are seeing multiple bands. The order of the molecular weight ladder we used is as followed: 250, 130, 100, 70, 55, 35, 25, 15, and 10 kDa. Are you able to indicate what band may be ferroportin.?
    • There is a western blot protocol specific to this antibody that can be found on the datasheet; it differs from your protocol quite a lot. I would give this protocol a try and see if you get better results.https://resources.novusbio.com/protocols/Western-Blot-Protocol-for-Ferroportin-1-Antibody-(NBP1-21502).pdfAlthough I am uncertain of your blocking method, we find that BSA and PVDF can give high non-specific background so the nitrocellulose and NFDM are recommend for this antibody. In addition, there seems to be no protein expression of the SLC40A1 in liver or spleen based on Human Protein Atlas.https://www.proteinatlas.org/ENSG00000138449-SLC40A1/tissueA good positive control might be bone marrow.

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Recent Reviews

4.7
5
6
4
0
3
1
2
0
1
0

Ranjana Ravikumar
08/08/2018
Application: Western Blot
Species: Mouse

Peng Ji
07/12/2018
Application: Western Blot
Species: Pig

Ajay Ashok
04/04/2018
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol SLC40A1
Entrez
Uniprot