Ferroportin/SLC40A1 Antibody

Images

 
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Duodenal mucosa samples from weanling pigs with iron replete or iron deficient status were probed for FPN1 (NBP1-21502; 1:1000 dilution) and GAPDH (loading ...read more
Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin/SLC40A1 ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine small intestinal section using Ferroportin 1 antibody at a dilution of 1:200. The antibody ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 Antibody NBP1-21502 and a matched isotype control. Cells were fixed with 4% PFA ...read more
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Human intestine lysate.
Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Bovine adult eye. Image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin 1 at 5.0 ug/ml ...read more
Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine liver section using Ferroportin 1 antibody at a dilution of 1:200. The representative image ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Histogram of Ferroportin staining of human spleen macrophages using Alexa Fluor 700 conjugated Ferroportin antibody (red, catalog# NBP1-21502AF700) and isotype ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of NBP1-21502. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin ...read more
Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF647 (blue) and a matched isotype control NBP2-24893AF647 ...read more
Flow (Intracellular): Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF700 (blue) and a matched isotype control (orange). ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, BvSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Ferroportin/SLC40A1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot# Q9NP59]
Localization
Cell membrane (high), Cytoplasm (low)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC40A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
Application Notes
This Ferroportin 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62 kDa.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Small Intestine Lysate (NB820-59255)
Control Peptide
Ferroportin/SLC40A1 Peptide (NBP1-21502PEP)
Reviewed Applications
Read 7 Reviews rated 4.7
using
NBP1-21502 in the following applications:

Publications
Read Publications using
NBP1-21502 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Antibody tested in Flow Cytometry by verified customer through our Innovator's Reward Program.

Alternate Names for Ferroportin/SLC40A1 Antibody

  • Ferroportin
  • Ferroportin-1
  • FPN1
  • FPN1IREG1ferroportin 1
  • HFE4
  • HFE4ferroportin-1
  • IREG1
  • Iron-regulated transporter 1
  • member 3
  • MST079
  • MTP1
  • putative ferroportin 1 variant IIIB
  • SLC11A3
  • SLC11A3iron regulated gene 1
  • SLC40A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)
  • solute carrier family 40 (iron-regulated transporter), member 1
  • solute carrier family 40 member 1

Background

Ferroportin 1 (FPN1 or SLC40A1) is an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen etc.) that play key role in intestinal iron absorption as well as cellular iron release, and mediates iron efflux in the presence of ferroxidases hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in the iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. FPN1 can be regulated transcriptionally, post-transcriptionally, through mRNA stability and post-translationally through protein turnover and its regulation is critical for iron homeostasis because alterations in the same may lead to either iron deficiency or iron overload. FPN1 transports iron in ferrous form whereas plasma transferrin only binds iron's ferric form, and ferroxidases are key players in oxidizing iron transported by FPN1, and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types in the body utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1. Defects in FPN1 have been linked to hemochromatosis type 4 (HFE4), an autosomal dominant iron-loading disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IHC
(1)
IHC-P
(2)
WB
(26)
All Applications
Filter By Species
Human
(12)
Mouse
(17)
Rat
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NBP1-21502 Applications Species
Ashok A, Karmakar S, Chandel R et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission Exp. Eye Res. 43251 [PMID: 29859760] (ICC/IF, Human) ICC/IF Human
Lim D, Kim KS, Jeong JH et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages Nat Commun May 29 2018 [PMID: 29844422] (WB, Mouse) WB Mouse
Broide E, Reifen R, Matalon S et al. Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia. J Diabetes Res Mar 6 2018 [PMID: 29693022] (WB, Human) WB Human
Puy V, Darwiche W, Trudel S et al. Predominant role of microglia in brain iron retention in Sanfilippo syndrome, a pediatric neurodegenerative disease. Glia Apr 6 2018 [PMID: 29624734] (WB, Mouse) WB Mouse
McCarthy RC, SoSa JC, Gardeck AM et al. Inflammation-induced Iron Transport and Metabolism by Brain Microglia. J. Biol. Chem. Apr 2 2018 [PMID: 29610275] (Mouse) Mouse
Zhabyeyev P, Das SK, Basu R et al. TIMP3 deficiency exacerbates iron-overload mediated cardiomyopathy and liver disease Am. J. Physiol. Heart Circ. Physiol. 2018 Jan 26 [PMID: 29373036] (WB, Mouse) WB Mouse
Raggi C, Gammella E, Correnti M et al. Dysregulation of Iron Metabolism in Cholangiocarcinoma Stem-like Cells. Sci Rep 2017 Dec 15 [PMID: 29247214] (WB, Human) WB Human
Baba Y, Higa JK, Shimada BK et al. Protective Effects of the Mechanistic Target of Rapamycin against Excess Iron and Ferroptosis in Cardiomyocytes Am. J. Physiol. Heart Circ. Physiol. 2017 Nov 10 [PMID: 29127238] (Mouse) Mouse
Neves JV, Ramos MF, Moreira AC et al. Hamp1 but not Hamp2 regulates ferroportin in fish with two functionally distinct hepcidin types Sci Rep. 2017 Nov 01 [PMID: 29093559] (Mouse) Mouse
Zhang W, Lv T, Huang J, Ou X. Type 4B hereditary hemochromatosis associated with a novel mutation in the SLC40A1 gene: A case report and a review of the literature Medicine (Baltimore) 2017 Sep 01 [PMID: 28930842] (IHC, Human) IHC Human
Show All 36 Publications.

Reviews for Ferroportin/SLC40A1 Antibody (NBP1-21502) (7) 4.77

Average Rating: 4.7
(Based on 7 reviews)
We have 7 reviews tested in 5 species: Human, Mouse, Bovine, Pig, bovine.

Reviews using NBP1-21502:
Filter by Applications
WB
(4)
IHC-P
(2)
IHC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Bovine
(1)
Pig
(1)
bovine
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Anonymous
WB Mouse 08/08/2018
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Summary

ApplicationWestern Blot
Sample TestedAdult pancreas
SpeciesMouse
LotF-1
Western Blot Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Anonymous
WB Pig 07/12/2018
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Summary

ApplicationWestern Blot
Sample Testedduodenal mucosa
SpeciesPig
LotF-1
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Anonymous
IHC-P Human 04/04/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult eye and muscle tissue (labeled E and M, respectively)
SpeciesHuman
LotE-2

Comments

Comments1:100
Western Blot Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Anonymous
WB bovine 04/04/2018
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Summary

ApplicationWestern Blot
Sample TestedAdult eye
Speciesbovine
LotE-2

Comments

Comments1:1000
Immunohistochemistry-Paraffin Ferroportin/SLC40A1 NBP1-21502
Enlarge
reviewed by:
Anonymous
IHC-P Bovine 10/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedeye
SpeciesBovine
LotE-2

Comments

Commentsuse at 1:100
Immunohistochemistry Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Anonymous
IHC Mouse 06/27/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Western Blot Ferroportin/SLC40A1 NBP1-21502
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reviewed by:
Ajay Ashok
WB Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502). (Showing 1 - 3 of 3 FAQs).

  1. I have some leftovers of this antibody from two years ago and tried it for immunofluorescence on mouse, which did work well. I may need more of this antibody. Some things surprise me. In the old datasheet it says that there is cross reactivity with mouse. It does not say so in the new data sheet here on the web site. However, it does say so for all the conjugated versions of the Ab. What is correct? Also, in the old data sheet it says that it is for Western blotting. Now immunofluorescence is also mentioned. Is this still the same antibody? I don't have a lot number, but we got it in September 2010.
    • NBP1-21502 is still the same antibody it was two years ago. Mouse was mistakenly removed during a website update and has since been re-added. This antibody still cross-reacts with mouse. In the past two years we have begun to validate more of our products for use in ICC and this is one that has had its uses updated since you last purchased.
  2. Would it be possible to conjugate this antibody to PE or PerCP?
    • With regards to conjugating NBP1-21502SS (0.025 ml) to PE or PerCP, there are two possibilities: The first is that you could conjugate the antibody yourself, using easy-to-use kits. You would choose from Lightning-Link R-PE 3 x 0.01 mg, or Lightning-Link PerCP 3 x 0.01 mg. Because the buffer of the NBP1-21502 antibody is Tris-glycine, which is not compatible with the Lightning-Link conjugation chemistry as that targets amine groups, you would perform a buffer exchange first using the Antibody Concentration and Clean Up Kit. The lab has informed me that yes, they could conjugate your choice of PE or PerCP to this antibody for you. However, it would have to be the full-size antibody vial (0.1 ml) rather than the sample size (0.025 ml).
  3. Is the epitope for NBP1-21502 extracellular or will it require permeabilization? Is it possible to get a sample size of one of the conjugated versions?
    • The immunogen used for our Ferroportin antibody NBP1-21502 falls within a helical domain of the protein and some permeabilization is recommended for staining. Our lab uses 0.2% Tween-20 included in the blocking and diluent buffers to perform this permeabilization. As our directly conjugated antibodies are prepared per order, we unfortunately do not have sample sizes available.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-21502AF405
Alexa Fluor 488 Labeled NBP1-21502AF488
Alexa Fluor 647 Labeled NBP1-21502AF647
Alexa Fluor 700 Labeled NBP1-21502AF700
Biotin Labeled NBP1-21502B
DyLight 405 Labeled NBP1-21502V
DyLight 488 Labeled NBP1-21502G
DyLight 550 Labeled NBP1-21502R
DyLight 650 Labeled NBP1-21502C
FITC Labeled NBP1-21502F
HRP Labeled NBP1-21502H
PE Labeled NBP1-21502PE

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Recent Reviews

4.7
5
6
4
0
3
1
2
0
1
0

Anonymous
08/08/2018
Application: WB
Species: Mouse

Anonymous
07/12/2018
Application: WB
Species: Pig

Anonymous
04/04/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SLC40A1
Entrez
Uniprot