Ferritin Light Chain Antibody (8E1E7) - BSA Free

Western Blot: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Analysis using FTL mouse mAb against K562 cell lysate.

Immunocytochemistry/Immunofluorescence: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Analysis of A431 cells. Blue: DRAQ fluorescent DNA dye. Red: Actin filaments (Alexa Fluor 555 phalloidin).

Immunohistochemistry: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Immunohistochemical analysis of paraffin-embedded esophageal tissues using FTL mouse mAb with DAB staining.

Western Blot: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Western blot analysis using FTL mAb against human FTL (AA: FULL(1-157)) recombinant protein. (Expected MW is 45.5 kDa)

Western Blot: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Western blot analysis using FTL mAb against HEK293 (1) and FTL (AA: FULL(1-157))-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FTL mouse mAb with DAB staining.

ELISA: Ferritin Light Chain Antibody (8E1E7) [NBP2-37243] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 8E1E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ferritin Light Chain Antibody (8E1E7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Ferritin Light Chain (AA: FULL(1-157)) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Ferritin Light Chain Antibody (8E1E7) - BSA Free

• Ferritin L subunit
• ferritin L-chain
• ferritin light chain
• ferritin light polypeptide-like 3
• ferritin, light polypeptide
• MGC71996
• NBIA3

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FTL