Biological Strategies: Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN ...read more
Biological Strategies: Western Blot: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. Affinity purified rabbit antiFen1 antibody used for WB at 0.1 ug/ml.
Western Blot: FEN-1 Antibody [NB100-320] - Analysis of human Fen1, using NB100-320. Sample: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel.
Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit ...read more
43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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