FEN-1 Antibody

Images

 
Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Western Blot: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. Affinity purified rabbit antiFen1 antibody used for WB at 0.1 ug/ml.
Western Blot: FEN-1 Antibody [NB100-320] - Analysis of human Fen1, using NB100-320. Sample: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel.
Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

FEN-1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FEN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:30000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FEN-1 Lysate (NBL1-10676)
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-320 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FEN-1 Antibody

  • EC 3.1
  • FEN-1
  • FEN-1hFEN-1
  • flap structure-specific endonuclease 1DNase IV
  • Maturation factor 1
  • maturation factor-1
  • MF1flap endonuclease 1
  • RAD2

Background

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FEN-1 Antibody (NB100-320)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
Human
(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FEN1
Entrez
Uniprot