Western Blot: FEN-1 Antibody [NB100-320] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody used for WB at 0.1 ...read more
Western Blot: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. Affinity purified rabbit antiFen1 antibody used for WB at 0.1 ug/ml.
Western Blot: FEN-1 Antibody [NB100-320] - Analysis of human Fen1, using NB100-320. Sample: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel.
Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit ...read more
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset,Crab-eating macaque, Naked mole rat and Northern white-cheeked gibbon.
Alternate Names for FEN-1 Antibody
flap structure-specific endonuclease 1DNase IV
Maturation factor 1
MF1flap endonuclease 1
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.