FEN-1 Overexpression Lysate (Native)


Western Blot: FEN1 Overexpression Lysate (Adult Normal) [NBL1-10676] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FEN1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

FEN-1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201785 and based on accession number NM_004111. The protein contains a C-terminal DDK tag.
Homo sapiens flap structure-specific endonuclease 1 (FEN1), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for FEN-1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FEN-1 Overexpression Lysate (Native)

  • EC 3.1
  • FEN-1hFEN-1
  • flap structure-specific endonuclease 1DNase IV
  • Maturation factor 1
  • maturation factor-1
  • MF1flap endonuclease 1
  • RAD2


The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, IHC-FrFl
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu
Applications: WB, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Ba, Ch, Fi, Ha, Rb
Applications: WB, ICC/IF, IP
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ChIP, ICC/IF, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow
Species: Hu, Mu
Applications: WB, IP, PLA
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Applications: WB

Publications for FEN-1 Lysate (NBL1-10676) (0)

There are no publications for FEN-1 Lysate (NBL1-10676).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FEN-1 Lysate (NBL1-10676) (0)

There are no reviews for FEN-1 Lysate (NBL1-10676). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FEN-1 Lysate (NBL1-10676) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FEN-1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for FEN-1 Lysate (NBL1-10676)

Discover related pathways, diseases and genes to FEN-1 Lysate (NBL1-10676). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FEN-1 Lysate (NBL1-10676)

Discover more about diseases related to FEN-1 Lysate (NBL1-10676).

Pathways for FEN-1 Lysate (NBL1-10676)

View related products by pathway.

PTMs for FEN-1 Lysate (NBL1-10676)

Learn more about PTMs related to FEN-1 Lysate (NBL1-10676).

Research Areas for FEN-1 Lysate (NBL1-10676)

Find related products by research area.

Blogs on FEN-1

There are no specific blogs for FEN-1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol FEN1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-10676 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought