FEN-1 Antibody (4E7) - Azide and BSA Free

Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IP, ChIP
• Clone: 4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FEN-1 Antibody (4E7) - Azide and BSA Free Summary

• Immunogen: Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
• Localization: Nuclear
• Specificity: This is specific for FEN-1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FEN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:500-1:3000
• Application Notes: Chip, ICC/IF,IP- Assay dependent
  See Simple Western Antibody Database for Simple Western validation: separated by Size
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FEN-1 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for FEN-1 Antibody (4E7) - Azide and BSA Free

• EC 3.1
• FEN1
• FEN-1
• FEN-1hFEN-1
• flap structure-specific endonuclease 1DNase IV
• Maturation factor 1
• maturation factor-1
• MF1flap endonuclease 1
• RAD2

Background

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FEN-1 Antibody (NB100-150)(22)

Publications using NB100-150

• Uttkarsh Ayyangar, Aneesh Karkhanis, Heather Tay, Aliya Farissa Binte Afandi, Oindrila Bhattacharjee, Lalitha KS, Sze Han Lee, James Chan, Srikala Raghavan Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin The EMBO Journal 2024-02-28 [PMID: 38418556]
• Yuan B, Kikuchi H, Li J et al. Cytotoxic Effects of Darinaparsin, a Novel Organic Arsenical, against Human Leukemia Cells International Journal of Molecular Sciences 2023-01-23 [PMID: 36768603]
• Zhang J, Chen M, Pang Y et al. Flap endonuclease 1 and DNA-PKcs synergistically participate in stabilizing replication fork to encounter replication stress in glioma cells Journal of experimental & clinical cancer research : CR 2022-04-12 [PMID: 35414100] (WB, Human)
• M Kusi, M Zand, LL Lin, M Chen, A Lopez, CL Lin, CM Wang, ND Lucio, NB Kirma, J Ruan, TH Huang, K Mitsuya 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity Cell Reports, 2022-01-11;38(2):110220. 2022-01-11 [PMID: 35021081]
• Zhang J, Jing L, Tan S et al. Inhibition of miR-1193 leads to synthetic lethality in glioblastoma multiforme cells deficient of DNA-PKcs Cell Death Dis 2020-07-30 [PMID: 32732911]
• Jaiswal AS, Armas ML, Izumi T et al. Adenomatous Polyposis Coli Interacts with Flap Endonuclease 1 to Block Its Nuclear Entry and Function Neoplasia 2012-06-01 [PMID: 22787431] (WB, Human)
• Ma Y, Liang D, Liu J et al. Prostate Cancer Cell Lines under Hypoxia Exhibit Greater Stem-Like Properties. PLoS ONE 6(12). 2011-01-01 [PMID: 22216200]
• Tann AW, Boldogh I, Meiss G et al. Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair. J Biol Chem 2011-09-01 [PMID: 21768646] (Human)
• Jaiswal AS, Narayan S. Assembly of the Base Excision Repair Complex on Abasic DNA and Role of Adenomatous Polyposis Coli on Its Functional Activity. Biochemistry;50(11):1901-9. 2011-03-22 [PMID: 21261287]
• Guo Zhigang, Zheng Li, Xu Hong et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol. 2010-10-01 [PMID: 20729856] (Human)
• Asagoshi K, Liu Y, Masaoka A et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst) 2010-02-01 [PMID: 20006562] (Human)
• Kong X, Mohanty SK, Stephens J et al. Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells. Nucleic Acids Res 2009-05-01 [PMID: 19357094] (Human)
• Guo Z, Zheng L, Dai H et al. Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity. Nucleic Acids Res 2009-06-01 [PMID: 19336415] (Human)
• Szczesny B, Tann AW, Longley MJ et al. Long patch base excision repair in mammalian mitochondrial genomes. J Biol Chem 2008-09-01 [PMID: 18635552] (Human)
• Liu P, Qian L, Sung JS et al. Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria. Mol Cell Biol 2008-08-01 [PMID: 18541666] (Human)
• Guo Z, Qian L, Liu R et al. Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair. Mol Cell Biol 2008-07-01 [PMID: 18443037] (Human)
• Guo Z, Chavez V, Singh P et al. Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways. J Mol Biol 2008-03-01 [PMID: 18291413] (Human)
• Prasad R, Liu Y, Deterding LJ et al. HMGB1 is a cofactor in mammalian base excision repair. Mol Cell 2007-09-01 [PMID: 17803946] (Human)
• Kundu, C et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis;28: 2089-2095. [PMID: 17522063]
• Kedar PS, Kim SJ, Robertson A et al. Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen. J Biol Chem 2002-08-01 [PMID: 12063248] (Human)
• Shibata Y, Nakamura T. Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay. J Biol Chem 2002-01-01 [PMID: 11687589] (Human)
• Qiu, J et al. Cell Cycle-dependent DNA Damage-inducible Nuclear Localization of FEN-1 Nuclease Is Consistent with Its Dual Functions in DNA Replication Repair. The Journal of Biological Chemistry 276(7): 4901-4908. 2001-01-01 [PMID: 11053418]

Bioinformatics

• Gene Symbol: FEN1
• Entrez:
  • 2237(Human)
• Uniprot:
  • P39748(Human)