FEM1B Antibody

Images

 
Western Blot: FEM1B Antibody [NBP1-77300] - Analysis of FEM1B in mouse (A) and rat (B) liver tissue lysates with anti- FEM1B using this antibody at 1 ug /ml.
Immunocytochemistry/ Immunofluorescence: FEM1B Antibody [NBP1-77300] - Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: FEM1B Antibody [NBP1-77300] - mouse liver tissue with FEM1B antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: FEM1B Antibody [NBP1-77300] - Staining of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FEM1B Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A. The immunogen is located within the last 50 amino acids of F1A alpha.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FEM1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
FEM1B antibody can be used for detection of F1Ab by Western blot at 0.5 to 1 mg/ml. An approximately 70 kDa band can be detected. It is human, mouse, and rat reactive.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
FEM1B Peptide (NBP1-77300PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Chicken: (79%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FEM1B Antibody

  • DKFZp451E0710
  • F1AA
  • F1A-alpha
  • FEM-1 (C.elegans) homolog b
  • fem-1 homolog b (C. elegans)
  • FEM1b
  • FEM1-beta
  • FEM-1-like death receptor binding protein
  • Fem-1-like death receptor-binding protein alpha
  • Fem-1-like in apoptotic pathway protein alpha
  • FIAA
  • KIAA0396
  • protein fem-1 homolog B

Background

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1beta. F1Aalpha/FEM1beta is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FEM1B
Uniprot