FEM1C Antibody

Western Blot: FEM1C Antibody [NB100-784] - Analysis of FEM1C in Hela lysate using NB100-784 at 2 ug/ ml.

Western Blot: FEM1C Antibody [NB100-784] - (0.5ug/ml) staining of rat kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FEM1C Antibody Summary

• Description: Novus Biologicals Goat FEM1C Antibody (NB100-784) is a polyclonal antibody validated for use in WB and ELISA. Anti-FEM1C Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HIPEKLETFVSLHR corresponding to C-Terminus according to NP_064562.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FEM1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in heart and kidney lysates from mouse and rat (calculated MW of 68.6 kDa band according to mouse NP_775599.1).
• Control:

  FEM1C Overexpression Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FEM1C Antibody

• EUROIMAGE686608
• EUROIMAGE783647
• fem-1 homolog c (C. elegans)
• FEM1A
• FEM1c
• KIAA1785FEM1-gamma
• protein fem-1 homolog C

Background

A caspase substrate homologous to the Caenorhabditis elegans sex-determining protein, FEM-1.Principle Names - FEM1A; feminization 1 homolog a; KIAA1785; EUROIMAGE686608; EUROIMAGE783647

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FEM1C Antibody (NB100-784)(7)

Publications using NB100-784

• Pritchard KA, Jing X, Teng M et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia PloS one 2022-08-26 [PMID: 36018859]
• Clift D, McEwan WA, Labzin LI et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017-11-13 [PMID: 29153837]
• Teng RJ, Jing X, Michalkiewicz T et al. Attenuation of endoplasmic reticulum stress by caffeine ameliorates hyperoxia-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-05-01 [PMID: 28213471]
• de la Pisa IG, Cebrian C, Ortega JE et al. Cytokine pathway disruption in a mouse model of schizophrenia induced by Munc18-1a overexpression in the brain. J Neuroinflammation 2014-07-29 [PMID: 25069615]
  
  Details:
  CD11b antibody used for WB application at 1:1000 dilution in mouse model of schizophrenia induced by Munc18-1a overexpression/OE in the brain (see full text for sample preparation details). Figures 3a and 4a shows the WB of microglia /macrophage marker CD11bas single band of 160 kDa in cerebral cortex and striatum tissues of Munc18-OE and wild-type mice.
• Roehlecke C, Schumann U, Ader M et al. Stress Reaction in Outer Segments of Photoreceptors after Blue Light Irradiation. PLoS One. 2013-09-11 [PMID: 24039718]
• Koen YM, Sarma D, Hajovsky H et al. Protein Targets of Thioacetamide Metabolites in Rat Hepatocytes Chem Res Toxicol 2013-03-20 [PMID: 23465048]
• Chan SL, Tan KO, Zhang L et al. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem 1999-11-05 [PMID: 10542291]

Bioinformatics

• Gene Symbol: FEM1C
• Entrez:
  • 56929(Human)
  • 240263(Mouse)
  • 302288(Rat)
• Uniprot:
  • NP_064562.(Human)