Fc gamma RIII (CD16) Antibody (1001049) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
1001049
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Fc gamma RIII (CD16) Antibody (1001049) [Alexa Fluor® 647] Summary

Immunogen
Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a
Gly17-Gln208
Accession # P08637
Specificity
Detects human Fc gamma RIIIA/CD16a in direct ELISAs. In direct ELISAs, 60% cross-reactivity with Fc gamma RIIIB/CD16b is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody (1001049) [Alexa Fluor® 647]

  • CD16
  • CD16A
  • Fc fragment of IgG receptor IIIa
  • Fc gamma RIII
  • FCG3
  • FCGR3
  • FcgRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3
  • IMD20

Background

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

  1. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19. 
  2. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481. 
  3. Wu, J. et al. (1997) J. Clin. Invest. 100:1059. 
  4. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664. 
  5. Kim, M.-K. et al. (2003) Blood 101:4479. 
  6. Lanier, L.L. et al. (1989) Nature 342:803.
  7. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
  8. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  9. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  10. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  11. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4325U
Alexa Fluor 405 FAB4325V
Alexa Fluor 488 FAB4325G
Alexa Fluor 594 FAB4325T
Alexa Fluor 647 FAB4325R
Alexa Fluor 700 FAB4325N
Alexa Fluor 750 FAB4325S

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