Fc gamma RIIB/C (CD32b/c) Antibody (190710)

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 190710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Fc gamma RIIB/C (CD32b/c) Antibody (190710) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
  Ala46-Pro217
  Accession # P31994
• Specificity: Detects human Fc gamma RIIB/C in ELISAs. In a sandwich ELISA shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human Fc gamma RIIB/C (CD32b/c) Antibody (Catalog # MAB18751)
  ELISA Detection: Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody (Catalog # BAM1875)
  Standard: Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIIB/C (CD32b/c) Antibody (190710)

• CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b
• Fc gamma RIIB/C (CD32b/c)

Background

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains with Fc gamma RIIB and Fc gamma RIIC having identical extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RIIB/C (CD32b/c) Antibody (MAB18751)(2)

Publications using MAB18751

• C Ling, MD Cook, H Grimm, M Aldokhayyi, D Gomez, M Brown The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells Mediators of Inflammation, 2021-12-02;2021(0):6687250. 2021-12-02 [PMID: 34899053] (Neutralization, Human)
• Page G, Miossec P Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression. J. Pathol., 2004-09-01;204(1):28-38. 2004-09-01 [PMID: 15307135] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FCGR2B
• Uniprot:
  • P31994()