Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Mu
• Applications: WB, Flow, Func, ICC/IF, IHC, B/N
• Clone: AT130-5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: CD32-rat CD4 domain 3/4 fusion protein.
• Specificity: This antibody is specific for murine Fc gamma RII/CD32, which is a Fc gamma receptor that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. Fc gamma RII/CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCGR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  
  The antibody binds to CD32, a surface receptor protein with a low affinity for IgG antibodies.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B