Fc gamma RI/CD64 Antibody (290322)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
290322
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Fc gamma RI/CD64 Antibody (290322) Summary

Immunogen
NS0-derived recombinant mouse Fc gamma RI extracellular domain
Glu25-Pro297
Accession # P26151
Specificity
Detects mouse Fc gamma  RI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fc gamma  RIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Fcgr1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
MAB20741 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RI/CD64 Antibody (290322)

  • CD64 antigen
  • CD64
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • Fc gamma RI
  • FCG1
  • Fc-gamma receptor I B2
  • Fc-gamma RI
  • Fc-gamma RIA
  • FcgammaRIa
  • FCGR1
  • FcgRI
  • FCRI
  • FLJ18345
  • high affinity Ia, receptor for (CD64)
  • high affinity immunoglobulin gamma Fc receptor I
  • IgG Fc receptor I

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8‑10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6‑10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain (1, 2). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RI/CD64 Antibody (MAB20741)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: Flow, ICC, IHC OCT-embedded, In vivo.


Filter By Application
Flow
(5)
ICC
(1)
IHC OCT-embedded
(1)
In vivo
(1)
All Applications
Filter By Species
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB20741 Applications Species
SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29277896] (Flow,, Mouse) Flow Mouse
P Cunin, LR Penke, JN Thon, PA Monach, T Jones, MH Chang, MM Chen, I Melki, S Lacroix, Y Iwakura, J Ware, MF Gurish, JE Italiano, E Boilard, PA Nigrovic Megakaryocytes compensate for Kit insufficiency in murine arthritis J. Clin. Invest., 2017;127(5):1714-1724. 2017 [PMID: 28375155] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
D Schlam, J Canton, M Carreño, H Kopinski, SA Freeman, S Grinstein, GD Fairn Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis MBio, 2016;7(2):. 2016 [PMID: 27048806] (Flow,, Mouse) Flow Mouse
Negishi-Koga T, Gober H, Sumiya E, Komatsu N, Okamoto K, Sawa S, Suematsu A, Suda T, Sato K, Takai T, Takayanagi H Immune complexes regulate bone metabolism through FcRgamma signalling. Nat Commun, 2015;6(0):6637. 2015 [PMID: 25824719] (Flow,, Mouse) Flow Mouse
Qu L, Zhang P, Lamotte RH, Ma C Neuronal Fc-gamma receptor I mediated excitatory effects of IgG immune complex on rat dorsal root ganglion neurons. Brain Behav. Immun., 2011;25(0):1399-407. 2011 [PMID: 21521651] (ICC,, Rat) ICC Rat
Jonsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daeron M, Bruhns P Mouse and human neutrophils induce anaphylaxis. J. Clin. Invest., 2011;121(4):1484-96. 2011 [PMID: 21436586] (In vivo,, Mouse) In vivo Mouse
Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets. J. Cell Biol., 2010;191(6):1205-18. 2010 [PMID: 21135140] (Flow,, Mouse) Flow Mouse
Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Este JA beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication. J. Immunol., 2011;186(1):464-70. 2011 [PMID: 21098231] (Flow,, Mouse) Flow Mouse

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Bioinformatics

Gene Symbol Fcgr1
Entrez
Uniprot