Fc gamma RI/CD64 Antibody (290322) [PerCP (Peridinin-chlorophyll Protein Complex)]

• Applications: Flow
• Clone: 290322
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

Fc gamma RI/CD64 Antibody (290322) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: NS0-derived recombinant mouse Fc gamma RI extracellular domain
  Glu25-Pro297
  Accession # P26151
• Specificity: Detects mouse Fc gamma RI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fc gamma RIA, rhFc gamma RIIB, or rhFc gamma RIIIB is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FCGR1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10^‑8‑10^‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^‑6‑10^‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain (1, 2). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR1A