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FBRS Antibody (NBP1-83917) (1)
Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.
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Reviews using NBP1-83917:
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Application Western Blot Sample Tested Hela whole cell lysate Species Human Lot R40759 Blocking
Blocking Details Blocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT Primary Anitbody
Dilution Ratio 1:500 over night at 4 degree Celsius Secondary Antibody
Secondary Manufacturer Cat# 7074P2 Secondary Concentration 1:20,000 Details
Detection Notes 90 seconds exposure Comments
Comments I would suggest to keep the blotted-membrane exposed to the x-ray film for longer. The FBRS protien band appears but with a major band of ~90 kDa. However, worked for me at least.
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