Fascin Antibody (SAIC-32C-205) - Azide and BSA Free

Flow Cytometry: Fascin Antibody (SAIC-32C-205) - Azide and BSA Free [NBP3-20100] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-32C-205 (NBP3-20100, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry/Immunofluorescence: Fascin Antibody (SAIC-32C-205) - Azide and BSA Free [NBP3-20100] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-32C-205 (NBP3-20100) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20100, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF, MS
• Clone: SAIC-32C-205
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Fascin Antibody (SAIC-32C-205) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Fascin Antibody (SAIC-32C-205) - Azide and BSA Free (NBP3-20100) is a recombinant monoclonal antibody validated for use in Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide "TVEEAENIAVTSGVVR" derived from Fascin, which organises filamentous actin into bundles, conjugated to KLH.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FSCN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Mass Spectrometry
• Application Notes: This Ab can be used in immunoMRM.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fascin Antibody (SAIC-32C-205) - Azide and BSA Free

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1