Fascin Antibody (833223) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clone: 833223
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Fascin Antibody (833223) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Fascin
  Met1-Tyr493
  Accession # Q16658
• Specificity: Detects human Fascin in ELISAs and Western blots. Detects mouse and rat Fascin in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fascin-2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fascin Antibody (833223) [Alexa Fluor® 700]

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Fascin (that which creates fascicles [bundles] of actin; also known as 55 kDa actin-bundling protein, p55 and singed-like protein) is an intracellular 55-58 kDa member of the fascin family of proteins. It has a restricted expression pattern, being found in oligodendrocytes, select endothelium, cerebellar stellate neurons and blood, interdigitating, and thymic medullary dendritic cells. Fascin is found associated with actin in filopodia, and serves to coordinate and stabilize actin bundle formation, both in normal cells and tumor cells. In the latter cell type, filopodia have been renamed invadopodia, and their appearance is crucial for the creation of a stable platform that coordinates local matrix degradation. Human fascin is 493 amino acids (aa) in length. It contains an N-terminal fascin-like domain (aa 139-256) that contains part of one of two actin-binding sequences (aa 136-143), followed by two additional fascin-like domains (aa 260-378 and 383-493), the latter of which contains the second actin-binding sequence (aa 386-395). There are also two acetylation sites and two utilized phosphorylation sites at Ser38 and Ser39. Phosphorylation of the latter site inhibits fascin interaction with actin. At least two isoform variants may exist. One contains a 12 aa substitution for aa 427-493, while a second shows a deletion of aa 371-426. Full-length human fascin shares 97% aa sequence identity with mouse fascin. Two additional human fascins termed retinal and testis fascin have been identified. They are products of distinct genes and share 56% and 27% aa sequence identity with the standard (p55) fascin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.