FANCD2 Antibody (FI-17)

Images

 
Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Simple Western lane view shows a specific band for FANCD2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using NB100-316.
Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - ...read more

Product Details

Summary
Reactivity Hu, Ha(-), Mu(-)Species Glossary
Applications WB, Simple Western, ChIP, IP
Clone
FI-17
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FANCD2 Antibody (FI-17) Summary

Immunogen
Human FANCD2 fusion protein [Swiss-Prot# Q9BXW9]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FANCD2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Simple Western 1:50
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation Assay dependent
Application Notes
This FANCD2 (FI-17) antibody may be used for Western blot, Chromatin Immunoprecipitation (Pubmed ID 19748364) and Immunoprecipitation. By Western blot, this antibody recognizes a band at ~155 and ~162 kDa (post-translationally modified form).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-316 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

This antibody does not react with mouse or hamster.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for FANCD2 Antibody (FI-17)

  • DKFZp762A223
  • FA4
  • FACD
  • FACDFLJ23826
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • Protein FACD2

Background

FANCD2 (Fanconi anemia group D2 protein) belongs to family of FANC proteins which includes several members such as FANC-A, -B, -C, -D1/BRCA2, -D2, -E, -F, -G, -I, -J/BRIP1, -L, -M and -N/PALB2. FANCD2 interacts with several proteins such as FANCE/FANCI, USP1, MEN1, BRCA1, BRCA2, BLM, MTMR15/FAN1 and DCLRE1B/Apollo. FANC proteins (-A, -B, -C, -D1, -D2, -E, -F, -G, -L) cooperate in FANC/BRCA pathway resulting in monoubiquitination of FANCD2 which then promotes BRCA2/FANCD1 loading onto chromatin for creating nuclear foci at DNA damage sites. It is involved in DNA double-strand breaks repair, both by homologous recombination and single-strand annealing. FANCD2 is required for maintenance of chromosomal stability and promotes accurate/efficient pairing of homologs during meiosis. FANCD2 may participate in S phase/G2 phase checkpoint activation upon DNA damage amd plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 characterized by anemia, leukopenia and thrombopenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FANCD2 Antibody (NB100-316)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-316 Applications Species
Nalepa G, Enzor R, Sun Z et al. Fanconi anemia signaling network regulates the spindle assembly checkpoint. J Clin Invest. 2013 Sep 3 [PMID: 23934222] (WB, Human) WB Human
Kusayanagi T, Tsukuda S, Shimura S et al. The antitumor agent doxorubicin binds to Fanconi anemia group F protein Bioorg Med Chem 2012 Sep 16 [PMID: 23026082] (WB, Human) WB Human
Park S, Beck B, Saadatzadeh M et al. Fanconi anemia D2 protein is an apoptotic target mediated by caspases J Cell Biochem 2011 Sep [PMID: 21520247] (WB, Human) WB Human
Bhagwat N, Olsen AL, Wang AT et al. XPF-ERCC1 participates in the Fanconi anemia pathway of crosslink repair. Mol Cell Biol :MCB.00086-00009. 2009 [PMID: 19805513]
Chu P-C, Wang T-Y, Lu Y-T et al. Involvement of p29 in DNA damage responses and Fanconi anemia pathway. Carcinogenesis;30(10):1710-1716. 2009 [PMID: 19748926]
Willers H, Taghian AG, Luo C-M et al. Utility of DNA Repair Protein Foci for the Detection of Putative BRCA1 Pathway Defects in Breast Cancer Biopsies. Mol Cancer Res;7(8):1304-1309. 2009 [PMID: 19671671]
Si, Y et al. Overnight transduction with foamyviral vectors restores the long-term repopulating activity of Fancc-/- stem cells. Blood;112(12):4458-65. 2008 Dec 1. [PMID: 18684868]
Tremblay, CS et al. HES1 is a novel interactor of the Fanconi anemia core complex. Blood;112(5):2062-70. 2008 Sep 1. [PMID: 18550849]
Nomura, Y et al. Human Mus81 and FANCB independently contribute to repair of DNA damage during replication. Genes Cells;12(10):1111-22. 2007 Oct. [PMID: 17903171]
Park, S et al. Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins. J Biol Chem;279(29):30053-9. 2004 Jul 16. [PMID: 15138265] (WB, IP, Human) WB, IP Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for FANCD2 Antibody (NB100-316). (Showing 1 - 2 of 2 FAQs).

  1. Can you provide the aa sequence used to generate this antibody? Is it the full length protein?
  2. Do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?
    • Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FANCD2 Products

FANCD2 NB100-316

Bioinformatics Tool for FANCD2 Antibody (NB100-316)

Discover related pathways, diseases and genes to FANCD2 Antibody (NB100-316). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FANCD2 Antibody (NB100-316)

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Pathways for FANCD2 Antibody (NB100-316)

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PTMs for FANCD2 Antibody (NB100-316)

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Research Areas for FANCD2 Antibody (NB100-316)

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Blogs on FANCD2.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

FANCD2: DNA Repair and Beyond
Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Bioinformatics

Gene Symbol FANCD2
Entrez
Uniprot