Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Simple Western lane view shows a specific band for FANCD2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using NB100-316.
Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - ...read more
This FANCD2 (FI-17) antibody may be used for Western blot, Chromatin Immunoprecipitation (Pubmed ID 19748364) and Immunoprecipitation. By Western blot, this antibody recognizes a band at ~155 and ~162 kDa (post-translationally modified form).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
This antibody does not react with mouse or hamster.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Alternate Names for FANCD2 Antibody (FI-17)
Fanconi anemia group D2 protein
Fanconi anemia, complementation group D2
FANCD2 (Fanconi anemia group D2 protein) belongs to family of FANC proteins which includes several members such as FANC-A, -B, -C, -D1/BRCA2, -D2, -E, -F, -G, -I, -J/BRIP1, -L, -M and -N/PALB2. FANCD2 interacts with several proteins such as FANCE/FANCI, USP1, MEN1, BRCA1, BRCA2, BLM, MTMR15/FAN1 and DCLRE1B/Apollo. FANC proteins (-A, -B, -C, -D1, -D2, -E, -F, -G, -L) cooperate in FANC/BRCA pathway resulting in monoubiquitination of FANCD2 which then promotes BRCA2/FANCD1 loading onto chromatin for creating nuclear foci at DNA damage sites. It is involved in DNA double-strand breaks repair, both by homologous recombination and single-strand annealing. FANCD2 is required for maintenance of chromosomal stability and promotes accurate/efficient pairing of homologs during meiosis. FANCD2 may participate in S phase/G2 phase checkpoint activation upon DNA damage amd plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 characterized by anemia, leukopenia and thrombopenia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FANCD2 and DNA damage repair Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t... Read full blog post.
FANCD2 (Fanconi anemia subunit D2 protein) Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama... Read full blog post.
FANCD2: DNA Repair and Beyond Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch... Read full blog post.
NUP153 & 53BP1: A Novel DNA Repair Pathway Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De... Read full blog post.
Fanconi Antibodies and Cancer Research We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)... Read full blog post.
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