FANCC Antibody - Azide and BSA Free

Western Blot: FANCC Antibody [NB100-2565] - Detection of FANC C in transfected COS1 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FANCC Antibody - Azide and BSA Free Summary

• Immunogen: A C-terminal peptide within residues 450-558 of human FANCC. [SwissProt# Q00597]
• Localization: The major form is nuclear. The minor form is cytoplasmic.
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This FANCC antibody is useful for Western Blot where a band is seen at approximately 65 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FANCC Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (92%), mouse (92%) and bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for FANCC Antibody - Azide and BSA Free

• FA Complementation Group C
• FA3
• FACC
• FACCProtein FACC
• FACFanconi anemia group C protein
• FANCC
• Fanconi anemia, complementation group C
• FLJ14675

Background

FANCC (Fanconi anemia group C protein) is a DNA repair protein which implicates in post-replication DNA repair or a cell cycle checkpoint function. FANCC also involves in the interstrand DNA cross-link repair as well as the maintenance of chromosome stability/integrity. FANCC participates in the multisubunit FA complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM and HSP70) which is not found in FA patients. Upon IFNG induction, FANCC facilitate STAT1 activation via recruitement of STAT1 to IFNGR1. FANCC interacts with ZBTB32 (upon IFNG induction), CDK1, STAT1 etc and FANCC-EIF2AK2 interactions leads to augmented EIF2AK2 activation and cell death. FANCC is expressed ubiquitously and the major form is nuclear while the minor form is cytoplasmic. Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC), disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCC Antibody (NB100-2565)(1)

Publication using NB100-2565

• Yung SK, Tilgner K, Ledran MH et al. Human pluripotent stem cell models of Fanconi Anaemia deficiency reveal an important role for Fanconi Anaemia proteins in cellular reprogramming and survival of haematopoietic progenitors. Stem Cells 2012-12-27 [PMID: 23280624] (WB)

Bioinformatics

• Gene Symbol: FANCC
• Entrez:
  • 2176(Human)
  • 14088(Mouse)
  • 24361(Rat)
• Uniprot:
  • Q00597(Human)