FACL4 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

FACL4 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human FACL4.

  Source: E. coli

  Amino Acid Sequence: EAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCER

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: ACSL4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-55119.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for FACL4 Recombinant Protein Antigen

• ACS4mental retardation, X-linked 68
• acyl-CoA synthetase 4
• acyl-CoA synthetase long-chain family member 4
• EC 6.2.1.3
• FACL4long-chain 4
• LACS 4
• LACS4MRX68
• lignoceroyl-CoA synthase
• Long-chain acyl-CoA synthetase 4
• long-chain fatty-acid-Coenzyme A ligase 4
• long-chain-fatty-acid--CoA ligase 4
• mental retardation, X-linked 63
• MRX63

Background

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ACSL4