FACL4 Overexpression Lysate (Native)

Images

 
Western Blot: FACL4 Overexpression Lysate (Adult Normal) [NBL1-07264] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FACL4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FACL4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205356 and based on accession number NM_004458. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 1, mRNA.
Gene
ACSL4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
74.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FACL4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FACL4 Overexpression Lysate (Native)

  • ACS4mental retardation, X-linked 68
  • acyl-CoA synthetase 4
  • acyl-CoA synthetase long-chain family member 4
  • EC 6.2.1.3
  • FACL4long-chain 4
  • LACS 4
  • LACS4MRX68
  • lignoceroyl-CoA synthase
  • Long-chain acyl-CoA synthetase 4
  • long-chain fatty-acid-Coenzyme A ligase 4
  • long-chain-fatty-acid--CoA ligase 4
  • mental retardation, X-linked 63
  • MRX63

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the predominant isoform (1) of 670 amino acids. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FACL4 Lysate (NBL1-07264) (0)

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Bioinformatics

Gene Symbol ACSL4