FACL4 Antibody

Western Blot: FACL4 Antibody [NB300-861] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NB300-861] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: FACL4 Antibody [NB300-861] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FACL4 Antibody Summary

• Immunogen: Peptide with sequence C-HYLKDIERMYGGK corresponding to C-Terminus according to NP_075266.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACSL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 80 kDa band observed in HepG2 lysates (calculated MW of 79.2 kDa band according to NP_075266.1).
• Control:

  FACL4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FACL4 Antibody

• ACS4mental retardation, X-linked 68
• acyl-CoA synthetase 4
• acyl-CoA synthetase long-chain family member 4
• EC 6.2.1.3
• FACL4long-chain 4
• LACS 4
• LACS4MRX68
• lignoceroyl-CoA synthase
• Long-chain acyl-CoA synthetase 4
• long-chain fatty-acid-Coenzyme A ligase 4
• long-chain-fatty-acid--CoA ligase 4
• mental retardation, X-linked 63
• MRX63

Background

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FACL4 Antibody (NB300-861)(2)

Publications using NB300-861

• Cheng L, Zhu X, Liu Y et al. ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway Annals of Translational Medicine 2021-09-01 [PMID: 34734006]
• Piccini M, Vitelli F, Bruttini M et al. FACL4, a new gene encoding long-chain acyl-CoA synthetase 4, is deleted in a family with Alport syndrome, elliptocytosis, and mental retardation. Genomics 1998-02-01 [PMID: 9480748]

Bioinformatics

• Gene Symbol: ACSL4
• Entrez:
  • 2182(Human)
  • 50790(Mouse)
  • 113976(Rat)
• Uniprot:
  • NP_004449(Human)