FABP2/I-FABP Antibody

Images

 
Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: FABP2/I-FABP Antibody [NB100-59746] - HEK293 overexpressing FABP2 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, Mu, FiSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FABP2/I-FABP Antibody Summary

Immunogen
Peptide with sequence C-EGVEAKRIFKKD corresponding to C-Terminus according to NP_000125.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.001 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2-3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
Western blot: A very strong approx 16kDa band observed in Human and Mouse Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). In transfected HEK293 transiently expressing FABP2 a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Duodenum Whole Tissue Lysate (Adult Whole Normal)
FABP2/I-FABP Overexpression Lysate
Control Peptide
Publications
Read Publications using
NB100-59746 in the following applications:

  • IHC
    2 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22790967). Fish reactivity reported in multiple pieces of scientific literature. Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FABP2/I-FABP Antibody

  • FABP2
  • FABPI
  • fatty acid binding protein 2, intestinal
  • Fatty acid-binding protein 2
  • fatty acid-binding protein, intestinal
  • IFABP
  • I-FABP
  • I-FABPMGC133132
  • Intestinal FABP
  • Intestinal-type fatty acid-binding protein

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP2/I-FABP Antibody (NB100-59746)(5)

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Bioinformatics

Gene Symbol FABP2
Entrez
Uniprot