FABP2/I-FABP Antibody (9A9B7B3)

Western Blot: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.

Immunocytochemistry/Immunofluorescence: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of 3T3-L1 cells using FABP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of paraffin-embedded human Small Intestine tissues.

Flow Cytometry: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Flow cytometric analysis of LOVO cells using FABP2 mouse mAb (green) and negative control (purple).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 9A9B7B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FABP2/I-FABP Antibody (9A9B7B3) Summary

• Immunogen: Purified recombinant fragment of human FABP2/I-FABP expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FABP2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for FABP2/I-FABP Antibody (9A9B7B3)

• FABP2
• FABPI
• fatty acid binding protein 2, intestinal
• Fatty acid-binding protein 2
• fatty acid-binding protein, intestinal
• IFABP
• I-FABP
• I-FABPMGC133132
• Intestinal FABP
• Intestinal-type fatty acid-binding protein

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FABP2/I-FABP Antibody (NBP1-51589)(2)

Publications using NBP1-51589

• Wakao S, Oguma Y, Kushida Y et al. Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame Research Square 2022-03-08 [PMID: 36203068] (ICC/IF, Human)
• Workman MJ, Gleeson JP, Troisi EJ et al. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol 2017-12-29 [PMID: 29930984] (ICC/IF, Human)

FAQs for FABP2/I-FABP Antibody (NBP1-51589). (Showing 1 - 1 of 1 FAQs).

1. In your product page of FABP2/I-FABP Antibody (9A9B7B3), FABP2 is detected at the molecular size around 17kDa in LOVO cells. Overexpressed FABP2 was detected at the around 43kDa in HEK293 cells as a single band without lower size band. I wonder why over expressed FABP2 is detected at 42-43kDa without 17kDa band. Do you have any explanation for this finding?
   • The Western blot shown on our datasheet is of an analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.  Fc tag alon is 26 kDa and with the 15 kDa FABP2 sequence, Fc-fused FABP2 runs at ~41 kDa.   The protein alone should run at ~15 kDa.

Bioinformatics

• Gene Symbol: FABP2