FABP2/I-FABP Antibody (9A9B7B3)

Images

 
Western Blot: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of 3T3-L1 cells using FABP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled ...read more
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of paraffin-embedded human Small Intestine tissues.
Flow Cytometry: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Flow cytometric analysis of LOVO cells using FABP2 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
9A9B7B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FABP2/I-FABP Antibody (9A9B7B3) Summary

Immunogen
Purified recombinant fragment of human FABP2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FABP2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-51589 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for FABP2/I-FABP Antibody (9A9B7B3)

  • FABP2
  • FABPI
  • fatty acid binding protein 2, intestinal
  • Fatty acid-binding protein 2
  • fatty acid-binding protein, intestinal
  • IFABP
  • I-FABP
  • I-FABPMGC133132
  • Intestinal FABP
  • Intestinal-type fatty acid-binding protein

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP2/I-FABP Antibody (NBP1-51589)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FABP2/I-FABP Antibody (NBP1-51589). (Showing 1 - 1 of 1 FAQ).

  1. In your product page of FABP2/I-FABP Antibody (9A9B7B3), FABP2 is detected at the molecular size around 17kDa in LOVO cells. Overexpressed FABP2 was detected at the around 43kDa in HEK293 cells as a single band without lower size band. I wonder why over expressed FABP2 is detected at 42-43kDa without 17kDa band. Do you have any explanation for this finding?
    • The Western blot shown on our datasheet is of an analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.  Fc tag alon is 26 kDa and with the 15 kDa FABP2 sequence, Fc-fused FABP2 runs at ~41 kDa.   The protein alone should run at ~15 kDa.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FABP2