Apolipoprotein CIII Antibody

Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Apolipoprotein CIII Antibody Summary

• Immunogen: produced from Apolipoprotein CIIII derived from human plasma (Uniprot: P02656)
• Specificity: Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.
• Control:

  Apolipoprotein CIII Overexpression Lysate

Reactivity Notes

Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Bovine reactivity reported in scientific literature (PMID: 29852444).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Apolipoprotein CIII Antibody

• APOC3
• APOCIII
• apo-CIII
• apoC-III
• Apolipoprotein C3
• apolipoprotein C-III
• MGC150353

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein antibody is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein CIII Antibody (NB600-610)(2)

Publications using NB600-610

• Girolami F, Badino P, Spalenza V et al. Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: ultrastructural and biochemical findings Sci. Total Environ. 2018-05-28 [PMID: 29852444] (WB, Bovine)
• Wang Y, Song Z, Wagner JD et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in non-human primate serum. J Lipid Res;52(6):1265-71. 2011-06-01 [PMID: 21371998]

Review for Apolipoprotein CIII Antibody (NB600-610) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: System Admin ELISA Human 11/03/2009

Summary

• Application: ELISA
• Sample Tested: Human serum
• Species: Human
• Lot: 21761

Bioinformatics

• Gene Symbol: APOC3
• Entrez:
  • 345(Human)
• Uniprot:
  • P02656(Human)