Apolipoprotein CIII Antibody

Images

 
Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Apolipoprotein CIII Antibody Summary

Immunogen
Produced from Apolipoprotein CIII derived from human plasma. Uniprot ID P02656
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:10000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Control
Apolipoprotein CIII Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB600-610 in the following applications:

Publications
Read Publications using
NB600-610 in the following applications:

  • WB
    1 publication

Reactivity Notes

Specific cross-reaction with Apolipoprotein C-III from other species has not been determined Non-specific cross-reaction with other human serum proteins is negligible.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein CIII Antibody

  • APOC3
  • APOCIII
  • apo-CIII
  • apoC-III
  • Apolipoprotein C3
  • apolipoprotein C-III
  • MGC150353

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein antibody is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein CIII Antibody (NB600-610)(2)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Apolipoprotein CIII Antibody (NB600-610) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-610:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 11/03/2009
View

Summary

ApplicationELISA
Sample TestedHuman serum
SpeciesHuman
Lot21761
CommentsNovus antibody used as plate coating for Sandwich ELISA assay. Had problems getting useful ApoC3 standard-- abandoned assay. Currently using a new kit.

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: PBS, Primary Ab Dilution Ratio: 1/1000, Incubation Time:1hr, Incubation Temp:22C

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse Ig, Secondary Ab Dilution: 1/1000

Comments

CommentsNovus antibody used as plate coating for Sandwich ELISA assay. Had problems getting useful ApoC3 standard-- abandoned assay. Currently using a new kit.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Apolipoprotein CIII Antibody (NB600-610) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
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1
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Anonymous
11/03/2009
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol APOC3
Entrez
Uniprot