FABP1/L-FABP Antibody (965608) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29643) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Rt
• Applications: WB, Simple Western, ELISA
• Clone: 965608
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FABP1/L-FABP Antibody (965608) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FABP1/L-FABP
  Met1-Ile127
  Accession # P07148
• Specificity: Detects human and rat FABP1/L-FABP in direct ELISAs and Western blots. In sandwich immunoassays, it is specific for human FABP1/L-FABP.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP1/L-FABP Antibody (965608) [Unconjugated]

• FABP1
• FABPL
• fatty acid binding protein 1, liver
• fatty acid-binding protein, liver
• LFABP
• L-FABP
• L-FABPFatty acid-binding protein 1
• Liver-type fatty acid-binding protein

Background

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P07148()