Cannabinoid R2/CB2/CNR2 Antibody (3C7) - Azide and BSA Free

Western Blot: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Analysis of CNR2 expression in transfected 293T cell line by CNR2 monoclonal antibody (M01), clone 3C7.Lane 1: CNR2 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Analysis of monoclonal antibody to CNR2 on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Detection limit for recombinant GST tagged CNR2 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cannabinoid R2/CB2/CNR2 Antibody (3C7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CNR2 (NP_001832.1, 302 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RSGEIRSSAHHCLAHWKKCVRGLGSEAKEEAPRSSVTETEADGKITPWPDSRDLDLSDC
• Specificity: CNR2 (3C7)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNR2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cannabinoid R2/CB2/CNR2 Antibody (3C7) - Azide and BSA Free

• Cannabinoid R2
• cannabinoid receptor 2 (macrophage)
• CannabinoidR2
• CB-2
• CB2cannabinoid receptor 2
• CB2R
• CNR2
• CX5
• CX5testis-dominant CNR2 isoform CB2
• hCB2

Background

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cannabinoid R2/CB2/CNR2 Antibody (H00001269-M01)(4)

Publications using H00001269-M01

• Monika V, Emil B, Petr P et al. Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival. Transl Lung Cancer Res. 2022-08-11 [PMID: 36386452]
• Gandhi K, Montoya-Uribe V, Martinez S1 et al. Ontogeny and programming of the fetal temporal cortical endocannabinoid system by moderate maternal nutrient reduction in baboons (Papio spp.). Physiol Rep. 2019-01-01 [PMID: 30912236]
• Gandhi K, Li C, German N et al. Effect of maternal high-fat diet on key components of the placental and hepatic endocannabinoid system American Journal of Physiology-Endocrinology and Metabolism 2018-04-01 [PMID: 29138223]
• Brocato B, Zoerner AA, Janjetovic Z et al. Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity. Placenta. 2013-09-02 [PMID: 24008071]

Bioinformatics

• Gene Symbol: CNR2
• Entrez:
  • 1269(Human)
• OMIM:
  • 605051(Human)
• Uniprot:
  • NP_001832.1(Human)