Monoglyceride Lipase Antibody (OTI2B11)

Images

 
Western Blot: Monoglyceride Lipase Antibody (OTI2B11) [NBP2-00735] - Analysis of extracts (10ug) from HeLa cell line, dilution(1:200).
Immunocytochemistry/ Immunofluorescence: Monoglyceride Lipase Antibody (OTI2B11) [NBP2-00735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human prostate tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Flow Cytometry: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Monoglyceride ...read more
Western Blot: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Monoglyceride Lipase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human Kidney tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human pancreas tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

Monoglyceride Lipase Antibody (OTI2B11) Summary

Immunogen
Full length human recombinant protein of human MGLL (NP_009214) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MGLL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Monoglyceride Lipase Antibody (OTI2B11)

  • HUK5
  • HU-K5EC 3.1.1.23
  • Lysophospholipase homolog
  • Lysophospholipase-like
  • MAGL
  • MGLHUK5
  • MGLL
  • Monoacylglycerol lipase
  • Monoglyceride Lipase

Background

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MGLL
Entrez