Recombinant Human Protocadherin alpha 4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Protocadherin alpha 4 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells.

The ED50 for this effect is 0.025‑0.1 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived human Protocadherin alpha 4 protein
Met1-Asn697, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln30 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
PCDHA4
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
73.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80‑100 kDa, reducing conditions
Publications
Read Publication using
6509-CA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Protocadherin alpha 4 Protein, CF

  • CNR1
  • CNRN1
  • CRNR1
  • KIAA0345-like 10
  • MGC138307
  • MGC142169
  • ortholog of mouse CNR1
  • PCDHA4
  • PCDH-ALPHA4
  • PCDH-alpha-4
  • Protocadherin alpha 4
  • protocadherin alpha-4

Background

Protocadherin alpha 4 (PCDHA4), also called CNR1 (cadherin‑related neuronal receptor 1) in mouse, is a 110 ‑ 120 kDa type I transmembrane glycoprotein in the protocadherin family of more than 70 calcium‑dependent adhesion/recognition molecules (1 ‑ 4). It is one of 14 isoforms encoded by a tandemly arranged gene cluster of protocadherin alpha genes, and is thus termed a clustered protocadherin. Isoforms show monoallelic and combinatorial expression in the synaptic membrane of differentiating neurons that is thought to contribute to neural pathway recognition and sorting (5 ‑ 7). All isoforms have a similar, but non‑identical extracellular region with 6 cadherin repeats, and a constant region at the C‑terminus within the cytoplasmic domain indicating that all mediates similar intracellular signaling (4). Splice variants lacking the cytoplasmic constant region are common in clustered protocadherins; for PCDHA4 such a variant (798 aa versus the full‑length 947 aa) has been described (4, 8). Protocadherins are widely expressed in neurons, localized to axons and synaptic junctions (5, 9). Crystal structures indicate that PCDHA4 and other clustered cadherins are not likely to participate in the homophilic adhesions that are typical of cadherins, but participate in heterophilic interactions with gamma ‑protocadherins in cis that are needed for surface expression of alpha ‑protocadherin molecules (10, 11). Within the extracellular domain, human PCDHA4 shares 84% and 83% aa sequence identity with mouse and rat PCDHA4, respectively. An RGD motif in the first cadherin repeat of mouse PCDHA is thought to mediate beta 1 integrin adhesion, but is not present in human PCDHA4 (8, 10, 12). alpha ‑ and gamma ‑protocadherin molecules can be cleaved by matrix metalloproteinases and presenilin to generate a soluble extracellular domain and an intracellular portion that may be involved in signaling (1, 2).

  1. Morishita, H. and T. Yagi (2007) Curr. Opin. Cell Biol. 19:584.
  2. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
  3. Yagi, T. (2008) Develop. Growth Differ. 50:S131.
  4. Wu, Q. and T. Maniatis (1999) Cell 97:779.
  5. Noguchi, Y. et al. (2009) J. Biol. Chem. 284:32002.
  6. Kaneko, R. et al. (2006) J. Biol. Chem. 281:30551.
  7. Hasegawa, S. et al. (2008) Mol. Cell. Neurosci. 38:66.
  8. SwissProt Accession # Q9UN74.
  9. Katori, S. et al. (2009) J. Neurosci. 29:9137.
  10. Morishita, H. et al. (2006) J. Biol. Chem. 281:33650.
  11. Murata, Y et al. (2004) J. Biol. Chem. 279:49508.
  12. Mutoh, T. et al. (2004) Exp. Cell Res. 294:494.

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    Publications for Protocadherin alpha 4 (6509-CA)(1)

    We have publications tested in 1 confirmed species: Human.

    We have publications tested in 1 application: Bioassay.


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    Bioinformatics

    Gene Symbol PCDHA4
    Entrez
    Uniprot