F4/80 Antibody (BM8) [PE]

Images

 
Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [PE] [NBP2-22134] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS ...read more
Flow Cytometry: F4/80 Antibody (BM8) [PE] [NBP2-22134] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with F4/80 Antibody (BM8) and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
BM8
Clonality
Monoclonal
Host
Rat
Conjugate
PE

Order Details

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Catalog# & Conjugate Size Price

F4/80 Antibody (BM8) [PE] Summary

Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Marker
Macrophage Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody works in Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-22134 in the following applications:

Reactivity Notes

The monoclonal antibody BM8 cross reacts with human heart macrophages.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 1.0% BSA
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for F4/80 Antibody (BM8) [PE]

  • cell surface glycoprotein F4/80
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • EMR1 hormone receptor
  • F4/80
  • Gpf480
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7LN3

Background

The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (NBP2-22134) (0)

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Reviews for F4/80 Antibody (NBP2-22134) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP2-22134:
Filter by Applications
IHC-Fr
(1)
Flow
(1)
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Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen F4/80 NBP2-22134
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reviewed by:
Jim Hsiao
IHC-Fr Mouse 03/02/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLung tissue
SpeciesMouse
Lot14910M1113-C

Comments

CommentsFrozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature.
Flow Cytometry F4/80 NBP2-22134
Enlarge
reviewed by:
Jim Hsiao
Flow Mouse 03/02/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse splenocytes
SpeciesMouse
Lot14910M1113-C

Comments

CommentsSplenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with F4/80 Antibody (BM8) and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for F4/80 Antibody (NBP2-22134). (Showing 1 - 3 of 3 FAQ).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Jim Hsiao
03/02/2018
Application: IHC-Fr
Species: Mouse

Jim Hsiao
03/02/2018
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol EMR1
Entrez