F4/80 Antibody (BM8) [PE] Summary
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C in the dark.
PE (A=488, E=575)
PBS and 1.0% BSA
0.02% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A or G purified
- Western Blot 1:100-1:2000
- Flow Cytometry 1:10-1:1000
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
This antibody works in Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Alternate Names for F4/80 Antibody (BM8) [PE]
- cell surface glycoprotein F4/80
- EGF-like module containing, mucin-like, hormone receptor-like sequence 1
- EGF-like module receptor 1
- EGF-like module-containing mucin-like hormone receptor-like 1
- EMR1 hormone receptor
- lymphocyte antigen 71
- Mouse EMR1
- Rat EMR1
The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
Applications: Flow, IHC, IHC-P, IP
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
Publications for F4/80 Antibody (NBP2-22134) (0)
There are no publications for F4/80 Antibody (NBP2-22134).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for F4/80 Antibody (NBP2-22134) (0)
There are no reviews for F4/80 Antibody (NBP2-22134).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for F4/80 Antibody (NBP2-22134). (Showing 1 - 3 of 3 FAQ).
Are the F4/80/EMR1 antibodies validated in Simple Western?
- No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
Does F4/80/EMR1 antibodies come in lyophilized form?
- Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
What is the theoretical molecular weight for F4/80/EMR1 antibodies?
- The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
Additional F4/80 Antibody (BM8) [PE] Products
Related Products by Gene
Bioinformatics Tool for F4/80 Antibody (NBP2-22134)
Discover related pathways, diseases and genes to F4/80 Antibody (NBP2-22134). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on F4/80