F-Spondin/SPON1 Antibody - BSA Free

Western Blot: F-Spondin/SPON1 Antibody [NB300-339] - E coli-derived fusion protein as test antigen. Affi-anti- SPON1 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SPON1 )

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF (-)
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.91 mg/ml

F-Spondin/SPON1 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken F-Spondin/SPON1 Antibody - BSA Free (NB300-339) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide, corresponding to amino acids 549-807 of SPON1.
• Localization: Secreted, Extracellular Matrix
• Specificity: SPON1
• Clonality: Polyclonal
• Host: Chicken
• Gene: SPON1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200 - 1:500
• Application Notes: In Western Blot predicted molecular weight 100.9 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.91 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for F-Spondin/SPON1 Antibody - BSA Free

• FSpondin
• F-Spondin
• KIAA0762VSGP/F-spondin
• MGC10724
• SPON1
• spondin 1, (f-spondin) extracellular matrix protein
• spondin 1, extracellular matrix protein
• spondin-1
• Vascular smooth muscle cell growth-promoting factor
• VSGP

Background

SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPON1
• Entrez:
  • 104062(Mouse)
  • 64456(Rat)