Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody - Azide and BSA Free

Western Blot: Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody [NBP3-16959] - Western blot analysis of extracts of various cell lines, using Ezrin/Radixin/Moesin antibody (NBP3-16959) at 1:1000 dilution.HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody - Azide and BSA Free (NBP3-16959) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around T567 of human Ezrin/Radixin/Moesin (NP_003370.2). YKTLR
• Modification: p Thr567, p Thr564, p Thr558
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EZR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Application Notes: Modifications: Ezrin - p Thr 567, Radixin - p Thr 564, Moesin - p Thr 558

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Ezrin/Radixin/Moesin [p Thr567, p Thr564, p Thr558] Antibody - Azide and BSA Free

• CVIL
• CVL
• Cytovillin
• DFNB24
• EZR
• HEL70
• HEL-S-105
• IMD50
• Membrane-organizing extension spike protein
• MSN
• p81
• RDX
• villin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EZR