Ezrin [p Thr567] Antibody

Images

 
Western Blot: Ezrin [p Thr567] Antibody [NB100-81918] - Analysis of extracts from MDA cells untreated or treated with anisomycin using Ezrin(Phospho-Thr567) antibody
Western Blot: Ezrin [p Thr567] Antibody [NB100-81918] - Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody ( Lane 1 and 2) and Ezrin ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Ezrin [p Thr567] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 567 (Y-K-TP-L-R).
Modification
p Thr567
Specificity
Ezrin (specific to Phospho-Tyr567) detects endogenous levels of Ezrin only when phosphorylated at threonine 566.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Ezrin Lysate (NBL1-10401)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ezrin [p Thr567] Antibody

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EZR
Entrez
Uniprot