Ezrin Antibody (CL2384) - BSA Free

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Analysis of extracts from U-251 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-EZR monoclonal antibody.Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U251 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in HeLa cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human cell line A431 showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in MCF7 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U2OS cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human placenta shows strong positivity in apical membrane in trophoblastic cells.

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human tonsil shows strong membranous positivity in germinal center cells.

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL2384
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Ezrin Antibody (CL2384) - BSA Free Summary

• Description: Novus Biologicals Mouse Ezrin Antibody (CL2384) - BSA Free (NBP2-36760) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P15311, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EZR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Ezrin Antibody (CL2384) - BSA Free

• CVIL
• CVL
• Cytovillin 2
• Cytovillin
• DKFZp762H157
• EZR
• Ezrin
• FLJ26216
• p81
• VIL2
• VIL2MGC1584
• villin 2 (ezrin)
• Villin 2
• villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EZR
• Uniprot:
  • P15311(Human)